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Competition between glucose and lactate as oxidative energy substrates in both neurons and astrocytes: a comparative NMR study.

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Title: Competition between glucose and lactate as oxidative energy substrates in both neurons and astrocytes: a comparative NMR study.
Authors: Bouzier‐Sore, Anne‐Karine (AUTHOR), Voisin, Pierre (AUTHOR), Bouchaud, Véronique (AUTHOR), Bezancon, Eric (AUTHOR), Franconi, Jean‐Michel (AUTHOR), Pellerin, Luc (AUTHOR)
Source: European Journal of Neuroscience. Sep2006, Vol. 24 Issue 6, p1687-1694. 8p. 1 Diagram, 3 Charts, 2 Graphs.
Subjects: Astrocytes, Neurons, Glucose, Cell culture, Metabolism
Abstract: Competition between glucose and lactate as oxidative energy substrates was investigated in both primary cultures of astrocytes and neurons using physiological concentrations (1.1 mm for each). Glucose metabolism was distinguished from lactate metabolism by using alternatively labelled substrates in the medium ([1-13C]glucose + lactate or glucose + [3-13C]lactate). After 4 h of incubation, 1H and 13C-NMR spectra were realized on perchloric acid extracts of both cells and culture media. For astrocytic cultures, spectra showed that amino acids (glutamine and alanine) were more labelled in the glucose-labelled condition, indicating that glucose is a better substrate to support oxidative metabolism in these cells. The opposite was observed on spectra from neuronal cultures, glutamate being much more labelled in the lactate-labelled condition, confirming that neurons consume lactate preferentially as an oxidative energy substrate. Analysis of glutamine and glutamate peaks (singlets or multiplets) also suggests that astrocytes have a less active oxidative metabolism than neurons. In contrast, they exhibit a stronger glycolytic metabolism than neurons as indicated by their high lactate production yield. Using a mathematical model, we have estimated the relative contribution of exogenous glucose and lactate to neuronal oxidative metabolism. Under the aforementioned conditions, it represents 25% for glucose and 75% for lactate. Altogether, these results obtained on separate astrocytic and neuronal cultures support the idea that lactate, predominantly produced by astrocytes, is used as a supplementary fuel by neurons in vivo already under resting physiological conditions. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Competition between glucose and lactate as oxidative energy substrates in both neurons and astrocytes: a comparative NMR study.
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  Data: <searchLink fieldCode="AR" term="%22Bouzier‐Sore%2C+Anne‐Karine%22">Bouzier‐Sore, Anne‐Karine</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Voisin%2C+Pierre%22">Voisin, Pierre</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bouchaud%2C+Véronique%22">Bouchaud, Véronique</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bezancon%2C+Eric%22">Bezancon, Eric</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Franconi%2C+Jean‐Michel%22">Franconi, Jean‐Michel</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pellerin%2C+Luc%22">Pellerin, Luc</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Sep2006, Vol. 24 Issue 6, p1687-1694. 8p. 1 Diagram, 3 Charts, 2 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Astrocytes%22">Astrocytes</searchLink><br /><searchLink fieldCode="DE" term="%22Neurons%22">Neurons</searchLink><br /><searchLink fieldCode="DE" term="%22Glucose%22">Glucose</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+culture%22">Cell culture</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolism%22">Metabolism</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Competition between glucose and lactate as oxidative energy substrates was investigated in both primary cultures of astrocytes and neurons using physiological concentrations (1.1 mm for each). Glucose metabolism was distinguished from lactate metabolism by using alternatively labelled substrates in the medium ([1-13C]glucose + lactate or glucose + [3-13C]lactate). After 4 h of incubation, 1H and 13C-NMR spectra were realized on perchloric acid extracts of both cells and culture media. For astrocytic cultures, spectra showed that amino acids (glutamine and alanine) were more labelled in the glucose-labelled condition, indicating that glucose is a better substrate to support oxidative metabolism in these cells. The opposite was observed on spectra from neuronal cultures, glutamate being much more labelled in the lactate-labelled condition, confirming that neurons consume lactate preferentially as an oxidative energy substrate. Analysis of glutamine and glutamate peaks (singlets or multiplets) also suggests that astrocytes have a less active oxidative metabolism than neurons. In contrast, they exhibit a stronger glycolytic metabolism than neurons as indicated by their high lactate production yield. Using a mathematical model, we have estimated the relative contribution of exogenous glucose and lactate to neuronal oxidative metabolism. Under the aforementioned conditions, it represents 25% for glucose and 75% for lactate. Altogether, these results obtained on separate astrocytic and neuronal cultures support the idea that lactate, predominantly produced by astrocytes, is used as a supplementary fuel by neurons in vivo already under resting physiological conditions. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/j.1460-9568.2006.05056.x
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        Text: English
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        Type: general
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              M: 09
              Text: Sep2006
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