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Reversible Microbial Colonization of Germ-Free Mice Reveals the Dynamics of IgA Immune Responses.

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Title: Reversible Microbial Colonization of Germ-Free Mice Reveals the Dynamics of IgA Immune Responses.
Authors: Hapfelmeier, Siegfried, Lawson, Melissa A. E., Slack, Emma, Kirundi, Jorum K., Stoel, Maaike, Heikenwalder, Mathias, Cahenzli, Julia, Velykoredko, Yuliya, Balmer, Maria L., Endt, Kathrin, Geuking, Markus B., Curtiss 3rd, Roy, McCoy, Kathy D., Macpherson, Andrew J.
Source: Science (pre-March 2025). 6/25/2010, Vol. 328 Issue 5986, p1705-1709. 5p.
Subjects: Large intestine diseases, Commensalism, Bacteria, Microbiology, Immune response, Immunoglobulin A, Laboratory mice, Vaccination
Abstract: The lower intestine of adult mammals is densely colonized with nonpathogenic (commensal) microbes. Gut bacteria induce protective immune responses, which ensure host-microbial mutualism. The continuous presence of commensal intestinal bacteria has made it difficult to study mucosal immune dynamics. Here, we report a reversible germ-free colonization system in mice that is independent of diet or antibiotic manipulation. A slow (more than 14 days) onset of a long-lived (half-life over 16 weeks), highly specific anticommensal immunoglobulin A (IgA) response in germ-free mice was observed. Ongoing commensal exposure in colonized mice rapidly abrogated this response. Sequential doses lacked a classical prime-boost effect seen in systemic vaccination, but specific IgA induction occurred as a stepwise response to current bacterial exposure, such that the antibody repertoire matched the existing commensal content. [ABSTRACT FROM AUTHOR]
Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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PubType: Academic Journal
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  Data: Reversible Microbial Colonization of Germ-Free Mice Reveals the Dynamics of IgA Immune Responses.
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  Data: <searchLink fieldCode="AR" term="%22Hapfelmeier%2C+Siegfried%22">Hapfelmeier, Siegfried</searchLink><br /><searchLink fieldCode="AR" term="%22Lawson%2C+Melissa+A%2E+E%2E%22">Lawson, Melissa A. E.</searchLink><br /><searchLink fieldCode="AR" term="%22Slack%2C+Emma%22">Slack, Emma</searchLink><br /><searchLink fieldCode="AR" term="%22Kirundi%2C+Jorum+K%2E%22">Kirundi, Jorum K.</searchLink><br /><searchLink fieldCode="AR" term="%22Stoel%2C+Maaike%22">Stoel, Maaike</searchLink><br /><searchLink fieldCode="AR" term="%22Heikenwalder%2C+Mathias%22">Heikenwalder, Mathias</searchLink><br /><searchLink fieldCode="AR" term="%22Cahenzli%2C+Julia%22">Cahenzli, Julia</searchLink><br /><searchLink fieldCode="AR" term="%22Velykoredko%2C+Yuliya%22">Velykoredko, Yuliya</searchLink><br /><searchLink fieldCode="AR" term="%22Balmer%2C+Maria+L%2E%22">Balmer, Maria L.</searchLink><br /><searchLink fieldCode="AR" term="%22Endt%2C+Kathrin%22">Endt, Kathrin</searchLink><br /><searchLink fieldCode="AR" term="%22Geuking%2C+Markus+B%2E%22">Geuking, Markus B.</searchLink><br /><searchLink fieldCode="AR" term="%22Curtiss+3rd%2C+Roy%22">Curtiss 3rd, Roy</searchLink><br /><searchLink fieldCode="AR" term="%22McCoy%2C+Kathy+D%2E%22">McCoy, Kathy D.</searchLink><br /><searchLink fieldCode="AR" term="%22Macpherson%2C+Andrew+J%2E%22">Macpherson, Andrew J.</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 6/25/2010, Vol. 328 Issue 5986, p1705-1709. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Large+intestine+diseases%22">Large intestine diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Commensalism%22">Commensalism</searchLink><br /><searchLink fieldCode="DE" term="%22Bacteria%22">Bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Microbiology%22">Microbiology</searchLink><br /><searchLink fieldCode="DE" term="%22Immune+response%22">Immune response</searchLink><br /><searchLink fieldCode="DE" term="%22Immunoglobulin+A%22">Immunoglobulin A</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+mice%22">Laboratory mice</searchLink><br /><searchLink fieldCode="DE" term="%22Vaccination%22">Vaccination</searchLink>
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  Label: Abstract
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  Data: The lower intestine of adult mammals is densely colonized with nonpathogenic (commensal) microbes. Gut bacteria induce protective immune responses, which ensure host-microbial mutualism. The continuous presence of commensal intestinal bacteria has made it difficult to study mucosal immune dynamics. Here, we report a reversible germ-free colonization system in mice that is independent of diet or antibiotic manipulation. A slow (more than 14 days) onset of a long-lived (half-life over 16 weeks), highly specific anticommensal immunoglobulin A (IgA) response in germ-free mice was observed. Ongoing commensal exposure in colonized mice rapidly abrogated this response. Sequential doses lacked a classical prime-boost effect seen in systemic vaccination, but specific IgA induction occurred as a stepwise response to current bacterial exposure, such that the antibody repertoire matched the existing commensal content. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=52367069
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        Value: 10.1126/science.1188454
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        Text: English
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        PageCount: 5
        StartPage: 1705
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      – SubjectFull: Large intestine diseases
        Type: general
      – SubjectFull: Commensalism
        Type: general
      – SubjectFull: Bacteria
        Type: general
      – SubjectFull: Microbiology
        Type: general
      – SubjectFull: Immune response
        Type: general
      – SubjectFull: Immunoglobulin A
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      – SubjectFull: Laboratory mice
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      – SubjectFull: Vaccination
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      – TitleFull: Reversible Microbial Colonization of Germ-Free Mice Reveals the Dynamics of IgA Immune Responses.
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              Text: 6/25/2010
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