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A novel P0 glycoprotein transgene activates expression of lacZ in myelin-forming Schwann cells.

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Title: A novel P0 glycoprotein transgene activates expression of lacZ in myelin-forming Schwann cells.
Authors: Feltri, M. Laura (AUTHOR), D'Antonio, Maurizio (AUTHOR), Quattrini, Angelo (AUTHOR), Numerato, Rita (AUTHOR), Arona, Marta (AUTHOR), Previtali, Stefano (AUTHOR), Chiu, Shing‐Yan (AUTHOR), Messing, Albee (AUTHOR), Wrabetz, Lawrence (AUTHOR)
Source: European Journal of Neuroscience. May99, Vol. 11 Issue 5, p1577-1586. 10p. 1 Chart.
Subjects: Beta-galactosidase, Transgenic mice, Axons, Myelin sheath
Abstract: Abstract P0 glycoprotein, the most abundant protein in peripheral nerve, is expressed specifically in the Schwann cell lineage. Upstream of the rat P0 gene 1.1 kb of DNA can activate expression of cDNAs specifically in Schwann cells in transgenic mice. However, the expression of P0 promoter-based transgenes has been inconsistent. As much as 9 kb of 5′ flanking sequence fused to lacZ never yielded detectable levels of β-galactosidase in multiple lines of mice. We describe transgenic mice that express lacZ in peripheral nerve, using the complete mouse P0 gene, including 6 kb of 5′ flanking sequence, all exons and introns, and the natural polyadenylation signal. This vector activated lacZ expression specifically in cultured Schwann cells, and myelin-forming Schwann cells in four out of six transgenic lines. Transgene expression paralleled that of the endogenous P0 gene, both during development and after Wallerian degeneration. lacZ expression was lower than endogenous P0 expression, and was not detected in neural crest or Schwann cell precursors, where low levels of P0 mRNA are present. However, when the same vector contained a small myc tag instead of the 3.2-kb lacZ insert, the resulting transgenic mRNA was expressed at levels comparable to endogenous P0 mRNA. These data suggest that intragenic or 3′ flanking sequences are necessary to generate the remarkable levels of endogenous P0 gene expression. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Label: Title
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  Data: A novel P<subscript>0</subscript> glycoprotein transgene activates expression of lacZ in myelin-forming Schwann cells.
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  Data: <searchLink fieldCode="AR" term="%22Feltri%2C+M%2E+Laura%22">Feltri, M. Laura</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22D'Antonio%2C+Maurizio%22">D'Antonio, Maurizio</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Quattrini%2C+Angelo%22">Quattrini, Angelo</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Numerato%2C+Rita%22">Numerato, Rita</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Arona%2C+Marta%22">Arona, Marta</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Previtali%2C+Stefano%22">Previtali, Stefano</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chiu%2C+Shing‐Yan%22">Chiu, Shing‐Yan</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Messing%2C+Albee%22">Messing, Albee</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wrabetz%2C+Lawrence%22">Wrabetz, Lawrence</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. May99, Vol. 11 Issue 5, p1577-1586. 10p. 1 Chart.
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  Data: <searchLink fieldCode="DE" term="%22Beta-galactosidase%22">Beta-galactosidase</searchLink><br /><searchLink fieldCode="DE" term="%22Transgenic+mice%22">Transgenic mice</searchLink><br /><searchLink fieldCode="DE" term="%22Axons%22">Axons</searchLink><br /><searchLink fieldCode="DE" term="%22Myelin+sheath%22">Myelin sheath</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Abstract P0 glycoprotein, the most abundant protein in peripheral nerve, is expressed specifically in the Schwann cell lineage. Upstream of the rat P0 gene 1.1 kb of DNA can activate expression of cDNAs specifically in Schwann cells in transgenic mice. However, the expression of P0 promoter-based transgenes has been inconsistent. As much as 9 kb of 5′ flanking sequence fused to lacZ never yielded detectable levels of β-galactosidase in multiple lines of mice. We describe transgenic mice that express lacZ in peripheral nerve, using the complete mouse P0 gene, including 6 kb of 5′ flanking sequence, all exons and introns, and the natural polyadenylation signal. This vector activated lacZ expression specifically in cultured Schwann cells, and myelin-forming Schwann cells in four out of six transgenic lines. Transgene expression paralleled that of the endogenous P0 gene, both during development and after Wallerian degeneration. lacZ expression was lower than endogenous P0 expression, and was not detected in neural crest or Schwann cell precursors, where low levels of P0 mRNA are present. However, when the same vector contained a small myc tag instead of the 3.2-kb lacZ insert, the resulting transgenic mRNA was expressed at levels comparable to endogenous P0 mRNA. These data suggest that intragenic or 3′ flanking sequences are necessary to generate the remarkable levels of endogenous P0 gene expression. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1046/j.1460-9568.1999.00568.x
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      – Code: eng
        Text: English
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        Type: general
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      – SubjectFull: Axons
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      – SubjectFull: Myelin sheath
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              M: 05
              Text: May99
              Type: published
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