Effect of Roxithromycin on IL-8 Synthesis and Proliferation of Nasal Polyp Fibroblasts.
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| Title: | Effect of Roxithromycin on IL-8 Synthesis and Proliferation of Nasal Polyp Fibroblasts. |
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| Authors: | Nonaka, Manabu, Pawankar, Ruby, Saji, Fusako, Yagi, Toshiaki |
| Source: | Acta Oto-Laryngologica (Supplement). Nov1998 Supplement 539, Vol. 118, p71-75. 5p. |
| Subjects: | Macrolide antibiotics, Interleukin-8, Cell proliferation, Fibroblasts, Nasal polyps, Physiology, Biosynthesis |
| Abstract: | Although several studies have demonstrated that low-dose, long-term 14-member macrolides (erythromycin (EM), roxithromycin (RXM), clarithromycin (CAM)) are effective in the treatment of chronic airway diseases like chronic sinusitis and diffuse panbronchiolitis (DPB), the mechanism of action of these drugs is not yet clear. Both these airway diseases are associated with an increase in the proliferation of fibroblasts. Moreover, fibroblasts are also an important source of proinflammatory cytokines such as interleukin-8 (IL-8), that play an important role in the pathogenesis of nasal polyps. Therefore, using primary fibroblast lines derived from nasal polyps, we investigated the effect of RXM on the synthesis of IL-8 and proliferation of nasal polyp fibroblasts (NPF). These fibroblasts were either treated with lipopolysaccharide (LPS) and RXM for 24 h, or pre-incubated with RXM for 24 h and then treated with LPS and RXM for 24 h. The level of IL-8 mRNA in NPF was analysed by reverse transcriptase-polymerase chain (RT-PCR) and the level of IL-8 in culture supernatants was measured by ELISA. Next, the proliferative capacity of NPF after treatment with RXM was analysed by cell counting and [sup 3]H-thymidine uptake. RXM had no effect on LPS-induced IL-8 synthesis by NPF. On the other hand, RXM suppressed the proliferation of NPF in a dose-dependent manner. These findings suggest that, although RXM cannot directly inhibit the synthesis of IL-8, it probably reduces IL-8 production by inhibiting the proliferation of NPF. [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Oto-Laryngologica (Supplement) is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 5323223 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Effect of Roxithromycin on IL-8 Synthesis and Proliferation of Nasal Polyp Fibroblasts. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Nonaka%2C+Manabu%22">Nonaka, Manabu</searchLink><br /><searchLink fieldCode="AR" term="%22Pawankar%2C+Ruby%22">Pawankar, Ruby</searchLink><br /><searchLink fieldCode="AR" term="%22Saji%2C+Fusako%22">Saji, Fusako</searchLink><br /><searchLink fieldCode="AR" term="%22Yagi%2C+Toshiaki%22">Yagi, Toshiaki</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Acta+Oto-Laryngologica+%28Supplement%29%22">Acta Oto-Laryngologica (Supplement)</searchLink>. Nov1998 Supplement 539, Vol. 118, p71-75. 5p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Macrolide+antibiotics%22">Macrolide antibiotics</searchLink><br /><searchLink fieldCode="DE" term="%22Interleukin-8%22">Interleukin-8</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+proliferation%22">Cell proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22Fibroblasts%22">Fibroblasts</searchLink><br /><searchLink fieldCode="DE" term="%22Nasal+polyps%22">Nasal polyps</searchLink><br /><searchLink fieldCode="DE" term="%22Physiology%22">Physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Biosynthesis%22">Biosynthesis</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Although several studies have demonstrated that low-dose, long-term 14-member macrolides (erythromycin (EM), roxithromycin (RXM), clarithromycin (CAM)) are effective in the treatment of chronic airway diseases like chronic sinusitis and diffuse panbronchiolitis (DPB), the mechanism of action of these drugs is not yet clear. Both these airway diseases are associated with an increase in the proliferation of fibroblasts. Moreover, fibroblasts are also an important source of proinflammatory cytokines such as interleukin-8 (IL-8), that play an important role in the pathogenesis of nasal polyps. Therefore, using primary fibroblast lines derived from nasal polyps, we investigated the effect of RXM on the synthesis of IL-8 and proliferation of nasal polyp fibroblasts (NPF). These fibroblasts were either treated with lipopolysaccharide (LPS) and RXM for 24 h, or pre-incubated with RXM for 24 h and then treated with LPS and RXM for 24 h. The level of IL-8 mRNA in NPF was analysed by reverse transcriptase-polymerase chain (RT-PCR) and the level of IL-8 in culture supernatants was measured by ELISA. Next, the proliferative capacity of NPF after treatment with RXM was analysed by cell counting and [sup 3]H-thymidine uptake. RXM had no effect on LPS-induced IL-8 synthesis by NPF. On the other hand, RXM suppressed the proliferation of NPF in a dose-dependent manner. These findings suggest that, although RXM cannot directly inhibit the synthesis of IL-8, it probably reduces IL-8 production by inhibiting the proliferation of NPF. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Acta Oto-Laryngologica (Supplement) is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1080/00016489850182170 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 5 StartPage: 71 Subjects: – SubjectFull: Macrolide antibiotics Type: general – SubjectFull: Interleukin-8 Type: general – SubjectFull: Cell proliferation Type: general – SubjectFull: Fibroblasts Type: general – SubjectFull: Nasal polyps Type: general – SubjectFull: Physiology Type: general – SubjectFull: Biosynthesis Type: general Titles: – TitleFull: Effect of Roxithromycin on IL-8 Synthesis and Proliferation of Nasal Polyp Fibroblasts. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Nonaka, Manabu – PersonEntity: Name: NameFull: Pawankar, Ruby – PersonEntity: Name: NameFull: Saji, Fusako – PersonEntity: Name: NameFull: Yagi, Toshiaki IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 11 Text: Nov1998 Supplement 539 Type: published Y: 1998 Identifiers: – Type: issn-print Value: 03655237 Numbering: – Type: volume Value: 118 Titles: – TitleFull: Acta Oto-Laryngologica (Supplement) Type: main |
| ResultId | 1 |
