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Glutamate receptor activation can trigger electrical activity in human glioma cells.

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Title: Glutamate receptor activation can trigger electrical activity in human glioma cells.
Authors: Labrakakis, Charalampos (AUTHOR), Patt, Stephan (AUTHOR), Hartmann, Jana (AUTHOR), Kettenmann, Helmut (AUTHOR)
Source: European Journal of Neuroscience. Jun98, Vol. 10 Issue 6-2162.
Subjects: Glutamine, Gliomas, Patch-clamp techniques (Electrophysiology)
Abstract: Cells from major types of gliomas, i.e. oligodendrogliomas and glioblastomas, are able to generate action potentials upon a current injection similar to neurons (Patt et al. (1996) Neuroscience, 71, 601–611; Labrakakis et al. (1997b) J. Neuropath. Exp. Neurol., 56, 243–254. Here, we report that activation of ionotropic glutamate receptors by the selective agonist, kainate, or by glutamate itself, depolarized the tumour cells in culture and living slices from tumour tissue, and can elicit volleys of action potentials, as recorded with the patch-clamp technique. Sixty-six percent of the glioblastoma cells, 44% of the astocytoma and 86% of the oligodendroglioma cells responded to glutamate and the specific agonist of AMPA/kainate receptors, kainate. The involvement of non-NMDA (N-methyl-d-aspartate) receptors is further supported by the observation that both kainate and glutamate currents were blocked by CNQX (6-cyano-7-nitroquinoxaline-2,3-dione). The receptor activation was accompanied by an increase in cytosolic Ca[sup 2+], as recorded with a fura-2 microfluorometric system. The Ca[sup 2+] elevation was mediated by the activation of Ca[sup 2+] channels due to membrane depolarization. The presence of voltage-gated Ca[sup 2+] channels was confirmed by patch-clamp experiments. Taken together, these findings imply that the electrophysiological properties of glioma cells are more reminiscent of those of neurons than of glial cells. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Glutamate receptor activation can trigger electrical activity in human glioma cells.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Labrakakis%2C+Charalampos%22">Labrakakis, Charalampos</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Patt%2C+Stephan%22">Patt, Stephan</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hartmann%2C+Jana%22">Hartmann, Jana</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kettenmann%2C+Helmut%22">Kettenmann, Helmut</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Jun98, Vol. 10 Issue 6-2162.
– Name: Subject
  Label: Subjects
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  Data: <searchLink fieldCode="DE" term="%22Glutamine%22">Glutamine</searchLink><br /><searchLink fieldCode="DE" term="%22Gliomas%22">Gliomas</searchLink><br /><searchLink fieldCode="DE" term="%22Patch-clamp+techniques+%28Electrophysiology%29%22">Patch-clamp techniques (Electrophysiology)</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Cells from major types of gliomas, i.e. oligodendrogliomas and glioblastomas, are able to generate action potentials upon a current injection similar to neurons (Patt et al. (1996) Neuroscience, 71, 601–611; Labrakakis et al. (1997b) J. Neuropath. Exp. Neurol., 56, 243–254. Here, we report that activation of ionotropic glutamate receptors by the selective agonist, kainate, or by glutamate itself, depolarized the tumour cells in culture and living slices from tumour tissue, and can elicit volleys of action potentials, as recorded with the patch-clamp technique. Sixty-six percent of the glioblastoma cells, 44% of the astocytoma and 86% of the oligodendroglioma cells responded to glutamate and the specific agonist of AMPA/kainate receptors, kainate. The involvement of non-NMDA (N-methyl-d-aspartate) receptors is further supported by the observation that both kainate and glutamate currents were blocked by CNQX (6-cyano-7-nitroquinoxaline-2,3-dione). The receptor activation was accompanied by an increase in cytosolic Ca[sup 2+], as recorded with a fura-2 microfluorometric system. The Ca[sup 2+] elevation was mediated by the activation of Ca[sup 2+] channels due to membrane depolarization. The presence of voltage-gated Ca[sup 2+] channels was confirmed by patch-clamp experiments. Taken together, these findings imply that the electrophysiological properties of glioma cells are more reminiscent of those of neurons than of glial cells. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1046/j.1460-9568.1998.00226.x
    Languages:
      – Code: eng
        Text: English
    Subjects:
      – SubjectFull: Glutamine
        Type: general
      – SubjectFull: Gliomas
        Type: general
      – SubjectFull: Patch-clamp techniques (Electrophysiology)
        Type: general
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      – TitleFull: Glutamate receptor activation can trigger electrical activity in human glioma cells.
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            NameFull: Labrakakis, Charalampos
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            NameFull: Patt, Stephan
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            NameFull: Hartmann, Jana
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            NameFull: Kettenmann, Helmut
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            – D: 01
              M: 06
              Text: Jun98
              Type: published
              Y: 1998
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              Value: 0953816X
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              Value: 10
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            – TitleFull: European Journal of Neuroscience
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