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Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq.

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Title: Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq.
Authors: Dominissini, Dan, Moshitch-Moshkovitz, Sharon, Schwartz, Schraga, Salmon-Divon, Mali, Ungar, Lior, Osenberg, Sivan, Cesarkas, Karen, Jacob-Hirsch, Jasmine, Amariglio, Ninette, Kupiec, Martin, Sorek, Rotem, Rechavi, Gideon
Source: Nature. 5/10/2012, Vol. 485 Issue 7397, p201-206. 6p.
Subjects: RNA, Genetic regulation, Gene expression, Messenger RNA, Apoptosis, Nucleic acids
Abstract: An extensive repertoire of modifications is known to underlie the versatile coding, structural and catalytic functions of RNA, but it remains largely uncharted territory. Although biochemical studies indicate that N6-methyladenosine (m6A) is the most prevalent internal modification in messenger RNA, an in-depth study of its distribution and functions has been impeded by a lack of robust analytical methods. Here we present the human and mouse m6A modification landscape in a transcriptome-wide manner, using a novel approach, m6A-seq, based on antibody-mediated capture and massively parallel sequencing. We identify over 12,000 m6A sites characterized by a typical consensus in the transcripts of more than 7,000 human genes. Sites preferentially appear in two distinct landmarks-around stop codons and within long internal exons-and are highly conserved between human and mouse. Although most sites are well preserved across normal and cancerous tissues and in response to various stimuli, a subset of stimulus-dependent, dynamically modulated sites is identified. Silencing the m6A methyltransferase significantly affects gene expression and alternative splicing patterns, resulting in modulation of the p53 (also known as TP53) signalling pathway and apoptosis. Our findings therefore suggest that RNA decoration by m6A has a fundamental role in regulation of gene expression. [ABSTRACT FROM AUTHOR]
Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Topology of the human and mouse m<superscript>6</superscript>A RNA methylomes revealed by m<superscript>6</superscript>A-seq.
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  Data: <searchLink fieldCode="AR" term="%22Dominissini%2C+Dan%22">Dominissini, Dan</searchLink><br /><searchLink fieldCode="AR" term="%22Moshitch-Moshkovitz%2C+Sharon%22">Moshitch-Moshkovitz, Sharon</searchLink><br /><searchLink fieldCode="AR" term="%22Schwartz%2C+Schraga%22">Schwartz, Schraga</searchLink><br /><searchLink fieldCode="AR" term="%22Salmon-Divon%2C+Mali%22">Salmon-Divon, Mali</searchLink><br /><searchLink fieldCode="AR" term="%22Ungar%2C+Lior%22">Ungar, Lior</searchLink><br /><searchLink fieldCode="AR" term="%22Osenberg%2C+Sivan%22">Osenberg, Sivan</searchLink><br /><searchLink fieldCode="AR" term="%22Cesarkas%2C+Karen%22">Cesarkas, Karen</searchLink><br /><searchLink fieldCode="AR" term="%22Jacob-Hirsch%2C+Jasmine%22">Jacob-Hirsch, Jasmine</searchLink><br /><searchLink fieldCode="AR" term="%22Amariglio%2C+Ninette%22">Amariglio, Ninette</searchLink><br /><searchLink fieldCode="AR" term="%22Kupiec%2C+Martin%22">Kupiec, Martin</searchLink><br /><searchLink fieldCode="AR" term="%22Sorek%2C+Rotem%22">Sorek, Rotem</searchLink><br /><searchLink fieldCode="AR" term="%22Rechavi%2C+Gideon%22">Rechavi, Gideon</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Nature%22">Nature</searchLink>. 5/10/2012, Vol. 485 Issue 7397, p201-206. 6p.
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  Data: An extensive repertoire of modifications is known to underlie the versatile coding, structural and catalytic functions of RNA, but it remains largely uncharted territory. Although biochemical studies indicate that N6-methyladenosine (m6A) is the most prevalent internal modification in messenger RNA, an in-depth study of its distribution and functions has been impeded by a lack of robust analytical methods. Here we present the human and mouse m6A modification landscape in a transcriptome-wide manner, using a novel approach, m6A-seq, based on antibody-mediated capture and massively parallel sequencing. We identify over 12,000 m6A sites characterized by a typical consensus in the transcripts of more than 7,000 human genes. Sites preferentially appear in two distinct landmarks-around stop codons and within long internal exons-and are highly conserved between human and mouse. Although most sites are well preserved across normal and cancerous tissues and in response to various stimuli, a subset of stimulus-dependent, dynamically modulated sites is identified. Silencing the m6A methyltransferase significantly affects gene expression and alternative splicing patterns, resulting in modulation of the p53 (also known as TP53) signalling pathway and apoptosis. Our findings therefore suggest that RNA decoration by m6A has a fundamental role in regulation of gene expression. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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