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Expression of MCP-4 by TLR ligand-stimulated nasal polyp fibroblasts.

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Title: Expression of MCP-4 by TLR ligand-stimulated nasal polyp fibroblasts.
Authors: Nonaka, Manabu (AUTHOR), Fukumoto, Akira (AUTHOR), Ogihara, Nozomu (AUTHOR), Pawankar, Ruby (AUTHOR), Sakanushi, Atsuko (AUTHOR), Yagi, Toshiaki (AUTHOR)
Source: Acta Oto-Laryngologica. Dec2007, Vol. 127 Issue 12, p1304-1309. 6p. 2 Graphs.
Subjects: Proteins, Monocytes, Nasal polyps, Fibroblasts, Ligands (Biochemistry), Natural immunity, Eosinophils, Physiology
Abstract: Conclusion. These results indicate that nasal polyp fibroblasts contribute to innate immunity and eosinophilic inflammation such as nasal polyposis. Objective. It is generally accepted that type 2 T helper (Th2) cytokines and some chemoattractants play an essential role in the pathogenesis of nasal polyposis. Nasal polyposis is characterized by chronic eosinophilic inflammation. The mechanisms that cause the predominance of eosinophilic infiltration in nasal polyposis have yet to be clarified. There is growing evidence that fibroblasts could be a major source of Th2 chemokines. Because the nasal and paranasal mucosae are the first respiratory tissues that environmental agents encounter, those tissues are exposed to injurious agents, including microorganisms and their breakdown products. We investigated whether nasal polyp fibroblasts produce a C-C chemokine, MCP-4, when stimulated with the breakdown products of microorganisms and a Th2 cytokine (interleukin (IL)-4). Materials and methods. Fibroblast lines were established from nasal polyp tissues. The expression of MCP-4 mRNA was evaluated by real-time RT-PCR. The amount of MCP-4 in the supernatants was measured by ELISA. Results. TLR2, 3, 4 and 5 ligands, but not TLR7/8 or 9 ligands, induced small amounts of MCP-4. TLR2, 3, 4 and 5 ligands synergized with IL-4 to induce the production of MCP-4. [ABSTRACT FROM AUTHOR]
Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Expression of MCP-4 by TLR ligand-stimulated nasal polyp fibroblasts.
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  Data: <searchLink fieldCode="AR" term="%22Nonaka%2C+Manabu%22">Nonaka, Manabu</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fukumoto%2C+Akira%22">Fukumoto, Akira</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ogihara%2C+Nozomu%22">Ogihara, Nozomu</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pawankar%2C+Ruby%22">Pawankar, Ruby</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sakanushi%2C+Atsuko%22">Sakanushi, Atsuko</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yagi%2C+Toshiaki%22">Yagi, Toshiaki</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Acta+Oto-Laryngologica%22">Acta Oto-Laryngologica</searchLink>. Dec2007, Vol. 127 Issue 12, p1304-1309. 6p. 2 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Monocytes%22">Monocytes</searchLink><br /><searchLink fieldCode="DE" term="%22Nasal+polyps%22">Nasal polyps</searchLink><br /><searchLink fieldCode="DE" term="%22Fibroblasts%22">Fibroblasts</searchLink><br /><searchLink fieldCode="DE" term="%22Ligands+%28Biochemistry%29%22">Ligands (Biochemistry)</searchLink><br /><searchLink fieldCode="DE" term="%22Natural+immunity%22">Natural immunity</searchLink><br /><searchLink fieldCode="DE" term="%22Eosinophils%22">Eosinophils</searchLink><br /><searchLink fieldCode="DE" term="%22Physiology%22">Physiology</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Conclusion. These results indicate that nasal polyp fibroblasts contribute to innate immunity and eosinophilic inflammation such as nasal polyposis. Objective. It is generally accepted that type 2 T helper (Th2) cytokines and some chemoattractants play an essential role in the pathogenesis of nasal polyposis. Nasal polyposis is characterized by chronic eosinophilic inflammation. The mechanisms that cause the predominance of eosinophilic infiltration in nasal polyposis have yet to be clarified. There is growing evidence that fibroblasts could be a major source of Th2 chemokines. Because the nasal and paranasal mucosae are the first respiratory tissues that environmental agents encounter, those tissues are exposed to injurious agents, including microorganisms and their breakdown products. We investigated whether nasal polyp fibroblasts produce a C-C chemokine, MCP-4, when stimulated with the breakdown products of microorganisms and a Th2 cytokine (interleukin (IL)-4). Materials and methods. Fibroblast lines were established from nasal polyp tissues. The expression of MCP-4 mRNA was evaluated by real-time RT-PCR. The amount of MCP-4 in the supernatants was measured by ELISA. Results. TLR2, 3, 4 and 5 ligands, but not TLR7/8 or 9 ligands, induced small amounts of MCP-4. TLR2, 3, 4 and 5 ligands synergized with IL-4 to induce the production of MCP-4. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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        Value: 10.1080/00016480701242444
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      – Code: eng
        Text: English
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        PageCount: 6
        StartPage: 1304
    Subjects:
      – SubjectFull: Proteins
        Type: general
      – SubjectFull: Monocytes
        Type: general
      – SubjectFull: Nasal polyps
        Type: general
      – SubjectFull: Fibroblasts
        Type: general
      – SubjectFull: Ligands (Biochemistry)
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      – SubjectFull: Natural immunity
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      – SubjectFull: Eosinophils
        Type: general
      – SubjectFull: Physiology
        Type: general
    Titles:
      – TitleFull: Expression of MCP-4 by TLR ligand-stimulated nasal polyp fibroblasts.
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            NameFull: Nonaka, Manabu
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            NameFull: Fukumoto, Akira
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            NameFull: Pawankar, Ruby
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            NameFull: Sakanushi, Atsuko
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            – D: 01
              M: 12
              Text: Dec2007
              Type: published
              Y: 2007
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              Value: 127
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