View in EDS HTML Full Text PDF Full Text

Lariat RNA debranching prevents harmful siRNA burst in plants.

Saved in:
Bibliographic Details
Title: Lariat RNA debranching prevents harmful siRNA burst in plants.
Authors: Tang, Qi (AUTHOR), Ding, Chenxi (AUTHOR), Zhang, Xiaotuo (AUTHOR), Wang, Taiyun (AUTHOR), Zhu, Mengjie (AUTHOR), Cui, Ruixue (AUTHOR), Yang, Wenya (AUTHOR), Ma, Jinbiao (AUTHOR), Ren, Guodong (AUTHOR), Zhang, Xiaoming (AUTHOR), Zheng, Binglian (AUTHOR)
Source: Science. 6/25/2026, Vol. 392 Issue 6805, p1401-1407. 7p.
Subjects: Small interfering RNA, Disease resistance of plants, RNA splicing, RNA synthesis, Non-coding RNA, Enzymes, Arabidopsis
Abstract: Lariat RNAs are formed from introns during pre–messenger RNA splicing, after which they are degraded by the debranching enzyme DBR1. Impairment of DBR1 leads to developmental arrest, yet the mechanism remains unclear. In this study, we found that debranching of lariat RNA prevents production of 21- and 22-nucleotide lariat-derived small interfering RNAs (lasiRNAs), which causes a burst of exonic siRNAs, thereby safeguarding development and defense response. LasiRNA biogenesis relied on RNA-dependent RNA polymerases and Dicer-like proteins (DCLs). Upon pathogen infection and dysfunction of DBR1, many lariat RNAs were hijacked by DCL4 and DCL2 and processed into siRNAs that particularly target immunity genes, ultimately disrupting plant defense responses. Collectively, DBR1-mediated lariat RNA removal serves as a protective mechanism to prevent the activation of a small RNA–based defense system in plants. Editor's summary: Lariat RNAs are generated from excised introns during splicing of pre-messenger RNAs. They are rapidly degraded by the debranching enzyme DBR1. Using a partial loss-of-function dbr1 mutant in the model plant Arabidopsis, Tang et al. found that lariat RNAs can be processed into 21- and 22-nucleotide small interfering RNAs, which they named lasiRNAs. In the absence of DBR1, lariat RNAs are preferentially processed by the dicer-like protein DCL4 into 21-nucleotide lasiRNAs. However, when DCL4 function is compromised, such as during viral infection, lariat RNAs are processed by DCL2 into 22-nucleotide lasiRNAs, which in turn repress immunity genes through exonic small interfering RNA burst. —Unnati Sonawala and Madeleine Seale [ABSTRACT FROM AUTHOR]
Copyright of Science is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: pbh
DbLabel: Psychology and Behavioral Sciences Collection
An: 194869302
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Lariat RNA debranching prevents harmful siRNA burst in plants.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Tang%2C+Qi%22">Tang, Qi</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ding%2C+Chenxi%22">Ding, Chenxi</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Xiaotuo%22">Zhang, Xiaotuo</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Taiyun%22">Wang, Taiyun</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhu%2C+Mengjie%22">Zhu, Mengjie</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cui%2C+Ruixue%22">Cui, Ruixue</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yang%2C+Wenya%22">Yang, Wenya</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ma%2C+Jinbiao%22">Ma, Jinbiao</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ren%2C+Guodong%22">Ren, Guodong</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Xiaoming%22">Zhang, Xiaoming</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zheng%2C+Binglian%22">Zheng, Binglian</searchLink> (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Science%22">Science</searchLink>. 6/25/2026, Vol. 392 Issue 6805, p1401-1407. 7p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Small+interfering+RNA%22">Small interfering RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Disease+resistance+of+plants%22">Disease resistance of plants</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+splicing%22">RNA splicing</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+synthesis%22">RNA synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Non-coding+RNA%22">Non-coding RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Enzymes%22">Enzymes</searchLink><br /><searchLink fieldCode="DE" term="%22Arabidopsis%22">Arabidopsis</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Lariat RNAs are formed from introns during pre–messenger RNA splicing, after which they are degraded by the debranching enzyme DBR1. Impairment of DBR1 leads to developmental arrest, yet the mechanism remains unclear. In this study, we found that debranching of lariat RNA prevents production of 21- and 22-nucleotide lariat-derived small interfering RNAs (lasiRNAs), which causes a burst of exonic siRNAs, thereby safeguarding development and defense response. LasiRNA biogenesis relied on RNA-dependent RNA polymerases and Dicer-like proteins (DCLs). Upon pathogen infection and dysfunction of DBR1, many lariat RNAs were hijacked by DCL4 and DCL2 and processed into siRNAs that particularly target immunity genes, ultimately disrupting plant defense responses. Collectively, DBR1-mediated lariat RNA removal serves as a protective mechanism to prevent the activation of a small RNA–based defense system in plants. Editor's summary: Lariat RNAs are generated from excised introns during splicing of pre-messenger RNAs. They are rapidly degraded by the debranching enzyme DBR1. Using a partial loss-of-function dbr1 mutant in the model plant Arabidopsis, Tang et al. found that lariat RNAs can be processed into 21- and 22-nucleotide small interfering RNAs, which they named lasiRNAs. In the absence of DBR1, lariat RNAs are preferentially processed by the dicer-like protein DCL4 into 21-nucleotide lasiRNAs. However, when DCL4 function is compromised, such as during viral infection, lariat RNAs are processed by DCL2 into 22-nucleotide lasiRNAs, which in turn repress immunity genes through exonic small interfering RNA burst. —Unnati Sonawala and Madeleine Seale [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Science is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=194869302
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1126/science.aeb4938
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 7
        StartPage: 1401
    Subjects:
      – SubjectFull: Small interfering RNA
        Type: general
      – SubjectFull: Disease resistance of plants
        Type: general
      – SubjectFull: RNA splicing
        Type: general
      – SubjectFull: RNA synthesis
        Type: general
      – SubjectFull: Non-coding RNA
        Type: general
      – SubjectFull: Enzymes
        Type: general
      – SubjectFull: Arabidopsis
        Type: general
    Titles:
      – TitleFull: Lariat RNA debranching prevents harmful siRNA burst in plants.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Tang, Qi
      – PersonEntity:
          Name:
            NameFull: Ding, Chenxi
      – PersonEntity:
          Name:
            NameFull: Zhang, Xiaotuo
      – PersonEntity:
          Name:
            NameFull: Wang, Taiyun
      – PersonEntity:
          Name:
            NameFull: Zhu, Mengjie
      – PersonEntity:
          Name:
            NameFull: Cui, Ruixue
      – PersonEntity:
          Name:
            NameFull: Yang, Wenya
      – PersonEntity:
          Name:
            NameFull: Ma, Jinbiao
      – PersonEntity:
          Name:
            NameFull: Ren, Guodong
      – PersonEntity:
          Name:
            NameFull: Zhang, Xiaoming
      – PersonEntity:
          Name:
            NameFull: Zheng, Binglian
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 25
              M: 06
              Text: 6/25/2026
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-print
              Value: 00368075
          Numbering:
            – Type: volume
              Value: 392
            – Type: issue
              Value: 6805
          Titles:
            – TitleFull: Science
              Type: main
ResultId 1