A Burkholderia pseudomallei Toxin Inhibits Helicase Activity of Translation Factor elF4A.

Saved in:

Bibliographic Details
Title:	A Burkholderia pseudomallei Toxin Inhibits Helicase Activity of Translation Factor elF4A.
Authors:	Cruz-Migoni, Abimael, Hautbergue, Guillaume M., Artymiuk, Peter J., Baker, Patrick J., Bokori-Brown, Monika, Chung-Te Chang, Dickman, Mark J., Essex-Lopresti, Angela, Harding, Sarah V., Mahadi, Nor Muhammad, Marshall, Laura E., Mobbs, George W., Mohamed, Rahmah, Nathan, Sheila, Ngugi, Sarah A., Ong, Catherine, Wen Fong Ooi, Partridge, Lynda J., Phillips, Helen L., Raih, M. Firdaus
Source:	Science (pre-March 2025). 11/11/2011, Vol. 334 Issue 6057, p821-824. 4p.
Subjects:	Molecular biology, Burkholderia infections, Melioidosis, DNA helicases, Eukaryotic cells, Deamination, Glutamine, Genetic translation
Abstract:	The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor elF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1. [ABSTRACT FROM AUTHOR]
	Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database:	Psychology and Behavioral Sciences Collection

Description
Abstract:	The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor elF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1. [ABSTRACT FROM AUTHOR]
ISSN:	00368075
DOI:	10.1126/science.1211915