GTP-dependent twisting of dynamin implicates constriction and tension in membrane fission.
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| Title: | GTP-dependent twisting of dynamin implicates constriction and tension in membrane fission. |
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| Authors: | Roux, Aurélien, Uyhazi, Katherine, Frost, Adam, De Camilli, Pietro |
| Source: | Nature. 5/25/2006, Vol. 441 Issue 7092, p528-531. 4p. 4 Color Photographs. |
| Subjects: | Guanosine triphosphate, Nucleotides, Hydrolysis, Endocytosis, Fission (Asexual reproduction) |
| Abstract: | Dynamin, a crucial factor in endocytosis, is a member of a family of GTPases that participates in membrane fission. It was initially proposed to act as a machine that constricts and cuts the neck of nascent vesicles in a GTP-hydrolysis-dependent reaction, but subsequent studies suggested alternative models. Here we monitored the effect of nucleotides on dynamin-coated lipid tubules in real time. Addition of GTP, but not of GDP or GTP-γS, resulted in twisting of the tubules and supercoiling, suggesting a rotatory movement of the helix turns relative to each other during GTP hydrolysis. Rotation was confirmed by the movement of beads attached to the tubules. Twisting activity produced a longitudinal tension that was released by tubule breakage when both ends of the tubule were anchored. Fission also occurred when dynamin and GTP were added to lipid tubules that had been generated from liposomes by the motor activity of kinesin on microtubules. No fission events were observed in the absence of longitudinal tension. These findings demonstrate a mechanoenzyme activity of dynamin in endocytosis, but also imply that constriction is not sufficient for fission. At the short necks of endocytic vesicles, other factors leading to tension may cooperate with the constricting activity of dynamin to induce fission. [ABSTRACT FROM AUTHOR] |
| Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 20927464 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: GTP-dependent twisting of dynamin implicates constriction and tension in membrane fission. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Roux%2C+Aurélien%22">Roux, Aurélien</searchLink><br /><searchLink fieldCode="AR" term="%22Uyhazi%2C+Katherine%22">Uyhazi, Katherine</searchLink><br /><searchLink fieldCode="AR" term="%22Frost%2C+Adam%22">Frost, Adam</searchLink><br /><searchLink fieldCode="AR" term="%22De+Camilli%2C+Pietro%22">De Camilli, Pietro</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Nature%22">Nature</searchLink>. 5/25/2006, Vol. 441 Issue 7092, p528-531. 4p. 4 Color Photographs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Guanosine+triphosphate%22">Guanosine triphosphate</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleotides%22">Nucleotides</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrolysis%22">Hydrolysis</searchLink><br /><searchLink fieldCode="DE" term="%22Endocytosis%22">Endocytosis</searchLink><br /><searchLink fieldCode="DE" term="%22Fission+%28Asexual+reproduction%29%22">Fission (Asexual reproduction)</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Dynamin, a crucial factor in endocytosis, is a member of a family of GTPases that participates in membrane fission. It was initially proposed to act as a machine that constricts and cuts the neck of nascent vesicles in a GTP-hydrolysis-dependent reaction, but subsequent studies suggested alternative models. Here we monitored the effect of nucleotides on dynamin-coated lipid tubules in real time. Addition of GTP, but not of GDP or GTP-γS, resulted in twisting of the tubules and supercoiling, suggesting a rotatory movement of the helix turns relative to each other during GTP hydrolysis. Rotation was confirmed by the movement of beads attached to the tubules. Twisting activity produced a longitudinal tension that was released by tubule breakage when both ends of the tubule were anchored. Fission also occurred when dynamin and GTP were added to lipid tubules that had been generated from liposomes by the motor activity of kinesin on microtubules. No fission events were observed in the absence of longitudinal tension. These findings demonstrate a mechanoenzyme activity of dynamin in endocytosis, but also imply that constriction is not sufficient for fission. At the short necks of endocytic vesicles, other factors leading to tension may cooperate with the constricting activity of dynamin to induce fission. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1038/nature04718 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 4 StartPage: 528 Subjects: – SubjectFull: Guanosine triphosphate Type: general – SubjectFull: Nucleotides Type: general – SubjectFull: Hydrolysis Type: general – SubjectFull: Endocytosis Type: general – SubjectFull: Fission (Asexual reproduction) Type: general Titles: – TitleFull: GTP-dependent twisting of dynamin implicates constriction and tension in membrane fission. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Roux, Aurélien – PersonEntity: Name: NameFull: Uyhazi, Katherine – PersonEntity: Name: NameFull: Frost, Adam – PersonEntity: Name: NameFull: De Camilli, Pietro IsPartOfRelationships: – BibEntity: Dates: – D: 25 M: 05 Text: 5/25/2006 Type: published Y: 2006 Identifiers: – Type: issn-print Value: 00280836 Numbering: – Type: volume Value: 441 – Type: issue Value: 7092 Titles: – TitleFull: Nature Type: main |
| ResultId | 1 |
