Identification of the pollen self-incompatibility determinant in Papaver rhoeas.
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| Title: | Identification of the pollen self-incompatibility determinant in Papaver rhoeas. |
|---|---|
| Authors: | Wheeler, Michael J., de Graaf, Barend H. J., Hadjiosif, Natalie, Perry, Ruth M., Poulter, Natalie S., Osman, Kim, Vatovec, Sabina, Harper, Andrea, Franklin, F. Christopher H., Franklin-Tong, Vernonica E. |
| Source: | Nature. 6/18/2009, Vol. 459 Issue 7249, p992-995. 4p. 2 Diagrams, 1 Graph. |
| Subjects: | Plant self-incompatibility, Pollination, Plant fertilization, Transplantation immunology, Cell membranes, Oligonucleotides, Histocompatibility antigens, Death (Biology), Palynology |
| Abstract: | Higher plants produce seed through pollination, using specific interactions between pollen and pistil. Self-incompatibility is an important mechanism used in many species to prevent inbreeding; it is controlled by a multi-allelic S locus. ‘Self’ (incompatible) pollen is discriminated from ‘non-self’ (compatible) pollen by interaction of pollen and pistil S locus components, and is subsequently inhibited. In Papaver rhoeas, the pistil S locus product is a small protein that interacts with incompatible pollen, triggering a Ca2+-dependent signalling network, resulting in pollen inhibition and programmed cell death. Here we have cloned three alleles of a highly polymorphic pollen-expressed gene, PrpS (Papaver rhoeas pollen S), from Papaver and provide evidence that this encodes the pollen S locus determinant. PrpS is a single-copy gene linked to the pistil S gene (currently called S, but referred to hereafter as PrsS for Papaver rhoeas stigma S determinant). Sequence analysis indicates that PrsS and PrpS are equally ancient and probably co-evolved. PrpS encodes a novel ∼20-kDa protein. Consistent with predictions that it is a transmembrane protein, PrpS is associated with the plasma membrane. We show that a predicted extracellular loop segment of PrpS interacts with PrsS and, using PrpS antisense oligonucleotides, we demonstrate that PrpS is involved in S-specific inhibition of incompatible pollen. Identification of PrpS represents a major advance in our understanding of the Papaver self-incompatibility system. As a novel cell–cell recognition determinant it contributes to the available information concerning the origins and evolution of cell–cell recognition systems involved in discrimination between self and non-self, which also include histocompatibility systems in primitive chordates and vertebrates. [ABSTRACT FROM AUTHOR] |
| Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 41789401 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Identification of the pollen self-incompatibility determinant in Papaver rhoeas. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wheeler%2C+Michael+J%2E%22">Wheeler, Michael J.</searchLink><br /><searchLink fieldCode="AR" term="%22de+Graaf%2C+Barend+H%2E+J%2E%22">de Graaf, Barend H. J.</searchLink><br /><searchLink fieldCode="AR" term="%22Hadjiosif%2C+Natalie%22">Hadjiosif, Natalie</searchLink><br /><searchLink fieldCode="AR" term="%22Perry%2C+Ruth+M%2E%22">Perry, Ruth M.</searchLink><br /><searchLink fieldCode="AR" term="%22Poulter%2C+Natalie+S%2E%22">Poulter, Natalie S.</searchLink><br /><searchLink fieldCode="AR" term="%22Osman%2C+Kim%22">Osman, Kim</searchLink><br /><searchLink fieldCode="AR" term="%22Vatovec%2C+Sabina%22">Vatovec, Sabina</searchLink><br /><searchLink fieldCode="AR" term="%22Harper%2C+Andrea%22">Harper, Andrea</searchLink><br /><searchLink fieldCode="AR" term="%22Franklin%2C+F%2E+Christopher+H%2E%22">Franklin, F. Christopher H.</searchLink><br /><searchLink fieldCode="AR" term="%22Franklin-Tong%2C+Vernonica+E%2E%22">Franklin-Tong, Vernonica E.</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Nature%22">Nature</searchLink>. 6/18/2009, Vol. 459 Issue 7249, p992-995. 4p. 2 Diagrams, 1 Graph. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Plant+self-incompatibility%22">Plant self-incompatibility</searchLink><br /><searchLink fieldCode="DE" term="%22Pollination%22">Pollination</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+fertilization%22">Plant fertilization</searchLink><br /><searchLink fieldCode="DE" term="%22Transplantation+immunology%22">Transplantation immunology</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+membranes%22">Cell membranes</searchLink><br /><searchLink fieldCode="DE" term="%22Oligonucleotides%22">Oligonucleotides</searchLink><br /><searchLink fieldCode="DE" term="%22Histocompatibility+antigens%22">Histocompatibility antigens</searchLink><br /><searchLink fieldCode="DE" term="%22Death+%28Biology%29%22">Death (Biology)</searchLink><br /><searchLink fieldCode="DE" term="%22Palynology%22">Palynology</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Higher plants produce seed through pollination, using specific interactions between pollen and pistil. Self-incompatibility is an important mechanism used in many species to prevent inbreeding; it is controlled by a multi-allelic S locus. ‘Self’ (incompatible) pollen is discriminated from ‘non-self’ (compatible) pollen by interaction of pollen and pistil S locus components, and is subsequently inhibited. In Papaver rhoeas, the pistil S locus product is a small protein that interacts with incompatible pollen, triggering a Ca2+-dependent signalling network, resulting in pollen inhibition and programmed cell death. Here we have cloned three alleles of a highly polymorphic pollen-expressed gene, PrpS (Papaver rhoeas pollen S), from Papaver and provide evidence that this encodes the pollen S locus determinant. PrpS is a single-copy gene linked to the pistil S gene (currently called S, but referred to hereafter as PrsS for Papaver rhoeas stigma S determinant). Sequence analysis indicates that PrsS and PrpS are equally ancient and probably co-evolved. PrpS encodes a novel ∼20-kDa protein. Consistent with predictions that it is a transmembrane protein, PrpS is associated with the plasma membrane. We show that a predicted extracellular loop segment of PrpS interacts with PrsS and, using PrpS antisense oligonucleotides, we demonstrate that PrpS is involved in S-specific inhibition of incompatible pollen. Identification of PrpS represents a major advance in our understanding of the Papaver self-incompatibility system. As a novel cell–cell recognition determinant it contributes to the available information concerning the origins and evolution of cell–cell recognition systems involved in discrimination between self and non-self, which also include histocompatibility systems in primitive chordates and vertebrates. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1038/nature08027 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 4 StartPage: 992 Subjects: – SubjectFull: Plant self-incompatibility Type: general – SubjectFull: Pollination Type: general – SubjectFull: Plant fertilization Type: general – SubjectFull: Transplantation immunology Type: general – SubjectFull: Cell membranes Type: general – SubjectFull: Oligonucleotides Type: general – SubjectFull: Histocompatibility antigens Type: general – SubjectFull: Death (Biology) Type: general – SubjectFull: Palynology Type: general Titles: – TitleFull: Identification of the pollen self-incompatibility determinant in Papaver rhoeas. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wheeler, Michael J. – PersonEntity: Name: NameFull: de Graaf, Barend H. J. – PersonEntity: Name: NameFull: Hadjiosif, Natalie – PersonEntity: Name: NameFull: Perry, Ruth M. – PersonEntity: Name: NameFull: Poulter, Natalie S. – PersonEntity: Name: NameFull: Osman, Kim – PersonEntity: Name: NameFull: Vatovec, Sabina – PersonEntity: Name: NameFull: Harper, Andrea – PersonEntity: Name: NameFull: Franklin, F. Christopher H. – PersonEntity: Name: NameFull: Franklin-Tong, Vernonica E. IsPartOfRelationships: – BibEntity: Dates: – D: 18 M: 06 Text: 6/18/2009 Type: published Y: 2009 Identifiers: – Type: issn-print Value: 00280836 Numbering: – Type: volume Value: 459 – Type: issue Value: 7249 Titles: – TitleFull: Nature Type: main |
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