View in EDS HTML Full Text PDF Full Text

Inflammatory cytokines and related genes are induced in the rat hippocampus by limbic status epilepticus.

Saved in:
Bibliographic Details
Title: Inflammatory cytokines and related genes are induced in the rat hippocampus by limbic status epilepticus.
Authors: De Simoni, Maria Grazia (AUTHOR), Perego, Carlo (AUTHOR), Ravizza, Teresa (AUTHOR), Moneta, Daniela (AUTHOR), Conti, Mirko (AUTHOR), Marchesi, Francesco (AUTHOR), De Luigi, Ada (AUTHOR), Garattini, Silvio (AUTHOR), Vezzani, Annamaria (AUTHOR)
Source: European Journal of Neuroscience. Jul2000, Vol. 12 Issue 7, p2623-2633. 11p. 2 Charts.
Subjects: Cytokines, Hippocampus (Brain)
Abstract: AbstractLimbic status epilepticus was induced in rats by unilateral 60-min electrical stimulation of the CA3 region of the ventral hippocampus. As assessed by RT-PCR followed by Southern blot analysis, transcripts of interleukin-1β, interleukin-6, interleukin-1 receptor antagonist and inducible nitric oxide synthase were significantly increased 2 h after status epilepticus in the stimulated hippocampus. Induction was maximal at 6 h for interleukin-1β (445%), interleukin-6 (405%) and tumour necrosis factor-α (264%) and at 24 h for interleukin-1 receptor antagonist (494%) and inducible nitric oxide synthase (432%). In rats with spontaneous seizures (60 days after status epilepticus), interleukin-1β mRNA was still higher than controls (241%). Immunocytochemical staining of interleukin-1β, interleukin-6 and tumour necrosis factor-α was enhanced in glia with a time-course similar to that of the respective transcripts. Sixty days after status epilepticus, interleukin-1β immunoreactivity was increased exclusively in neurons in one third of the animals. Multiple intracerebroventricular injections of interleukin-1 receptor antagonist (0.5 μg/3 μL) significantly decreased the severity of behavioural convulsions during electrical stimulation and selectively reduced tumour necrosis factor-α content in the hippocampus measured 18 h after status epilepticus. Thus, the induction of spontaneously recurring seizures in rats involves the activation of inflammatory cytokines and related pro- and anti-inflammatory genes in the hippocampus. These changes may play an active role in hyperexcitability of the epileptic tissue. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: pbh
DbLabel: Psychology and Behavioral Sciences Collection
An: 5184846
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Inflammatory cytokines and related genes are induced in the rat hippocampus by limbic status epilepticus.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22De+Simoni%2C+Maria+Grazia%22">De Simoni, Maria Grazia</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Perego%2C+Carlo%22">Perego, Carlo</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ravizza%2C+Teresa%22">Ravizza, Teresa</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Moneta%2C+Daniela%22">Moneta, Daniela</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Conti%2C+Mirko%22">Conti, Mirko</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Marchesi%2C+Francesco%22">Marchesi, Francesco</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22De+Luigi%2C+Ada%22">De Luigi, Ada</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Garattini%2C+Silvio%22">Garattini, Silvio</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vezzani%2C+Annamaria%22">Vezzani, Annamaria</searchLink> (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Jul2000, Vol. 12 Issue 7, p2623-2633. 11p. 2 Charts.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Cytokines%22">Cytokines</searchLink><br /><searchLink fieldCode="DE" term="%22Hippocampus+%28Brain%29%22">Hippocampus (Brain)</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: AbstractLimbic status epilepticus was induced in rats by unilateral 60-min electrical stimulation of the CA3 region of the ventral hippocampus. As assessed by RT-PCR followed by Southern blot analysis, transcripts of interleukin-1β, interleukin-6, interleukin-1 receptor antagonist and inducible nitric oxide synthase were significantly increased 2 h after status epilepticus in the stimulated hippocampus. Induction was maximal at 6 h for interleukin-1β (445%), interleukin-6 (405%) and tumour necrosis factor-α (264%) and at 24 h for interleukin-1 receptor antagonist (494%) and inducible nitric oxide synthase (432%). In rats with spontaneous seizures (60 days after status epilepticus), interleukin-1β mRNA was still higher than controls (241%). Immunocytochemical staining of interleukin-1β, interleukin-6 and tumour necrosis factor-α was enhanced in glia with a time-course similar to that of the respective transcripts. Sixty days after status epilepticus, interleukin-1β immunoreactivity was increased exclusively in neurons in one third of the animals. Multiple intracerebroventricular injections of interleukin-1 receptor antagonist (0.5 μg/3 μL) significantly decreased the severity of behavioural convulsions during electrical stimulation and selectively reduced tumour necrosis factor-α content in the hippocampus measured 18 h after status epilepticus. Thus, the induction of spontaneously recurring seizures in rats involves the activation of inflammatory cytokines and related pro- and anti-inflammatory genes in the hippocampus. These changes may play an active role in hyperexcitability of the epileptic tissue. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=5184846
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1046/j.1460-9568.2000.00140.x
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 11
        StartPage: 2623
    Subjects:
      – SubjectFull: Cytokines
        Type: general
      – SubjectFull: Hippocampus (Brain)
        Type: general
    Titles:
      – TitleFull: Inflammatory cytokines and related genes are induced in the rat hippocampus by limbic status epilepticus.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: De Simoni, Maria Grazia
      – PersonEntity:
          Name:
            NameFull: Perego, Carlo
      – PersonEntity:
          Name:
            NameFull: Ravizza, Teresa
      – PersonEntity:
          Name:
            NameFull: Moneta, Daniela
      – PersonEntity:
          Name:
            NameFull: Conti, Mirko
      – PersonEntity:
          Name:
            NameFull: Marchesi, Francesco
      – PersonEntity:
          Name:
            NameFull: De Luigi, Ada
      – PersonEntity:
          Name:
            NameFull: Garattini, Silvio
      – PersonEntity:
          Name:
            NameFull: Vezzani, Annamaria
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 07
              Text: Jul2000
              Type: published
              Y: 2000
          Identifiers:
            – Type: issn-print
              Value: 0953816X
          Numbering:
            – Type: volume
              Value: 12
            – Type: issue
              Value: 7
          Titles:
            – TitleFull: European Journal of Neuroscience
              Type: main
ResultId 1