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Proteomic analysis of baicalin intervention on protein expression and modification in the hippocampus of Alzheimer's disease model rat.

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Title: Proteomic analysis of baicalin intervention on protein expression and modification in the hippocampus of Alzheimer's disease model rat.
Authors: Zhao, Jiwei (AUTHOR), Lu, Fan (AUTHOR), Yu, Hongli (AUTHOR), Cao, Jingwei (AUTHOR), Su, Zhiqiang (AUTHOR), Zhao, Jingkun (AUTHOR)
Source: International Journal of Neuroscience. Jul2025, Vol. 135 Issue 7, p797-806. 10p.
Subjects: Fatty acid desaturase, Protein expression, Laboratory rats, Maze tests, Alzheimer's disease
Abstract: Objective: We aimed to explore the treatment effect and therapeutic mechanisms of baicalin in Alzheimer's disease (AD). Methods: The AD rat model was established by intracerebroventricular injection of Aβ1-40, with rats in the baicalin group receiving baicalin intraventricular injections. Morris Water Maze and Hematoxylin-eosin (H&E) Staining were employed to detect the successful model construction and baicalin treatment effect. The proteins extracted from the hippocampus were subjected to proteomics analysis. Bioinformatics technology was employed for differential protein screening, functional classification, and enrichment. Western Blot was employed to validate the expressions of differentially expressed proteins (DEPs) and the protein modification alternations. Results: Water maze test confirmed the successful AD model construction and baicalin can improve learning and memory abilities. A total of 26 DEPs associated with 28 Gene Ontology (GO) functions were identified in the model and 32 DEPs were obtained between the baicalin group and the model. Bioinformatics analysis demonstrated that AD occurrence resulted in neuronal dysfunction and was associated with immune responses. The baicalin therapeutic effect on AD may be associated with metabolic processes, vitamin response, angiogenesis regulation, and fatty acid response. Immunoglobulin heavy constant mu (Ighm) and Immunoglobulin G2a (IgG2a) exhibited significant increases in AD and baicalin attenuated their expressions, while Fatty acid desaturase 1 (Fads1) exhibited a significantly diminished expression and baicalin could reverse the trend. Succinylation detection exhibited the differentially expressed at 35 kD between the model and baicalin group. Conclusion: Baicalin intervention may ameliorate cognitive impairment in AD rats by modulating the expressions of proteins and the succinylation modifications. [ABSTRACT FROM AUTHOR]
Copyright of International Journal of Neuroscience is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Label: Title
  Group: Ti
  Data: Proteomic analysis of baicalin intervention on protein expression and modification in the hippocampus of Alzheimer's disease model rat.
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  Data: <searchLink fieldCode="AR" term="%22Zhao%2C+Jiwei%22">Zhao, Jiwei</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lu%2C+Fan%22">Lu, Fan</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yu%2C+Hongli%22">Yu, Hongli</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cao%2C+Jingwei%22">Cao, Jingwei</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Su%2C+Zhiqiang%22">Su, Zhiqiang</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhao%2C+Jingkun%22">Zhao, Jingkun</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22International+Journal+of+Neuroscience%22">International Journal of Neuroscience</searchLink>. Jul2025, Vol. 135 Issue 7, p797-806. 10p.
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  Data: <searchLink fieldCode="DE" term="%22Fatty+acid+desaturase%22">Fatty acid desaturase</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+expression%22">Protein expression</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+rats%22">Laboratory rats</searchLink><br /><searchLink fieldCode="DE" term="%22Maze+tests%22">Maze tests</searchLink><br /><searchLink fieldCode="DE" term="%22Alzheimer's+disease%22">Alzheimer's disease</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Objective: We aimed to explore the treatment effect and therapeutic mechanisms of baicalin in Alzheimer's disease (AD). Methods: The AD rat model was established by intracerebroventricular injection of Aβ1-40, with rats in the baicalin group receiving baicalin intraventricular injections. Morris Water Maze and Hematoxylin-eosin (H&E) Staining were employed to detect the successful model construction and baicalin treatment effect. The proteins extracted from the hippocampus were subjected to proteomics analysis. Bioinformatics technology was employed for differential protein screening, functional classification, and enrichment. Western Blot was employed to validate the expressions of differentially expressed proteins (DEPs) and the protein modification alternations. Results: Water maze test confirmed the successful AD model construction and baicalin can improve learning and memory abilities. A total of 26 DEPs associated with 28 Gene Ontology (GO) functions were identified in the model and 32 DEPs were obtained between the baicalin group and the model. Bioinformatics analysis demonstrated that AD occurrence resulted in neuronal dysfunction and was associated with immune responses. The baicalin therapeutic effect on AD may be associated with metabolic processes, vitamin response, angiogenesis regulation, and fatty acid response. Immunoglobulin heavy constant mu (Ighm) and Immunoglobulin G2a (IgG2a) exhibited significant increases in AD and baicalin attenuated their expressions, while Fatty acid desaturase 1 (Fads1) exhibited a significantly diminished expression and baicalin could reverse the trend. Succinylation detection exhibited the differentially expressed at 35 kD between the model and baicalin group. Conclusion: Baicalin intervention may ameliorate cognitive impairment in AD rats by modulating the expressions of proteins and the succinylation modifications. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of International Journal of Neuroscience is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1080/00207454.2024.2332963
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      – Code: eng
        Text: English
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        PageCount: 10
        StartPage: 797
    Subjects:
      – SubjectFull: Fatty acid desaturase
        Type: general
      – SubjectFull: Protein expression
        Type: general
      – SubjectFull: Laboratory rats
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      – SubjectFull: Maze tests
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      – SubjectFull: Alzheimer's disease
        Type: general
    Titles:
      – TitleFull: Proteomic analysis of baicalin intervention on protein expression and modification in the hippocampus of Alzheimer's disease model rat.
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            NameFull: Zhao, Jiwei
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            NameFull: Lu, Fan
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            NameFull: Yu, Hongli
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            NameFull: Cao, Jingwei
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            NameFull: Su, Zhiqiang
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            NameFull: Zhao, Jingkun
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            – D: 01
              M: 07
              Text: Jul2025
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              Y: 2025
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