Intronic microRNA precursors that bypass Drosha processing.
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| Title: | Intronic microRNA precursors that bypass Drosha processing. |
|---|---|
| Authors: | Ruby, J. Graham, Jan, Calvin H., Bartel, David P. |
| Source: | Nature. 7/5/2007, Vol. 448 Issue 7149, p83-86. 4p. 1 Color Photograph, 2 Graphs. |
| Subjects: | Ribonucleases, Messenger RNA, Nucleotides, Introns, Split genes, Drosophila melanogaster |
| Abstract: | MicroRNAs (miRNAs) are ∼22-nucleotide endogenous RNAs that often repress the expression of complementary messenger RNAs. In animals, miRNAs derive from characteristic hairpins in primary transcripts through two sequential RNase III-mediated cleavages; Drosha cleaves near the base of the stem to liberate a ∼60-nucleotide pre-miRNA hairpin, then Dicer cleaves near the loop to generate a miRNA:miRNA* duplex. From that duplex, the mature miRNA is incorporated into the silencing complex. Here we identify an alternative pathway for miRNA biogenesis, in which certain debranched introns mimic the structural features of pre-miRNAs to enter the miRNA-processing pathway without Drosha-mediated cleavage. We call these pre-miRNAs/introns ‘mirtrons’, and have identified 14 mirtrons in Drosophila melanogaster and another four in Caenorhabditis elegans (including the reclassification of mir-62). Some of these have been selectively maintained during evolution with patterns of sequence conservation suggesting important regulatory functions in the animal. The abundance of introns comparable in size to pre-miRNAs appears to have created a context favourable for the emergence of mirtrons in flies and nematodes. This suggests that other lineages with many similarly sized introns probably also have mirtrons, and that the mirtron pathway could have provided an early avenue for the emergence of miRNAs before the advent of Drosha. [ABSTRACT FROM AUTHOR] |
| Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 25621569 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Intronic microRNA precursors that bypass Drosha processing. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ruby%2C+J%2E+Graham%22">Ruby, J. Graham</searchLink><br /><searchLink fieldCode="AR" term="%22Jan%2C+Calvin+H%2E%22">Jan, Calvin H.</searchLink><br /><searchLink fieldCode="AR" term="%22Bartel%2C+David+P%2E%22">Bartel, David P.</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Nature%22">Nature</searchLink>. 7/5/2007, Vol. 448 Issue 7149, p83-86. 4p. 1 Color Photograph, 2 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Ribonucleases%22">Ribonucleases</searchLink><br /><searchLink fieldCode="DE" term="%22Messenger+RNA%22">Messenger RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleotides%22">Nucleotides</searchLink><br /><searchLink fieldCode="DE" term="%22Introns%22">Introns</searchLink><br /><searchLink fieldCode="DE" term="%22Split+genes%22">Split genes</searchLink><br /><searchLink fieldCode="DE" term="%22Drosophila+melanogaster%22">Drosophila melanogaster</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: MicroRNAs (miRNAs) are ∼22-nucleotide endogenous RNAs that often repress the expression of complementary messenger RNAs. In animals, miRNAs derive from characteristic hairpins in primary transcripts through two sequential RNase III-mediated cleavages; Drosha cleaves near the base of the stem to liberate a ∼60-nucleotide pre-miRNA hairpin, then Dicer cleaves near the loop to generate a miRNA:miRNA* duplex. From that duplex, the mature miRNA is incorporated into the silencing complex. Here we identify an alternative pathway for miRNA biogenesis, in which certain debranched introns mimic the structural features of pre-miRNAs to enter the miRNA-processing pathway without Drosha-mediated cleavage. We call these pre-miRNAs/introns ‘mirtrons’, and have identified 14 mirtrons in Drosophila melanogaster and another four in Caenorhabditis elegans (including the reclassification of mir-62). Some of these have been selectively maintained during evolution with patterns of sequence conservation suggesting important regulatory functions in the animal. The abundance of introns comparable in size to pre-miRNAs appears to have created a context favourable for the emergence of mirtrons in flies and nematodes. This suggests that other lineages with many similarly sized introns probably also have mirtrons, and that the mirtron pathway could have provided an early avenue for the emergence of miRNAs before the advent of Drosha. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1038/nature05983 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 4 StartPage: 83 Subjects: – SubjectFull: Ribonucleases Type: general – SubjectFull: Messenger RNA Type: general – SubjectFull: Nucleotides Type: general – SubjectFull: Introns Type: general – SubjectFull: Split genes Type: general – SubjectFull: Drosophila melanogaster Type: general Titles: – TitleFull: Intronic microRNA precursors that bypass Drosha processing. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ruby, J. Graham – PersonEntity: Name: NameFull: Jan, Calvin H. – PersonEntity: Name: NameFull: Bartel, David P. IsPartOfRelationships: – BibEntity: Dates: – D: 05 M: 07 Text: 7/5/2007 Type: published Y: 2007 Identifiers: – Type: issn-print Value: 00280836 Numbering: – Type: volume Value: 448 – Type: issue Value: 7149 Titles: – TitleFull: Nature Type: main |
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