Cortical and nigral deafferentation and striatal cholinergic markers in the rat dorsal striatum: different effects on the expression of mRNAs encoding choline acetyltransferase and muscarinic m1 and m4 receptors.
Saved in:
| Title: | Cortical and nigral deafferentation and striatal cholinergic markers in the rat dorsal striatum: different effects on the expression of mRNAs encoding choline acetyltransferase and muscarinic m1 and m4 receptors. |
|---|---|
| Authors: | Kayadjanian, N. (AUTHOR), Schofield, W. N. (AUTHOR), Andren, J. (AUTHOR), Sirinathsinghji, D. J. S. (AUTHOR), Besson, M. ‐J. (AUTHOR) |
| Source: | European Journal of Neuroscience. Oct99, Vol. 11 Issue 10, p3659-3668. 10p. |
| Subjects: | Cholinergic receptors, Messenger RNA, Choline, Dopaminergic mechanisms |
| Abstract: | Abstract The regulation of the striatal m1 and m4 muscarinic receptor mRNA as well as the choline acetyltransferase (ChAT) mRNA expression by nigral dopaminergic and cortical glutamatergic afferent fibres was investigated using quantitative in situ hybridization histochemistry. The effects induced by a unilateral lesion of the medial forebrain bundle and a bilateral lesion of the sensorimotor (SM) cortex were analysed in the dorsal striatum 3 weeks after the lesions. Dopaminergic denervation of the striatum resulted in a marked decrease in the levels of m4 mRNA throughout the striatum, while the levels of muscarinic m1 mRNA and ChAT mRNA in cholinergic neurons were unaffected by the lesion. In contrast, following bilateral cortical ablation, the levels of the muscarinic m1 mRNA were significantly increased in the striatal projection area of the SM cortex, whereas the expression of m4 mRNA remained unchanged. Single cholinergic cell analysis by computer-assisted grain counting revealed a decreased labelling for ChAT mRNA per neuron following cortical ablation. However, in contrast to the topographical m1 mRNA changes, the decreased ChAT mRNA expression was evenly distributed within the striatum, suggesting an indirect cortical control upon striatal cholinergic interneurons. Altogether, these data suggest that dopaminergic nigral and glutamatergic cortical afferents modulate differentially cholinergic markers, at the pre- and post-synaptic levels. Beside the fact that nigral and cortical inputs exert an opposite control on cholinergic neurotransmission, our study further shows that this control involved different muscarinic receptor subtypes: the m4 and m1 receptors, respectively. [ABSTRACT FROM AUTHOR] |
| Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
|
Full text is not displayed to guests.
Login for full access.
|
|
| FullText | Links: – Type: pdflink Text: Availability: 1 |
|---|---|
| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 5979898 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Cortical and nigral deafferentation and striatal cholinergic markers in the rat dorsal striatum: different effects on the expression of mRNAs encoding choline acetyltransferase and muscarinic m1 and m4 receptors. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kayadjanian%2C+N%2E%22">Kayadjanian, N.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Schofield%2C+W%2E+N%2E%22">Schofield, W. N.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Andren%2C+J%2E%22">Andren, J.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sirinathsinghji%2C+D%2E+J%2E+S%2E%22">Sirinathsinghji, D. J. S.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Besson%2C+M%2E+‐J%2E%22">Besson, M. ‐J.</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Oct99, Vol. 11 Issue 10, p3659-3668. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Cholinergic+receptors%22">Cholinergic receptors</searchLink><br /><searchLink fieldCode="DE" term="%22Messenger+RNA%22">Messenger RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Choline%22">Choline</searchLink><br /><searchLink fieldCode="DE" term="%22Dopaminergic+mechanisms%22">Dopaminergic mechanisms</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract The regulation of the striatal m1 and m4 muscarinic receptor mRNA as well as the choline acetyltransferase (ChAT) mRNA expression by nigral dopaminergic and cortical glutamatergic afferent fibres was investigated using quantitative in situ hybridization histochemistry. The effects induced by a unilateral lesion of the medial forebrain bundle and a bilateral lesion of the sensorimotor (SM) cortex were analysed in the dorsal striatum 3 weeks after the lesions. Dopaminergic denervation of the striatum resulted in a marked decrease in the levels of m4 mRNA throughout the striatum, while the levels of muscarinic m1 mRNA and ChAT mRNA in cholinergic neurons were unaffected by the lesion. In contrast, following bilateral cortical ablation, the levels of the muscarinic m1 mRNA were significantly increased in the striatal projection area of the SM cortex, whereas the expression of m4 mRNA remained unchanged. Single cholinergic cell analysis by computer-assisted grain counting revealed a decreased labelling for ChAT mRNA per neuron following cortical ablation. However, in contrast to the topographical m1 mRNA changes, the decreased ChAT mRNA expression was evenly distributed within the striatum, suggesting an indirect cortical control upon striatal cholinergic interneurons. Altogether, these data suggest that dopaminergic nigral and glutamatergic cortical afferents modulate differentially cholinergic markers, at the pre- and post-synaptic levels. Beside the fact that nigral and cortical inputs exert an opposite control on cholinergic neurotransmission, our study further shows that this control involved different muscarinic receptor subtypes: the m4 and m1 receptors, respectively. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=5979898 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1046/j.1460-9568.1999.00788.x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 3659 Subjects: – SubjectFull: Cholinergic receptors Type: general – SubjectFull: Messenger RNA Type: general – SubjectFull: Choline Type: general – SubjectFull: Dopaminergic mechanisms Type: general Titles: – TitleFull: Cortical and nigral deafferentation and striatal cholinergic markers in the rat dorsal striatum: different effects on the expression of mRNAs encoding choline acetyltransferase and muscarinic m1 and m4 receptors. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kayadjanian, N. – PersonEntity: Name: NameFull: Schofield, W. N. – PersonEntity: Name: NameFull: Andren, J. – PersonEntity: Name: NameFull: Sirinathsinghji, D. J. S. – PersonEntity: Name: NameFull: Besson, M. ‐J. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: Oct99 Type: published Y: 1999 Identifiers: – Type: issn-print Value: 0953816X Numbering: – Type: volume Value: 11 – Type: issue Value: 10 Titles: – TitleFull: European Journal of Neuroscience Type: main |
| ResultId | 1 |
