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Electrical Remodeling of Pressure Overloaded Rat Heart Is Attenuated if Imposed During Proliferative Cardiac Growth.

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Title: Electrical Remodeling of Pressure Overloaded Rat Heart Is Attenuated if Imposed During Proliferative Cardiac Growth.
Authors: Nekvindova, Eva (AUTHOR), Hrdlicka, Jaroslav (AUTHOR), Boros, Almos (AUTHOR), Slegrova, Michaela (AUTHOR), Kvasilova, Alena (AUTHOR), Skop, Vojtech (AUTHOR), Halberstat, Jan (AUTHOR), Holzerova, Kristyna (AUTHOR), Neckar, Jan (AUTHOR), Sedmera, David (AUTHOR), Olejnickova, Veronika (AUTHOR)
Source: Acta Physiologica. Nov2025, Vol. 241 Issue 11, p1-18. 18p.
Subjects: Connexin 43, Electrophysiology, Action potentials, Lipidomics, High pressure (Science), Echocardiography, Cardiovascular development
Abstract: Aim: Left ventricular pressure overload (LVPO) in adults is associated with adverse electrical remodeling, characterized by reduced conduction velocity (CV). However, the progression of LVPO differs when imposed during the proliferative phase of cardiac development. It remains unknown how increased cardiomyocyte proliferation affects LVPO electrical remodeling. Methods: CV maturation from rat postnatal day (PD) 1 to PD90 and analyzed underlying connexin 43 (Cx43) profile. Pressure overload was induced by abdominal aortic constriction (AAC) in rats during the proliferative phase of cardiac growth (PD2). Animals subjected to AAC during the non‐proliferative heart growth (AAC‐PD6) and Sham‐operated rats served as controls. Electrical remodeling was assessed at PD21 using ECG, optical mapping, western blots, immunofluorescence, and lipidomic analysis, complemented by functional analyses through echocardiography. Results: Pressure overload led to a 2.5‐fold increase in heart weight compared to Sham in both AAC groups. A significant increase in relative left ventricular wall thickening was observed in AAC‐PD2 rats only. Optical mapping and ECG showed preserved conduction properties in AAC‐PD2 animals, whereas the AAC‐PD6 group displayed prolonged QRS and significantly reduced longitudinal CV. While total and phosphorylated Cx43 levels were comparable between the AAC groups, AAC‐PD2 animals demonstrated higher intercalated disc localization. Furthermore, lipidomic profiling revealed maintained long‐chain acylcarnitine (LCAC) levels in AAC‐PD2, whereas AAC‐PD6 tended toward LCAC accumulation. Conclusion: This study provides new insights into the remodeling upon pressure overload during cardiac proliferative growth, demonstrating attenuated electrical alteration by preserved CV and highlighting the role of Cx43 localization and preserved levels of LCACs. [ABSTRACT FROM AUTHOR]
Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Label: Title
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  Data: Electrical Remodeling of Pressure Overloaded Rat Heart Is Attenuated if Imposed During Proliferative Cardiac Growth.
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  Data: <searchLink fieldCode="AR" term="%22Nekvindova%2C+Eva%22">Nekvindova, Eva</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hrdlicka%2C+Jaroslav%22">Hrdlicka, Jaroslav</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Boros%2C+Almos%22">Boros, Almos</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Slegrova%2C+Michaela%22">Slegrova, Michaela</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kvasilova%2C+Alena%22">Kvasilova, Alena</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Skop%2C+Vojtech%22">Skop, Vojtech</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Halberstat%2C+Jan%22">Halberstat, Jan</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Holzerova%2C+Kristyna%22">Holzerova, Kristyna</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Neckar%2C+Jan%22">Neckar, Jan</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sedmera%2C+David%22">Sedmera, David</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Olejnickova%2C+Veronika%22">Olejnickova, Veronika</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Acta+Physiologica%22">Acta Physiologica</searchLink>. Nov2025, Vol. 241 Issue 11, p1-18. 18p.
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  Data: <searchLink fieldCode="DE" term="%22Connexin+43%22">Connexin 43</searchLink><br /><searchLink fieldCode="DE" term="%22Electrophysiology%22">Electrophysiology</searchLink><br /><searchLink fieldCode="DE" term="%22Action+potentials%22">Action potentials</searchLink><br /><searchLink fieldCode="DE" term="%22Lipidomics%22">Lipidomics</searchLink><br /><searchLink fieldCode="DE" term="%22High+pressure+%28Science%29%22">High pressure (Science)</searchLink><br /><searchLink fieldCode="DE" term="%22Echocardiography%22">Echocardiography</searchLink><br /><searchLink fieldCode="DE" term="%22Cardiovascular+development%22">Cardiovascular development</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Aim: Left ventricular pressure overload (LVPO) in adults is associated with adverse electrical remodeling, characterized by reduced conduction velocity (CV). However, the progression of LVPO differs when imposed during the proliferative phase of cardiac development. It remains unknown how increased cardiomyocyte proliferation affects LVPO electrical remodeling. Methods: CV maturation from rat postnatal day (PD) 1 to PD90 and analyzed underlying connexin 43 (Cx43) profile. Pressure overload was induced by abdominal aortic constriction (AAC) in rats during the proliferative phase of cardiac growth (PD2). Animals subjected to AAC during the non‐proliferative heart growth (AAC‐PD6) and Sham‐operated rats served as controls. Electrical remodeling was assessed at PD21 using ECG, optical mapping, western blots, immunofluorescence, and lipidomic analysis, complemented by functional analyses through echocardiography. Results: Pressure overload led to a 2.5‐fold increase in heart weight compared to Sham in both AAC groups. A significant increase in relative left ventricular wall thickening was observed in AAC‐PD2 rats only. Optical mapping and ECG showed preserved conduction properties in AAC‐PD2 animals, whereas the AAC‐PD6 group displayed prolonged QRS and significantly reduced longitudinal CV. While total and phosphorylated Cx43 levels were comparable between the AAC groups, AAC‐PD2 animals demonstrated higher intercalated disc localization. Furthermore, lipidomic profiling revealed maintained long‐chain acylcarnitine (LCAC) levels in AAC‐PD2, whereas AAC‐PD6 tended toward LCAC accumulation. Conclusion: This study provides new insights into the remodeling upon pressure overload during cardiac proliferative growth, demonstrating attenuated electrical alteration by preserved CV and highlighting the role of Cx43 localization and preserved levels of LCACs. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/apha.70118
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      – Code: eng
        Text: English
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      – SubjectFull: Connexin 43
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      – SubjectFull: Electrophysiology
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      – SubjectFull: Action potentials
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      – SubjectFull: Echocardiography
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      – SubjectFull: Cardiovascular development
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              Text: Nov2025
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