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A modulatory role for protein phosphatase 2B (calcineurin) in the regulation of Ca2+ entry.

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Title: A modulatory role for protein phosphatase 2B (calcineurin) in the regulation of Ca2+ entry.
Authors: Burley, J. Russell (AUTHOR), Sihra, Talvinder S. (AUTHOR)
Source: European Journal of Neuroscience. Aug2000, Vol. 12 Issue 8, p2881-2891. 11p. 1 Chart.
Subjects: Phosphoprotein phosphatases, Calcium channels, Plasmids, Gene transfection, Physiology
Abstract: AbstractThe Ca2+/calmodulin-dependent protein phosphatase 2B (PP2B) also known as calcineurin (CN) has been implicated in the Ca2+-dependent inactivation of Ca2+ channels in several cell types. To study the role of calcineurin in the regulation of Ca2+-channel activity, phosphatase expression was altered in NG108-15 cells by transfection of sense and antisense plasmid constructs carrying the catalytic subunit of human PP2Bβ3. Relative to mock-transfected (wild-type) controls, cells overexpressing calcineurin showed dramatically reduced high-voltage-activated Ca2+ currents which were recoverable by the inclusion of 1 μm FK506 in the patch pipette. Conversely, in cells with reduced calcineurin expression, high-voltage-activated Ca2+ currents were larger relative to controls. Additionally in these cells, low-voltage-activated currents were significantly reduced. Analysis of high-voltage-activated Ca2+ currents revealed that the kinetics of inactivation were significantly accelerated in cells overexpressing calcineurin. Following the delivery of a train of depolarizing pulses in experiments designed to produce large-scale Ca2+ influx across the cell membrane, Ca2+-dependent inactivation of high-voltage-activated Ca2+ currents was increased in sense cells, and this increase could be reduced by intracellular application of 1 mm BAPTA or 1 μm FK506. These data support a role of calcineurin in the negative feedback regulation of Ca2+ entry through voltage-operated Ca2+ channels. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: A modulatory role for protein phosphatase 2B (calcineurin) in the regulation of Ca<superscript>2+</superscript> entry.
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  Data: <searchLink fieldCode="AR" term="%22Burley%2C+J%2E+Russell%22">Burley, J. Russell</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sihra%2C+Talvinder+S%2E%22">Sihra, Talvinder S.</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Aug2000, Vol. 12 Issue 8, p2881-2891. 11p. 1 Chart.
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  Data: <searchLink fieldCode="DE" term="%22Phosphoprotein+phosphatases%22">Phosphoprotein phosphatases</searchLink><br /><searchLink fieldCode="DE" term="%22Calcium+channels%22">Calcium channels</searchLink><br /><searchLink fieldCode="DE" term="%22Plasmids%22">Plasmids</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+transfection%22">Gene transfection</searchLink><br /><searchLink fieldCode="DE" term="%22Physiology%22">Physiology</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: AbstractThe Ca2+/calmodulin-dependent protein phosphatase 2B (PP2B) also known as calcineurin (CN) has been implicated in the Ca2+-dependent inactivation of Ca2+ channels in several cell types. To study the role of calcineurin in the regulation of Ca2+-channel activity, phosphatase expression was altered in NG108-15 cells by transfection of sense and antisense plasmid constructs carrying the catalytic subunit of human PP2Bβ3. Relative to mock-transfected (wild-type) controls, cells overexpressing calcineurin showed dramatically reduced high-voltage-activated Ca2+ currents which were recoverable by the inclusion of 1 μm FK506 in the patch pipette. Conversely, in cells with reduced calcineurin expression, high-voltage-activated Ca2+ currents were larger relative to controls. Additionally in these cells, low-voltage-activated currents were significantly reduced. Analysis of high-voltage-activated Ca2+ currents revealed that the kinetics of inactivation were significantly accelerated in cells overexpressing calcineurin. Following the delivery of a train of depolarizing pulses in experiments designed to produce large-scale Ca2+ influx across the cell membrane, Ca2+-dependent inactivation of high-voltage-activated Ca2+ currents was increased in sense cells, and this increase could be reduced by intracellular application of 1 mm BAPTA or 1 μm FK506. These data support a role of calcineurin in the negative feedback regulation of Ca2+ entry through voltage-operated Ca2+ channels. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Group: Ab
  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1046/j.1460-9568.2000.00178.x
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      – Code: eng
        Text: English
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        PageCount: 11
        StartPage: 2881
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      – SubjectFull: Phosphoprotein phosphatases
        Type: general
      – SubjectFull: Calcium channels
        Type: general
      – SubjectFull: Plasmids
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      – SubjectFull: Gene transfection
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      – TitleFull: A modulatory role for protein phosphatase 2B (calcineurin) in the regulation of Ca2+ entry.
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              M: 08
              Text: Aug2000
              Type: published
              Y: 2000
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