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Molecular cloning of the Coch gene of guinea pig inner ear and its expression analysis in cultured fibrocytes of the spiral ligament.

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Title: Molecular cloning of the Coch gene of guinea pig inner ear and its expression analysis in cultured fibrocytes of the spiral ligament.
Authors: Li, Lishu (AUTHOR), Ikezono, Tetsuo (AUTHOR), Sekine, Kuwon (AUTHOR), Shindo, Susumu (AUTHOR), Matsumura, Tomohiro (AUTHOR), Pawankar, Ruby (AUTHOR), Ichimiya, Issei (AUTHOR), Yagi, Toshiaki (AUTHOR)
Source: Acta Oto-Laryngologica. Aug2010, Vol. 130 Issue 8, p868-880. 13p. 1 Color Photograph, 2 Charts.
Subjects: Molecular cloning, Gene expression, Inner ear, Fibroblasts, Cytokines, Guinea pigs as laboratory animals
Abstract: Conclusions: We have cloned guinea pig Coch cDNA and the sequence information will be useful for future molecular study combined with physiological experiments. Proper Coch gene expression appears to be dependent on the unique extracellular micro-environment of the inner ear in vivo. These results provide insight into the Coch gene expression and its regulation. Objective: To characterize the guinea pig Coch gene, we performed molecular cloning and expression analysis in the inner ear and cultured fibrocytes of the spiral ligament. Methods: The Coch cDNA was isolated using RACE. Cochlin isofoms were studied by Western blot using three different types of mammalian inner ear. The cochlear fibrocytes were cultured and characterized by immunostaining. Coch gene expression in the fibrocytes was investigated and the influence of cytokine stimulation was evaluated. Results: The full-length 1991 bp Coch cDNA that encodes a 553 amino acid protein was isolated. The sequence had significant homology with other mammals, and the sizes of the Cochlin isoforms were identical. In the cultured fibrocytes, Coch mRNA was expressed in a very small amount and the isoform production was different, compared with the results in vivo. Cytokine stimulation did not alter the level of mRNA expression or isoform formation. [ABSTRACT FROM AUTHOR]
Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Label: Title
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  Data: Molecular cloning of the Coch gene of guinea pig inner ear and its expression analysis in cultured fibrocytes of the spiral ligament.
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  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Li%2C+Lishu%22">Li, Lishu</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ikezono%2C+Tetsuo%22">Ikezono, Tetsuo</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sekine%2C+Kuwon%22">Sekine, Kuwon</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shindo%2C+Susumu%22">Shindo, Susumu</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Matsumura%2C+Tomohiro%22">Matsumura, Tomohiro</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pawankar%2C+Ruby%22">Pawankar, Ruby</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ichimiya%2C+Issei%22">Ichimiya, Issei</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yagi%2C+Toshiaki%22">Yagi, Toshiaki</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Acta+Oto-Laryngologica%22">Acta Oto-Laryngologica</searchLink>. Aug2010, Vol. 130 Issue 8, p868-880. 13p. 1 Color Photograph, 2 Charts.
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  Label: Subjects
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  Data: <searchLink fieldCode="DE" term="%22Molecular+cloning%22">Molecular cloning</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Inner+ear%22">Inner ear</searchLink><br /><searchLink fieldCode="DE" term="%22Fibroblasts%22">Fibroblasts</searchLink><br /><searchLink fieldCode="DE" term="%22Cytokines%22">Cytokines</searchLink><br /><searchLink fieldCode="DE" term="%22Guinea+pigs+as+laboratory+animals%22">Guinea pigs as laboratory animals</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Conclusions: We have cloned guinea pig Coch cDNA and the sequence information will be useful for future molecular study combined with physiological experiments. Proper Coch gene expression appears to be dependent on the unique extracellular micro-environment of the inner ear in vivo. These results provide insight into the Coch gene expression and its regulation. Objective: To characterize the guinea pig Coch gene, we performed molecular cloning and expression analysis in the inner ear and cultured fibrocytes of the spiral ligament. Methods: The Coch cDNA was isolated using RACE. Cochlin isofoms were studied by Western blot using three different types of mammalian inner ear. The cochlear fibrocytes were cultured and characterized by immunostaining. Coch gene expression in the fibrocytes was investigated and the influence of cytokine stimulation was evaluated. Results: The full-length 1991 bp Coch cDNA that encodes a 553 amino acid protein was isolated. The sequence had significant homology with other mammals, and the sizes of the Cochlin isoforms were identical. In the cultured fibrocytes, Coch mRNA was expressed in a very small amount and the isoform production was different, compared with the results in vivo. Cytokine stimulation did not alter the level of mRNA expression or isoform formation. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3109/00016480903493766
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      – Code: eng
        Text: English
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        PageCount: 13
        StartPage: 868
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      – SubjectFull: Molecular cloning
        Type: general
      – SubjectFull: Gene expression
        Type: general
      – SubjectFull: Inner ear
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      – SubjectFull: Fibroblasts
        Type: general
      – SubjectFull: Cytokines
        Type: general
      – SubjectFull: Guinea pigs as laboratory animals
        Type: general
    Titles:
      – TitleFull: Molecular cloning of the Coch gene of guinea pig inner ear and its expression analysis in cultured fibrocytes of the spiral ligament.
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            NameFull: Li, Lishu
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              M: 08
              Text: Aug2010
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              Y: 2010
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