Periwound dopaminergic sprouting is dependent on numbers of wound macrophages.
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| Title: | Periwound dopaminergic sprouting is dependent on numbers of wound macrophages. |
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| Authors: | Batchelor, P. E. (AUTHOR), Porritt, M. J. (AUTHOR), Nilsson, S. K. (AUTHOR), Bertoncello, I. (AUTHOR), Donnan, G. A. (AUTHOR), Howells, D. W. (AUTHOR) |
| Source: | European Journal of Neuroscience. Mar2002, Vol. 15 Issue 5, p826-832. 7p. |
| Subjects: | Macrophages, Dopaminergic neurons, Rat physiology |
| Abstract: | Abstract Injury to many regions of the central nervous system, including the striatum, results in a periwound or ‘abortive’ sprouting response. In order to directly evaluate whether macrophages play an important role in stimulating periwound sprouting, osteopetrotic (op/op) mice, which when young are deficient in a variety of macrophage subtypes, were given striatal wounds and the degree of dopaminergic sprouting subsequently assessed. Two weeks postinjury, significantly fewer wound macrophages were present in the striata of op/op mice compared with controls (144 ± 30.1 in op/op mice vs. 416.6 ± 82.3 in controls, P < 0.005, analysis performed on a section transecting the middle of the wound). Dopamine transporter immunohistochemistry revealed a marked decrease in the intensity of periwound sprouting in the op/op group of animals. Quantification of this effect using [H3 ]-mazindol autoradiography confirmed that periwound sprouting was reduced significantly in the op/op mice compared with controls (71.4 ± 21.7 fmol/mg protein in op/op mice vs. 210.7 ± 27.1 fmol/mg protein in controls, P < 0.0005). In the two groups of animals the magnitude of the sprouting response in individuals was closely correlated with the number of wound macrophages (R = 0.83, R 2 = 0.69). Our findings provide strong support for the crucial involvement of macrophages in inducing dopaminergic sprouting after striatal injury. [ABSTRACT FROM AUTHOR] |
| Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 6371018 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Periwound dopaminergic sprouting is dependent on numbers of wound macrophages. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Batchelor%2C+P%2E+E%2E%22">Batchelor, P. E.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Porritt%2C+M%2E+J%2E%22">Porritt, M. J.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nilsson%2C+S%2E+K%2E%22">Nilsson, S. K.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bertoncello%2C+I%2E%22">Bertoncello, I.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Donnan%2C+G%2E+A%2E%22">Donnan, G. A.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Howells%2C+D%2E+W%2E%22">Howells, D. W.</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Mar2002, Vol. 15 Issue 5, p826-832. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Macrophages%22">Macrophages</searchLink><br /><searchLink fieldCode="DE" term="%22Dopaminergic+neurons%22">Dopaminergic neurons</searchLink><br /><searchLink fieldCode="DE" term="%22Rat+physiology%22">Rat physiology</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract Injury to many regions of the central nervous system, including the striatum, results in a periwound or ‘abortive’ sprouting response. In order to directly evaluate whether macrophages play an important role in stimulating periwound sprouting, osteopetrotic (op/op) mice, which when young are deficient in a variety of macrophage subtypes, were given striatal wounds and the degree of dopaminergic sprouting subsequently assessed. Two weeks postinjury, significantly fewer wound macrophages were present in the striata of op/op mice compared with controls (144 ± 30.1 in op/op mice vs. 416.6 ± 82.3 in controls, P < 0.005, analysis performed on a section transecting the middle of the wound). Dopamine transporter immunohistochemistry revealed a marked decrease in the intensity of periwound sprouting in the op/op group of animals. Quantification of this effect using [H3 ]-mazindol autoradiography confirmed that periwound sprouting was reduced significantly in the op/op mice compared with controls (71.4 ± 21.7 fmol/mg protein in op/op mice vs. 210.7 ± 27.1 fmol/mg protein in controls, P < 0.0005). In the two groups of animals the magnitude of the sprouting response in individuals was closely correlated with the number of wound macrophages (R = 0.83, R 2 = 0.69). Our findings provide strong support for the crucial involvement of macrophages in inducing dopaminergic sprouting after striatal injury. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1046/j.1460-9568.2002.01914.x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 826 Subjects: – SubjectFull: Macrophages Type: general – SubjectFull: Dopaminergic neurons Type: general – SubjectFull: Rat physiology Type: general Titles: – TitleFull: Periwound dopaminergic sprouting is dependent on numbers of wound macrophages. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Batchelor, P. E. – PersonEntity: Name: NameFull: Porritt, M. J. – PersonEntity: Name: NameFull: Nilsson, S. K. – PersonEntity: Name: NameFull: Bertoncello, I. – PersonEntity: Name: NameFull: Donnan, G. A. – PersonEntity: Name: NameFull: Howells, D. W. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2002 Type: published Y: 2002 Identifiers: – Type: issn-print Value: 0953816X Numbering: – Type: volume Value: 15 – Type: issue Value: 5 Titles: – TitleFull: European Journal of Neuroscience Type: main |
| ResultId | 1 |
