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FCHo Proteins Are Nucleators of Clathrin-Mediated Endocytosms.

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Title: FCHo Proteins Are Nucleators of Clathrin-Mediated Endocytosms.
Authors: Henne, William Mike, Boucrot, Emmanuel, Meinecke, Michael, Evergren, Emma, Vallis, Yvonne, Mittal, Rohit, McMahon, Harvey T.
Source: Science (pre-March 2025). 6/4/2010, Vol. 328 Issue 5983, p1281-1284. 4p.
Subjects: Endocytosis, Protein research, Ligands (Chemistry), Eukaryotic cells, Gene expression, Synaptic vesicles
Abstract: Clathrin-mediated endocytosis, the major pathway for ligand internalization into eukaryotic cells, is thought to be initiated by the clustering of clathrin and adaptors around receptors destined for internalization. However, here we report that the membrane-sculpting F-BAR domain-containing Fer/Cip4 homotogy domain-only proteins 1 and 2 (FCHol/2) were required for plasma membrane clathrin-coated vesicle (CCV) budding and marked sites of CCV formation. Changes in FCHol/2 expression levels correlated directly with numbers of CCV budding events, ligand endocytosis, and synaptic vesicle marker recycling. FCHol/2 proteins bound specifically to the plasma membrane and recruited the scaffold proteins epsl5 and intersectin, which in turn engaged the adaptor complex AP2. The FCHo F-BAR membrane-bending activity was required, leading to the proposal that FCHol/2 sculpt the initial bud site and recruit the clathrin machinery for CCV formation. [ABSTRACT FROM AUTHOR]
Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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PubType: Academic Journal
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  Data: FCHo Proteins Are Nucleators of Clathrin-Mediated Endocytosms.
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  Data: <searchLink fieldCode="AR" term="%22Henne%2C+William+Mike%22">Henne, William Mike</searchLink><br /><searchLink fieldCode="AR" term="%22Boucrot%2C+Emmanuel%22">Boucrot, Emmanuel</searchLink><br /><searchLink fieldCode="AR" term="%22Meinecke%2C+Michael%22">Meinecke, Michael</searchLink><br /><searchLink fieldCode="AR" term="%22Evergren%2C+Emma%22">Evergren, Emma</searchLink><br /><searchLink fieldCode="AR" term="%22Vallis%2C+Yvonne%22">Vallis, Yvonne</searchLink><br /><searchLink fieldCode="AR" term="%22Mittal%2C+Rohit%22">Mittal, Rohit</searchLink><br /><searchLink fieldCode="AR" term="%22McMahon%2C+Harvey+T%2E%22">McMahon, Harvey T.</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 6/4/2010, Vol. 328 Issue 5983, p1281-1284. 4p.
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  Data: <searchLink fieldCode="DE" term="%22Endocytosis%22">Endocytosis</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+research%22">Protein research</searchLink><br /><searchLink fieldCode="DE" term="%22Ligands+%28Chemistry%29%22">Ligands (Chemistry)</searchLink><br /><searchLink fieldCode="DE" term="%22Eukaryotic+cells%22">Eukaryotic cells</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Synaptic+vesicles%22">Synaptic vesicles</searchLink>
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  Data: Clathrin-mediated endocytosis, the major pathway for ligand internalization into eukaryotic cells, is thought to be initiated by the clustering of clathrin and adaptors around receptors destined for internalization. However, here we report that the membrane-sculpting F-BAR domain-containing Fer/Cip4 homotogy domain-only proteins 1 and 2 (FCHol/2) were required for plasma membrane clathrin-coated vesicle (CCV) budding and marked sites of CCV formation. Changes in FCHol/2 expression levels correlated directly with numbers of CCV budding events, ligand endocytosis, and synaptic vesicle marker recycling. FCHol/2 proteins bound specifically to the plasma membrane and recruited the scaffold proteins epsl5 and intersectin, which in turn engaged the adaptor complex AP2. The FCHo F-BAR membrane-bending activity was required, leading to the proposal that FCHol/2 sculpt the initial bud site and recruit the clathrin machinery for CCV formation. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1126/science.1188462
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        Text: English
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      – SubjectFull: Protein research
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      – SubjectFull: Ligands (Chemistry)
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      – SubjectFull: Synaptic vesicles
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              Text: 6/4/2010
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              Y: 2010
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