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Keratinocyte growth factor receptor (KGF-R) in cholesteatoma tissue.

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Title: Keratinocyte growth factor receptor (KGF-R) in cholesteatoma tissue.
Authors: Barbara, >M. (AUTHOR), Raffa, S. (AUTHOR), Murè, C. (AUTHOR), Manni, V. (AUTHOR), Ronchetti, F. (AUTHOR), Monini, S. (AUTHOR), Torrisi, M.R. (AUTHOR)
Source: Acta Oto-Laryngologica. Apr2008, Vol. 128 Issue 4, p360-364. 5p. 1 Color Photograph, 1 Chart, 2 Graphs.
Subjects: Keratinocytes, Cholesteatoma, Epithelial tumors, Gene expression, Immunofluorescence
Abstract: Conclusion. Distribution of the receptor for epidermal growth factor (EGF-R) and of the receptor for the keratinocyte growth factor (KGF-R) in cholesteatoma was found to differ in analogy with other epithelial tissues and accordingly to epidermal differentiation and intensity of paracrine stimulation. Moreover, both EGF-R and KGF-R expression was increased, suggesting a fair correlation with aggressiveness and recurrence rate of this pathology. Objectives. To obtain information on the biological behaviour of cholesteatoma by assessing the expression and localization of EGF-R and KGF-R and correlating their tissue distribution with that of cytokeratins as a marker of differentiation. Materials and methods. Cholesteatoma tissue was taken during tympanoplasty surgery and processed for indirect immunofluorescence. Murine monoclonal antibodies were tested for the different growth factor receptors and pancytokeratins analysed. Fluorescence intensity signal was measured on randomly captured digital images, using FISH 2000/HI software, with a pseudocolours generation module. Results. EGF-R was mostly expressed at the level of keratinocytes of the basal layer, while KGF-R signal was mainly distributed on the spinous and granular suprabasal layers that were also highly positive for cytokeratins. Significant correlation between the immunofluorescence signals was found for KGF-R and cytokeratins only, demonstrating that KGF-R expression is increased in more differentiated areas of the cholesteatoma tissue, while EGF-R is associated with proliferative and migratory portions of the lesion. [ABSTRACT FROM AUTHOR]
Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Label: Title
  Group: Ti
  Data: Keratinocyte growth factor receptor (KGF-R) in cholesteatoma tissue.
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  Data: <searchLink fieldCode="AR" term="%22Barbara%2C+>M%2E%22">Barbara, >M.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Raffa%2C+S%2E%22">Raffa, S.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Murè%2C+C%2E%22">Murè, C.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Manni%2C+V%2E%22">Manni, V.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ronchetti%2C+F%2E%22">Ronchetti, F.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Monini%2C+S%2E%22">Monini, S.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Torrisi%2C+M%2ER%2E%22">Torrisi, M.R.</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Acta+Oto-Laryngologica%22">Acta Oto-Laryngologica</searchLink>. Apr2008, Vol. 128 Issue 4, p360-364. 5p. 1 Color Photograph, 1 Chart, 2 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Keratinocytes%22">Keratinocytes</searchLink><br /><searchLink fieldCode="DE" term="%22Cholesteatoma%22">Cholesteatoma</searchLink><br /><searchLink fieldCode="DE" term="%22Epithelial+tumors%22">Epithelial tumors</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Immunofluorescence%22">Immunofluorescence</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Conclusion. Distribution of the receptor for epidermal growth factor (EGF-R) and of the receptor for the keratinocyte growth factor (KGF-R) in cholesteatoma was found to differ in analogy with other epithelial tissues and accordingly to epidermal differentiation and intensity of paracrine stimulation. Moreover, both EGF-R and KGF-R expression was increased, suggesting a fair correlation with aggressiveness and recurrence rate of this pathology. Objectives. To obtain information on the biological behaviour of cholesteatoma by assessing the expression and localization of EGF-R and KGF-R and correlating their tissue distribution with that of cytokeratins as a marker of differentiation. Materials and methods. Cholesteatoma tissue was taken during tympanoplasty surgery and processed for indirect immunofluorescence. Murine monoclonal antibodies were tested for the different growth factor receptors and pancytokeratins analysed. Fluorescence intensity signal was measured on randomly captured digital images, using FISH 2000/HI software, with a pseudocolours generation module. Results. EGF-R was mostly expressed at the level of keratinocytes of the basal layer, while KGF-R signal was mainly distributed on the spinous and granular suprabasal layers that were also highly positive for cytokeratins. Significant correlation between the immunofluorescence signals was found for KGF-R and cytokeratins only, demonstrating that KGF-R expression is increased in more differentiated areas of the cholesteatoma tissue, while EGF-R is associated with proliferative and migratory portions of the lesion. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1080/00016480701785004
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        Text: English
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      – SubjectFull: Keratinocytes
        Type: general
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      – SubjectFull: Epithelial tumors
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      – SubjectFull: Gene expression
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      – SubjectFull: Immunofluorescence
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      – TitleFull: Keratinocyte growth factor receptor (KGF-R) in cholesteatoma tissue.
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              Text: Apr2008
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              Y: 2008
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