Nitric Oxide in Both Bronchoalveolar Lavage Fluid and Serum Is Associated With Pathogenesis and Severity of Antigen-Induced Pulmonary Inflammation in Rats.
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| Title: | Nitric Oxide in Both Bronchoalveolar Lavage Fluid and Serum Is Associated With Pathogenesis and Severity of Antigen-Induced Pulmonary Inflammation in Rats. |
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| Authors: | Yang, Xudong (AUTHOR), Sun, Qingzhu (AUTHOR), Raza Asim, M. B. (AUTHOR), Jiang, Xiaogang (AUTHOR), Zhong, Bo (AUTHOR), Shahzad, Muhammad (AUTHOR), Zhang, Fujun (AUTHOR), Han, Yan (AUTHOR), Lu, Shemin (AUTHOR) |
| Source: | Journal of Asthma. Mar2010, Vol. 47 Issue 2, p135-144. 10p. 2 Charts, 6 Graphs. |
| Subjects: | Bronchoalveolar lavage, Enzyme-linked immunosorbent assay, Nitric oxide, Obstructive lung diseases, Lung disease diagnosis |
| Abstract: | Background. Nitric oxide (NO) is considered as a hallmark for allergic airway inflammation in asthmatics and animal models. But the correlation between NO and antigen-induced pulmonary inflammation (AIPI), a rat model for asthma, in varying genetic background population has not been completely understood. Objective. The objective in this study is to observe the different responsiveness to AIPI in two commonly used inbred rat strains and verify the correlation between NO from different sources and pathological parameters of AIPI by using Dark Agouti (DA), E3, F1 (E3 × DA), and F2 rat populations. Methods. AIPI was induced by systemically immunizing and intranasally challenging E3, DA, F1 (DA × E3), and F2 rats with ovalbumin (OVA). Pathological changes and mucus secretion in lungs were observed after hematoxylin and eosin (HE) and periodic acid Schiff (PAS) staining, whereas eosinophils in bronchoalveolar lavage fluid (BALF) were counted after Giemsa staining. Delayed-type hyperresponsiveness was determined by subcutaneous injection of OVA in ear. Total immunoglobulin E (IgE) and OVA-specific IgG1 were detected with enzyme-linked immunosorbent assay (ELISA). NO concentration was measured by the Griess method. Results. DA rats were unresponsive to OVA treatment, whereas E3 rats were susceptible to AIPI. F1 rats manifested the same responsiveness to OVA treatment as DA rats, and individual F2 rats showed the variable severity of AIPI. NO concentration in BALF and serum was significantly elevated in E3 rats but not in DA and F1 rats after OVA treatment. In F2 rats, NO concentration in serum was positively correlated with eosinophils in BALF, total IgE, and pathological scores, whereas NO concentration in BALF correlated only with eosinophils in BALF and total IgE. Conclusion. DA and F1 rats are resistant, whereas E3 rats are sensitive, to AIPI. NO in serum can represent the severity of allergic inflammation and pathological changes in lungs in F2 population. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Asthma is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 53469465 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Nitric Oxide in Both Bronchoalveolar Lavage Fluid and Serum Is Associated With Pathogenesis and Severity of Antigen-Induced Pulmonary Inflammation in Rats. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Yang%2C+Xudong%22">Yang, Xudong</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sun%2C+Qingzhu%22">Sun, Qingzhu</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Raza+Asim%2C+M%2E+B%2E%22">Raza Asim, M. B.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jiang%2C+Xiaogang%22">Jiang, Xiaogang</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhong%2C+Bo%22">Zhong, Bo</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shahzad%2C+Muhammad%22">Shahzad, Muhammad</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Fujun%22">Zhang, Fujun</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Han%2C+Yan%22">Han, Yan</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lu%2C+Shemin%22">Lu, Shemin</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Asthma%22">Journal of Asthma</searchLink>. Mar2010, Vol. 47 Issue 2, p135-144. 10p. 2 Charts, 6 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Bronchoalveolar+lavage%22">Bronchoalveolar lavage</searchLink><br /><searchLink fieldCode="DE" term="%22Enzyme-linked+immunosorbent+assay%22">Enzyme-linked immunosorbent assay</searchLink><br /><searchLink fieldCode="DE" term="%22Nitric+oxide%22">Nitric oxide</searchLink><br /><searchLink fieldCode="DE" term="%22Obstructive+lung+diseases%22">Obstructive lung diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Lung+disease+diagnosis%22">Lung disease diagnosis</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Background. Nitric oxide (NO) is considered as a hallmark for allergic airway inflammation in asthmatics and animal models. But the correlation between NO and antigen-induced pulmonary inflammation (AIPI), a rat model for asthma, in varying genetic background population has not been completely understood. Objective. The objective in this study is to observe the different responsiveness to AIPI in two commonly used inbred rat strains and verify the correlation between NO from different sources and pathological parameters of AIPI by using Dark Agouti (DA), E3, F1 (E3 × DA), and F2 rat populations. Methods. AIPI was induced by systemically immunizing and intranasally challenging E3, DA, F1 (DA × E3), and F2 rats with ovalbumin (OVA). Pathological changes and mucus secretion in lungs were observed after hematoxylin and eosin (HE) and periodic acid Schiff (PAS) staining, whereas eosinophils in bronchoalveolar lavage fluid (BALF) were counted after Giemsa staining. Delayed-type hyperresponsiveness was determined by subcutaneous injection of OVA in ear. Total immunoglobulin E (IgE) and OVA-specific IgG1 were detected with enzyme-linked immunosorbent assay (ELISA). NO concentration was measured by the Griess method. Results. DA rats were unresponsive to OVA treatment, whereas E3 rats were susceptible to AIPI. F1 rats manifested the same responsiveness to OVA treatment as DA rats, and individual F2 rats showed the variable severity of AIPI. NO concentration in BALF and serum was significantly elevated in E3 rats but not in DA and F1 rats after OVA treatment. In F2 rats, NO concentration in serum was positively correlated with eosinophils in BALF, total IgE, and pathological scores, whereas NO concentration in BALF correlated only with eosinophils in BALF and total IgE. Conclusion. DA and F1 rats are resistant, whereas E3 rats are sensitive, to AIPI. NO in serum can represent the severity of allergic inflammation and pathological changes in lungs in F2 population. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Asthma is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3109/02770900903483808 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 135 Subjects: – SubjectFull: Bronchoalveolar lavage Type: general – SubjectFull: Enzyme-linked immunosorbent assay Type: general – SubjectFull: Nitric oxide Type: general – SubjectFull: Obstructive lung diseases Type: general – SubjectFull: Lung disease diagnosis Type: general Titles: – TitleFull: Nitric Oxide in Both Bronchoalveolar Lavage Fluid and Serum Is Associated With Pathogenesis and Severity of Antigen-Induced Pulmonary Inflammation in Rats. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Yang, Xudong – PersonEntity: Name: NameFull: Sun, Qingzhu – PersonEntity: Name: NameFull: Raza Asim, M. B. – PersonEntity: Name: NameFull: Jiang, Xiaogang – PersonEntity: Name: NameFull: Zhong, Bo – PersonEntity: Name: NameFull: Shahzad, Muhammad – PersonEntity: Name: NameFull: Zhang, Fujun – PersonEntity: Name: NameFull: Han, Yan – PersonEntity: Name: NameFull: Lu, Shemin IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2010 Type: published Y: 2010 Identifiers: – Type: issn-print Value: 02770903 Numbering: – Type: volume Value: 47 – Type: issue Value: 2 Titles: – TitleFull: Journal of Asthma Type: main |
| ResultId | 1 |
