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Reduction in infectivity of endogenous transmissible spongiform encephalopathies present in blood by adsorption to selective affinity resins.

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Title: Reduction in infectivity of endogenous transmissible spongiform encephalopathies present in blood by adsorption to selective affinity resins.
Authors: Gregori, Luisa, Gurgel, Patrick V, Lathrop, Julia T, Edwardson, Peter, Lambert, Brian C, Carbonell, Ruben G, Burton, Steven J, Hammond, David J, Rohwer, Robert G
Source: Lancet. 12/23/2006, Vol. 368 Issue 9554, p2226-2230. 5p. 1 Chart, 1 Graph.
Subjects: Chronic wasting disease, Blood transfusion, Prevention of bloodborne infections, Gums & resins, Etiology of diseases, Hamsters as laboratory animals
Abstract: Summary Background Transmissible spongiform encephalopathies (TSE) can be contracted through blood transfusion. Selective adsorption of the causative agent from donated blood might be one of the best ways of managing this risk. In our study, affinity resin L13, which reduces brain-derived infectivity spiked into human red blood cell concentrate by around 4 log10ID50, and its equivalent, L13A, produced on a manufacturing scale, were assessed for their ability to remove TSE infectivity endogenously present in blood. Methods 500 mL of scrapie-infected hamster whole blood was leucoreduced at full scale before passage through the affinity resins. Infectivity of whole blood, leucoreduced whole blood (challenge), and the recovered blood from each flow-through was measured by limiting dilution titration. Findings Leucoreduction removed 72% of input infectivity. 15 of 99 animals were infected by the challenge, whereas none of the 96 or 100 animals inoculated with the final flow-throughs from either resin developed the disease after 540 days. The limit of detection of the bioassay was 0·2 infectious doses per mL. The overall reduction of the challenge infectivity was more than 1·22 log10ID. The results showed removal of endogenous TSE infectivity from leucoreduced whole blood by affinity ligands. The same resins adsorb normal and abnormal prion protein from human infections with variant, sporadic, and familial Creutzfeldt-Jakob disease, in the presence of blood components. Interpretation TSE affinity ligands, when incorporated into appropriate devices, can be used to mitigate the risks from TSE-infected blood, blood products, and other materials exposed to TSE infectivity. [ABSTRACT FROM AUTHOR]
Copyright of Lancet is the property of Lancet and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Reduction in infectivity of endogenous transmissible spongiform encephalopathies present in blood by adsorption to selective affinity resins.
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  Data: <searchLink fieldCode="AR" term="%22Gregori%2C+Luisa%22">Gregori, Luisa</searchLink><br /><searchLink fieldCode="AR" term="%22Gurgel%2C+Patrick+V%22">Gurgel, Patrick V</searchLink><br /><searchLink fieldCode="AR" term="%22Lathrop%2C+Julia+T%22">Lathrop, Julia T</searchLink><br /><searchLink fieldCode="AR" term="%22Edwardson%2C+Peter%22">Edwardson, Peter</searchLink><br /><searchLink fieldCode="AR" term="%22Lambert%2C+Brian+C%22">Lambert, Brian C</searchLink><br /><searchLink fieldCode="AR" term="%22Carbonell%2C+Ruben+G%22">Carbonell, Ruben G</searchLink><br /><searchLink fieldCode="AR" term="%22Burton%2C+Steven+J%22">Burton, Steven J</searchLink><br /><searchLink fieldCode="AR" term="%22Hammond%2C+David+J%22">Hammond, David J</searchLink><br /><searchLink fieldCode="AR" term="%22Rohwer%2C+Robert+G%22">Rohwer, Robert G</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Lancet%22">Lancet</searchLink>. 12/23/2006, Vol. 368 Issue 9554, p2226-2230. 5p. 1 Chart, 1 Graph.
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  Data: <searchLink fieldCode="DE" term="%22Chronic+wasting+disease%22">Chronic wasting disease</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+transfusion%22">Blood transfusion</searchLink><br /><searchLink fieldCode="DE" term="%22Prevention+of+bloodborne+infections%22">Prevention of bloodborne infections</searchLink><br /><searchLink fieldCode="DE" term="%22Gums+%26+resins%22">Gums & resins</searchLink><br /><searchLink fieldCode="DE" term="%22Etiology+of+diseases%22">Etiology of diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Hamsters+as+laboratory+animals%22">Hamsters as laboratory animals</searchLink>
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  Data: Summary Background Transmissible spongiform encephalopathies (TSE) can be contracted through blood transfusion. Selective adsorption of the causative agent from donated blood might be one of the best ways of managing this risk. In our study, affinity resin L13, which reduces brain-derived infectivity spiked into human red blood cell concentrate by around 4 log10ID50, and its equivalent, L13A, produced on a manufacturing scale, were assessed for their ability to remove TSE infectivity endogenously present in blood. Methods 500 mL of scrapie-infected hamster whole blood was leucoreduced at full scale before passage through the affinity resins. Infectivity of whole blood, leucoreduced whole blood (challenge), and the recovered blood from each flow-through was measured by limiting dilution titration. Findings Leucoreduction removed 72% of input infectivity. 15 of 99 animals were infected by the challenge, whereas none of the 96 or 100 animals inoculated with the final flow-throughs from either resin developed the disease after 540 days. The limit of detection of the bioassay was 0·2 infectious doses per mL. The overall reduction of the challenge infectivity was more than 1·22 log10ID. The results showed removal of endogenous TSE infectivity from leucoreduced whole blood by affinity ligands. The same resins adsorb normal and abnormal prion protein from human infections with variant, sporadic, and familial Creutzfeldt-Jakob disease, in the presence of blood components. Interpretation TSE affinity ligands, when incorporated into appropriate devices, can be used to mitigate the risks from TSE-infected blood, blood products, and other materials exposed to TSE infectivity. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Lancet is the property of Lancet and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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              Text: 12/23/2006
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