Intercellular Adhesion Molecule-1 Expression in the Inner Ear of Rats following Secondary Immune Reaction in the Endolymphatic Sac.
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| Title: | Intercellular Adhesion Molecule-1 Expression in the Inner Ear of Rats following Secondary Immune Reaction in the Endolymphatic Sac. |
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| Authors: | Pawankar, Ruby, Tomiyama, Shunichi, Jinnouchi, Ken, Ikezono, Tetsuo, Nonaka, Manabu, Yagi, Toshiaki |
| Source: | Acta Oto-Laryngologica (Supplement). Nov1998 Supplement 539, Vol. 118, p5-14. 10p. |
| Subjects: | Cell adhesion molecules, Inner ear physiology, Rat physiology, Immune response, Secretion |
| Abstract: | An immunological aetiology for inner ear diseases has long been proposed. The endolymphatic sac (ES) is the only immunoprivileged site in the inner ear with a resident population of immunocompetent cells. By keyhole limpet hemocyanin (KLH) challenge into the ES of systemically pre-immunized guinea pigs, we previously demonstrated an infiltration of inflammatory cells into the perilymphatic space of the cochlea. In order to understand the mechanisms involved in the recruitment of immunocompetent cells into the inner ear, and their relation to the development of endolymphatic hydrops (EH), we investigated the expression and time-kinetics of intercellular adhesion molecule 1 (ICAM-1) in the inner ear of systemically pre-immunized rats after antigen (KLH) challenge into the ES, its relation to cell infiltration in the cochlea and subsequent development of EH. By immunohistochemistry, strong ICAM-1 expression was detected in the spiral ligament, suprastrial region, spiral prominence, spiral modiolar veins, spiral collecting venules, surface membrane of the perilymphatic compartment, perilymphatic space and ES of immunized rats, but not of control rats. ICAM-1 expression was detected at 5-6 h, peaked at 10-15 h, and gradually reduced by 2 weeks. Cell infiltration into the cochlea started at 6-12 h and peaked at day one. By 6 h, 50% of challenged rats developed EH. This figure rose to 70% at 12 h, and then gradually reduced. However, immunoreactivity for KLH (antigen) was only detected in the ES. These results emphasize that the sac is the central immunological organ of the inner ear, and suggest that ICAM-1 may play a pivotal role in the aetiology of immune-mediated inner ear diseases through the recruitment of immunocompetent cells into the inner ear and subsequent development of EH. [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Oto-Laryngologica (Supplement) is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 5323227 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Intercellular Adhesion Molecule-1 Expression in the Inner Ear of Rats following Secondary Immune Reaction in the Endolymphatic Sac. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Pawankar%2C+Ruby%22">Pawankar, Ruby</searchLink><br /><searchLink fieldCode="AR" term="%22Tomiyama%2C+Shunichi%22">Tomiyama, Shunichi</searchLink><br /><searchLink fieldCode="AR" term="%22Jinnouchi%2C+Ken%22">Jinnouchi, Ken</searchLink><br /><searchLink fieldCode="AR" term="%22Ikezono%2C+Tetsuo%22">Ikezono, Tetsuo</searchLink><br /><searchLink fieldCode="AR" term="%22Nonaka%2C+Manabu%22">Nonaka, Manabu</searchLink><br /><searchLink fieldCode="AR" term="%22Yagi%2C+Toshiaki%22">Yagi, Toshiaki</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Acta+Oto-Laryngologica+%28Supplement%29%22">Acta Oto-Laryngologica (Supplement)</searchLink>. Nov1998 Supplement 539, Vol. 118, p5-14. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Cell+adhesion+molecules%22">Cell adhesion molecules</searchLink><br /><searchLink fieldCode="DE" term="%22Inner+ear+physiology%22">Inner ear physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Rat+physiology%22">Rat physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Immune+response%22">Immune response</searchLink><br /><searchLink fieldCode="DE" term="%22Secretion%22">Secretion</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: An immunological aetiology for inner ear diseases has long been proposed. The endolymphatic sac (ES) is the only immunoprivileged site in the inner ear with a resident population of immunocompetent cells. By keyhole limpet hemocyanin (KLH) challenge into the ES of systemically pre-immunized guinea pigs, we previously demonstrated an infiltration of inflammatory cells into the perilymphatic space of the cochlea. In order to understand the mechanisms involved in the recruitment of immunocompetent cells into the inner ear, and their relation to the development of endolymphatic hydrops (EH), we investigated the expression and time-kinetics of intercellular adhesion molecule 1 (ICAM-1) in the inner ear of systemically pre-immunized rats after antigen (KLH) challenge into the ES, its relation to cell infiltration in the cochlea and subsequent development of EH. By immunohistochemistry, strong ICAM-1 expression was detected in the spiral ligament, suprastrial region, spiral prominence, spiral modiolar veins, spiral collecting venules, surface membrane of the perilymphatic compartment, perilymphatic space and ES of immunized rats, but not of control rats. ICAM-1 expression was detected at 5-6 h, peaked at 10-15 h, and gradually reduced by 2 weeks. Cell infiltration into the cochlea started at 6-12 h and peaked at day one. By 6 h, 50% of challenged rats developed EH. This figure rose to 70% at 12 h, and then gradually reduced. However, immunoreactivity for KLH (antigen) was only detected in the ES. These results emphasize that the sac is the central immunological organ of the inner ear, and suggest that ICAM-1 may play a pivotal role in the aetiology of immune-mediated inner ear diseases through the recruitment of immunocompetent cells into the inner ear and subsequent development of EH. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Acta Oto-Laryngologica (Supplement) is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1080/00016489850182053 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 5 Subjects: – SubjectFull: Cell adhesion molecules Type: general – SubjectFull: Inner ear physiology Type: general – SubjectFull: Rat physiology Type: general – SubjectFull: Immune response Type: general – SubjectFull: Secretion Type: general Titles: – TitleFull: Intercellular Adhesion Molecule-1 Expression in the Inner Ear of Rats following Secondary Immune Reaction in the Endolymphatic Sac. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Pawankar, Ruby – PersonEntity: Name: NameFull: Tomiyama, Shunichi – PersonEntity: Name: NameFull: Jinnouchi, Ken – PersonEntity: Name: NameFull: Ikezono, Tetsuo – PersonEntity: Name: NameFull: Nonaka, Manabu – PersonEntity: Name: NameFull: Yagi, Toshiaki IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 11 Text: Nov1998 Supplement 539 Type: published Y: 1998 Identifiers: – Type: issn-print Value: 03655237 Numbering: – Type: volume Value: 118 Titles: – TitleFull: Acta Oto-Laryngologica (Supplement) Type: main |
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