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Early and delayed induction of immediate early gene expression in a novel focal cerebral ischemia model in the rat.

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Title: Early and delayed induction of immediate early gene expression in a novel focal cerebral ischemia model in the rat.
Authors: Johansson, Inga‐Maj (AUTHOR), Wester, Per (AUTHOR), Háková, Michaela (AUTHOR), Gu, WeiGang (AUTHOR), Seckl, Jonathan R. (AUTHOR), Olsson, Tommy (AUTHOR)
Source: European Journal of Neuroscience. Oct2000, Vol. 12 Issue 10, p3615-3625. 11p.
Subjects: Cerebral ischemia, Gene expression
Abstract: This study aimed at evaluating changes in expression of immediate early genes in a new photothrombotic focal ischemia model that exhibits late spontaneous reperfusion and morphological restoration in the region-at-risk within the cerebral cortex. Gene expression was studied with Northern blots, in situ hybridization and immunohistochemistry. At early time points (1-4 h), nerve growth factor-induced gene A and B, and c-fos mRNAs, were quickly induced throughout the ipsilateral cortex, with no obvious differences between the region-at-risk and remote cortical areas. High concentrations of nerve growth factor-induced gene A and c-Fos proteins were present within the region-at-risk even when cortical cerebral blood flow was as low as 40% of control values. At 4 h the nerve growth factor-induced gene A mRNA and protein expression was significantly decreased in the hippocampus vs. naive controls. However, a small decrease was also found in sham-operated and anaesthetized controls. A late induction, at 5 days, of c-fos and nerve growth factor-induced gene B mRNAs was seen bilaterally in the hippocampus and also, in the case of nerve growth factor induced-gene B, in the contralateral cortex. A complex pattern of changes in immediate early gene expression occurs after reversible focal cortical ischemia. This may be important for tissue recovery as well as neuropsychiatric symptoms after stroke. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Label: Title
  Group: Ti
  Data: Early and delayed induction of immediate early gene expression in a novel focal cerebral ischemia model in the rat.
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  Data: <searchLink fieldCode="AR" term="%22Johansson%2C+Inga‐Maj%22">Johansson, Inga‐Maj</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wester%2C+Per%22">Wester, Per</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Háková%2C+Michaela%22">Háková, Michaela</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gu%2C+WeiGang%22">Gu, WeiGang</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Seckl%2C+Jonathan+R%2E%22">Seckl, Jonathan R.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Olsson%2C+Tommy%22">Olsson, Tommy</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Oct2000, Vol. 12 Issue 10, p3615-3625. 11p.
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  Data: <searchLink fieldCode="DE" term="%22Cerebral+ischemia%22">Cerebral ischemia</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: This study aimed at evaluating changes in expression of immediate early genes in a new photothrombotic focal ischemia model that exhibits late spontaneous reperfusion and morphological restoration in the region-at-risk within the cerebral cortex. Gene expression was studied with Northern blots, in situ hybridization and immunohistochemistry. At early time points (1-4 h), nerve growth factor-induced gene A and B, and c-fos mRNAs, were quickly induced throughout the ipsilateral cortex, with no obvious differences between the region-at-risk and remote cortical areas. High concentrations of nerve growth factor-induced gene A and c-Fos proteins were present within the region-at-risk even when cortical cerebral blood flow was as low as 40% of control values. At 4 h the nerve growth factor-induced gene A mRNA and protein expression was significantly decreased in the hippocampus vs. naive controls. However, a small decrease was also found in sham-operated and anaesthetized controls. A late induction, at 5 days, of c-fos and nerve growth factor-induced gene B mRNAs was seen bilaterally in the hippocampus and also, in the case of nerve growth factor induced-gene B, in the contralateral cortex. A complex pattern of changes in immediate early gene expression occurs after reversible focal cortical ischemia. This may be important for tissue recovery as well as neuropsychiatric symptoms after stroke. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1046/j.1460-9568.2000.00252.x
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      – Code: eng
        Text: English
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      – SubjectFull: Gene expression
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              M: 10
              Text: Oct2000
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              Y: 2000
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