View in EDS HTML Full Text PDF Full Text

Growth responses of different subpopulations of adult sensory neurons to neurotrophic factors in vitro.

Saved in:
Bibliographic Details
Title: Growth responses of different subpopulations of adult sensory neurons to neurotrophic factors in vitro.
Authors: Gavazzi, Isabella (AUTHOR), Kumar, Robin D. C. (AUTHOR), McMahon, Stephen B. (AUTHOR), Cohen, James (AUTHOR)
Source: European Journal of Neuroscience. Oct99, Vol. 11 Issue 10, p3405-3414. 10p.
Subjects: Sensory neurons, Neurotrophins
Abstract: Abstract Different subpopulations of adult primary sensory neurons in the dorsal root ganglia express receptors for different trophic factors, and are therefore potentially responsive to distinct trophic signals. We have compared the effect of the neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and NT-3, and of glial cell line-derived neurotrophic factor (GDNF) on neurite outgrowth in dissociated cultures of sensory neurons from the lumbar ganglia of young adult rats, and attempted to establish subset-specific effects of these trophic factors. We analysed three parameters of neurite growth (percentage of process-bearing neurons, length of longest neurite and total neurite length), which may correlate with particular types of axon growth in vivo, and may therefore respond differently to trophic factor presence. Our results showed that percentage of process-bearing neurons and total neurite length were influenced by trophic factors, whilst the length of the longest neurite was trophic factor independent. Only NGF and GDNF were found to enhance significantly the proportion of process-bearing neurons in vitro. GDNF was more effective than NGF on small, IB4– neurons, which are known to develop GDNF responsiveness early in postnatal development. NGF, and to a much lesser extent GDNF, enhanced the total length of the neurites produced by neurons in culture. BDNF exerted an inhibitory effect on growth, and both BDNF and NT-3 could partially block some of the growth-promoting effects of NGF on specific neuronal subpopulations. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: pbh
DbLabel: Psychology and Behavioral Sciences Collection
An: 5979926
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Growth responses of different subpopulations of adult sensory neurons to neurotrophic factors in vitro.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Gavazzi%2C+Isabella%22">Gavazzi, Isabella</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kumar%2C+Robin+D%2E+C%2E%22">Kumar, Robin D. C.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22McMahon%2C+Stephen+B%2E%22">McMahon, Stephen B.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cohen%2C+James%22">Cohen, James</searchLink> (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Oct99, Vol. 11 Issue 10, p3405-3414. 10p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Sensory+neurons%22">Sensory neurons</searchLink><br /><searchLink fieldCode="DE" term="%22Neurotrophins%22">Neurotrophins</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract Different subpopulations of adult primary sensory neurons in the dorsal root ganglia express receptors for different trophic factors, and are therefore potentially responsive to distinct trophic signals. We have compared the effect of the neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and NT-3, and of glial cell line-derived neurotrophic factor (GDNF) on neurite outgrowth in dissociated cultures of sensory neurons from the lumbar ganglia of young adult rats, and attempted to establish subset-specific effects of these trophic factors. We analysed three parameters of neurite growth (percentage of process-bearing neurons, length of longest neurite and total neurite length), which may correlate with particular types of axon growth in vivo, and may therefore respond differently to trophic factor presence. Our results showed that percentage of process-bearing neurons and total neurite length were influenced by trophic factors, whilst the length of the longest neurite was trophic factor independent. Only NGF and GDNF were found to enhance significantly the proportion of process-bearing neurons in vitro. GDNF was more effective than NGF on small, IB4– neurons, which are known to develop GDNF responsiveness early in postnatal development. NGF, and to a much lesser extent GDNF, enhanced the total length of the neurites produced by neurons in culture. BDNF exerted an inhibitory effect on growth, and both BDNF and NT-3 could partially block some of the growth-promoting effects of NGF on specific neuronal subpopulations. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=5979926
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1046/j.1460-9568.1999.00756.x
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 3405
    Subjects:
      – SubjectFull: Sensory neurons
        Type: general
      – SubjectFull: Neurotrophins
        Type: general
    Titles:
      – TitleFull: Growth responses of different subpopulations of adult sensory neurons to neurotrophic factors in vitro.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Gavazzi, Isabella
      – PersonEntity:
          Name:
            NameFull: Kumar, Robin D. C.
      – PersonEntity:
          Name:
            NameFull: McMahon, Stephen B.
      – PersonEntity:
          Name:
            NameFull: Cohen, James
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 10
              Text: Oct99
              Type: published
              Y: 1999
          Identifiers:
            – Type: issn-print
              Value: 0953816X
          Numbering:
            – Type: volume
              Value: 11
            – Type: issue
              Value: 10
          Titles:
            – TitleFull: European Journal of Neuroscience
              Type: main
ResultId 1