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Poly(I:C) synergizes with Th2 cytokines to induce TARC/CCL17 in middle ear fibroblasts established from mucosa of otitis media with effusion.

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Title: Poly(I:C) synergizes with Th2 cytokines to induce TARC/CCL17 in middle ear fibroblasts established from mucosa of otitis media with effusion.
Authors: Nonaka, Manabu, Ogihara, Nozomu, Fukumoto, Akira, Sakanushi, Atsuko, Pawankar, Ruby, Yagi, Toshiaki
Source: Acta Oto-Laryngologica (Supplement). Aug2009 Supplement 562, Vol. 129, p57-62. 6p. 4 Graphs.
Subjects: Chemokines, Fibroblasts, Otitis media with effusion, Mucous membranes, Messenger RNA, Gene expression, Reverse transcriptase polymerase chain reaction, Enzyme-linked immunosorbent assay
Abstract: Conclusion: These results suggest that middle ear fibroblasts contribute to the recruitment of Th2 cells into the middle ear by producing thymus and activation-regulated chemokine (TARC). Objectives: Intractable otitis media is more common in atopic subjects and asthmatics than in the otherwise normal population. Although type 2 T helper (Th2) cytokines play crucial roles in the middle ear of these populations, the mechanism underlying the predominance of Th2 cytokines has yet to be clarified. TARC has been known to facilitate recruitment of Th2 polarized cells, resulting in high levels of Th2 cytokines in the middle ear. We investigated whether middle ear-derived fibroblasts produce TARC when stimulated with poly(I:C) and Th2 cytokines (IL-4, IL-13). Materials and methods: Fibroblast lines were established from middle ear mucosa. TARC mRNA expression was evaluated by real-time RT-PCR. The amount of TARC in the culture supernatants was measured by ELISA. Results: Poly(I:C) induced only TARC gene expression in middle ear-derived fibroblasts. Combined stimulation with poly(I:C) and Th2 cytokine (IL-4, IL-13) synergistically induced TARC production by the cultured middle ear-derived fibroblasts. This response was dose and time dependent. [ABSTRACT FROM AUTHOR]
Copyright of Acta Oto-Laryngologica (Supplement) is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Poly(I:C) synergizes with Th2 cytokines to induce TARC/CCL17 in middle ear fibroblasts established from mucosa of otitis media with effusion.
– Name: Author
  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Nonaka%2C+Manabu%22">Nonaka, Manabu</searchLink><br /><searchLink fieldCode="AR" term="%22Ogihara%2C+Nozomu%22">Ogihara, Nozomu</searchLink><br /><searchLink fieldCode="AR" term="%22Fukumoto%2C+Akira%22">Fukumoto, Akira</searchLink><br /><searchLink fieldCode="AR" term="%22Sakanushi%2C+Atsuko%22">Sakanushi, Atsuko</searchLink><br /><searchLink fieldCode="AR" term="%22Pawankar%2C+Ruby%22">Pawankar, Ruby</searchLink><br /><searchLink fieldCode="AR" term="%22Yagi%2C+Toshiaki%22">Yagi, Toshiaki</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Acta+Oto-Laryngologica+%28Supplement%29%22">Acta Oto-Laryngologica (Supplement)</searchLink>. Aug2009 Supplement 562, Vol. 129, p57-62. 6p. 4 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Chemokines%22">Chemokines</searchLink><br /><searchLink fieldCode="DE" term="%22Fibroblasts%22">Fibroblasts</searchLink><br /><searchLink fieldCode="DE" term="%22Otitis+media+with+effusion%22">Otitis media with effusion</searchLink><br /><searchLink fieldCode="DE" term="%22Mucous+membranes%22">Mucous membranes</searchLink><br /><searchLink fieldCode="DE" term="%22Messenger+RNA%22">Messenger RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Reverse+transcriptase+polymerase+chain+reaction%22">Reverse transcriptase polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Enzyme-linked+immunosorbent+assay%22">Enzyme-linked immunosorbent assay</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Conclusion: These results suggest that middle ear fibroblasts contribute to the recruitment of Th2 cells into the middle ear by producing thymus and activation-regulated chemokine (TARC). Objectives: Intractable otitis media is more common in atopic subjects and asthmatics than in the otherwise normal population. Although type 2 T helper (Th2) cytokines play crucial roles in the middle ear of these populations, the mechanism underlying the predominance of Th2 cytokines has yet to be clarified. TARC has been known to facilitate recruitment of Th2 polarized cells, resulting in high levels of Th2 cytokines in the middle ear. We investigated whether middle ear-derived fibroblasts produce TARC when stimulated with poly(I:C) and Th2 cytokines (IL-4, IL-13). Materials and methods: Fibroblast lines were established from middle ear mucosa. TARC mRNA expression was evaluated by real-time RT-PCR. The amount of TARC in the culture supernatants was measured by ELISA. Results: Poly(I:C) induced only TARC gene expression in middle ear-derived fibroblasts. Combined stimulation with poly(I:C) and Th2 cytokine (IL-4, IL-13) synergistically induced TARC production by the cultured middle ear-derived fibroblasts. This response was dose and time dependent. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Acta Oto-Laryngologica (Supplement) is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1080/00016480902911995
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      – Code: eng
        Text: English
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        PageCount: 6
        StartPage: 57
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      – SubjectFull: Chemokines
        Type: general
      – SubjectFull: Fibroblasts
        Type: general
      – SubjectFull: Otitis media with effusion
        Type: general
      – SubjectFull: Mucous membranes
        Type: general
      – SubjectFull: Messenger RNA
        Type: general
      – SubjectFull: Gene expression
        Type: general
      – SubjectFull: Reverse transcriptase polymerase chain reaction
        Type: general
      – SubjectFull: Enzyme-linked immunosorbent assay
        Type: general
    Titles:
      – TitleFull: Poly(I:C) synergizes with Th2 cytokines to induce TARC/CCL17 in middle ear fibroblasts established from mucosa of otitis media with effusion.
        Type: main
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          Name:
            NameFull: Nonaka, Manabu
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            NameFull: Ogihara, Nozomu
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            NameFull: Fukumoto, Akira
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            NameFull: Sakanushi, Atsuko
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            NameFull: Pawankar, Ruby
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            NameFull: Yagi, Toshiaki
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            – D: 01
              M: 08
              Text: Aug2009 Supplement 562
              Type: published
              Y: 2009
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              Value: 03655237
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            – Type: volume
              Value: 129
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            – TitleFull: Acta Oto-Laryngologica (Supplement)
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