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Physiological Proteomics of the Uncultured Endosymbiont of Riftia pachyptila.

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Title: Physiological Proteomics of the Uncultured Endosymbiont of Riftia pachyptila.
Authors: Markert, Stephanie, Arndt, Cordelia, Felbeck, Horst, Becher, Dörte, Sievert, Stefan M., Hügler, Michael, Albrecht, Dirk, Robidart, Julie, Bench, Shellie, Feldman, Robert A., Hecker, Michael, Schweder, Thomas
Source: Science (pre-March 2025). 1/12/2007, Vol. 315 Issue 5809, p247-250. 4p.
Subjects: Proteomics, Pachyptila, Proteins, Biochemistry, Metabolism, Krebs cycle, Carbon dioxide, Molecular biology, Oxidation
Abstract: The bacterial endosymbiont of the deep-sea tube worm Riftia pachyptila has never been successfully cultivated outside its host. In the absence of cultivation data, we have taken a proteomic approach based on the metagenome sequence to study the metabolism of this peculiar microorganism in detail. As one result, we found that three major sulfide oxidation proteins constitute ∼12% of the total cytosolic proteome, which highlights the essential role of these enzymes for the symbiont's energy metabolism. Unexpectedly, the symbiont uses the reductive tricarboxylic acid cycle in addition to the previously identified Calvin cycle for CO2 fixation. [ABSTRACT FROM AUTHOR]
Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Physiological Proteomics of the Uncultured Endosymbiont of Riftia pachyptila.
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  Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 1/12/2007, Vol. 315 Issue 5809, p247-250. 4p.
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  Data: <searchLink fieldCode="DE" term="%22Proteomics%22">Proteomics</searchLink><br /><searchLink fieldCode="DE" term="%22Pachyptila%22">Pachyptila</searchLink><br /><searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemistry%22">Biochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolism%22">Metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Krebs+cycle%22">Krebs cycle</searchLink><br /><searchLink fieldCode="DE" term="%22Carbon+dioxide%22">Carbon dioxide</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+biology%22">Molecular biology</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidation%22">Oxidation</searchLink>
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  Data: The bacterial endosymbiont of the deep-sea tube worm Riftia pachyptila has never been successfully cultivated outside its host. In the absence of cultivation data, we have taken a proteomic approach based on the metagenome sequence to study the metabolism of this peculiar microorganism in detail. As one result, we found that three major sulfide oxidation proteins constitute ∼12% of the total cytosolic proteome, which highlights the essential role of these enzymes for the symbiont's energy metabolism. Unexpectedly, the symbiont uses the reductive tricarboxylic acid cycle in addition to the previously identified Calvin cycle for CO2 fixation. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=23773547
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        Value: 10.1126/science.1132913
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 4
        StartPage: 247
    Subjects:
      – SubjectFull: Proteomics
        Type: general
      – SubjectFull: Pachyptila
        Type: general
      – SubjectFull: Proteins
        Type: general
      – SubjectFull: Biochemistry
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      – SubjectFull: Metabolism
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      – SubjectFull: Krebs cycle
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      – SubjectFull: Carbon dioxide
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      – SubjectFull: Molecular biology
        Type: general
      – SubjectFull: Oxidation
        Type: general
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      – TitleFull: Physiological Proteomics of the Uncultured Endosymbiont of Riftia pachyptila.
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              Text: 1/12/2007
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