Prevalence of Human Papillomavirus in Squamous Cell Carcinoma of the Head and Neck Determined by Polymerase Chain Reaction and Southern Blot Hybridization: Proposal for Optimized Diagnostic Requirements.
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| Title: | Prevalence of Human Papillomavirus in Squamous Cell Carcinoma of the Head and Neck Determined by Polymerase Chain Reaction and Southern Blot Hybridization: Proposal for Optimized Diagnostic Requirements. |
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| Authors: | HOFFMANN, MARKUS, KAHN, TOMAS, MAHNKE, CHRISTOPH G., GOEROEGH, TIBOR, LIPPERT, BURKARD M., WERNER, JOCHEN A. |
| Source: | Acta Oto-Laryngologica. Jan98, Vol. 118 Issue 1, p138-144. 7p. |
| Subjects: | Squamous cell carcinoma, Head & neck cancer, Papillomaviruses, Polymerase chain reaction |
| Abstract: | Human papillomaviruses (HPV) are discussed as cofactors in the carcinogenesis of squamous cell carcinoma of the head and neck (SCCHN). The prevalence of HPV infection in SCCHN is the subject of controversy since reported HPV prevalences range from less than 10% to almost 100%, depending mainly on the detection method employed. This study presents a realistic approximation to the real prevalence of HPV in SSCHN by applying polymerase chain reaction (PCR) and Southern blot hybridization (SBH), which are the most sensitive and specific HPV detection methods. Diagnostic procedures were optimized by applying a ''hot-start'' PCR protocol followed by a confirmatory SBH of the PCR products to reactions using both type-specific and consensus primers and probes. DNA of 75 tumour samples and 22 normal mucosa samples of the same patients were investigated. In 14 cases genomic SBH using complete HPV genomes as probes was performed additionally. HPV DNA was detected in 17/75 (22.7%) SCCHN specimens. HPV 16 was identified in four cases, HPV 45 in three cases, and HPV 6 and 18 in one case each. Hot-start PCR and SBH are the most reliable HPV detection methods, as they minimize both false-positive and false-negative results. With these methods, a HPV prevalence of 23% was achieved, which can be assumed to be representative for comparable study populations. The significant number of positives detected only by consensus primer PCR, along with the identification of HPV 45, indicate that further HPV types may play an important role in the genesis of SCCHN. [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 7613855 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Prevalence of Human Papillomavirus in Squamous Cell Carcinoma of the Head and Neck Determined by Polymerase Chain Reaction and Southern Blot Hybridization: Proposal for Optimized Diagnostic Requirements. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22HOFFMANN%2C+MARKUS%22">HOFFMANN, MARKUS</searchLink><br /><searchLink fieldCode="AR" term="%22KAHN%2C+TOMAS%22">KAHN, TOMAS</searchLink><br /><searchLink fieldCode="AR" term="%22MAHNKE%2C+CHRISTOPH+G%2E%22">MAHNKE, CHRISTOPH G.</searchLink><br /><searchLink fieldCode="AR" term="%22GOEROEGH%2C+TIBOR%22">GOEROEGH, TIBOR</searchLink><br /><searchLink fieldCode="AR" term="%22LIPPERT%2C+BURKARD+M%2E%22">LIPPERT, BURKARD M.</searchLink><br /><searchLink fieldCode="AR" term="%22WERNER%2C+JOCHEN+A%2E%22">WERNER, JOCHEN A.</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Acta+Oto-Laryngologica%22">Acta Oto-Laryngologica</searchLink>. Jan98, Vol. 118 Issue 1, p138-144. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Squamous+cell+carcinoma%22">Squamous cell carcinoma</searchLink><br /><searchLink fieldCode="DE" term="%22Head+%26+neck+cancer%22">Head & neck cancer</searchLink><br /><searchLink fieldCode="DE" term="%22Papillomaviruses%22">Papillomaviruses</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Human papillomaviruses (HPV) are discussed as cofactors in the carcinogenesis of squamous cell carcinoma of the head and neck (SCCHN). The prevalence of HPV infection in SCCHN is the subject of controversy since reported HPV prevalences range from less than 10% to almost 100%, depending mainly on the detection method employed. This study presents a realistic approximation to the real prevalence of HPV in SSCHN by applying polymerase chain reaction (PCR) and Southern blot hybridization (SBH), which are the most sensitive and specific HPV detection methods. Diagnostic procedures were optimized by applying a ''hot-start'' PCR protocol followed by a confirmatory SBH of the PCR products to reactions using both type-specific and consensus primers and probes. DNA of 75 tumour samples and 22 normal mucosa samples of the same patients were investigated. In 14 cases genomic SBH using complete HPV genomes as probes was performed additionally. HPV DNA was detected in 17/75 (22.7%) SCCHN specimens. HPV 16 was identified in four cases, HPV 45 in three cases, and HPV 6 and 18 in one case each. Hot-start PCR and SBH are the most reliable HPV detection methods, as they minimize both false-positive and false-negative results. With these methods, a HPV prevalence of 23% was achieved, which can be assumed to be representative for comparable study populations. The significant number of positives detected only by consensus primer PCR, along with the identification of HPV 45, indicate that further HPV types may play an important role in the genesis of SCCHN. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1080/00016489850155279 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 138 Subjects: – SubjectFull: Squamous cell carcinoma Type: general – SubjectFull: Head & neck cancer Type: general – SubjectFull: Papillomaviruses Type: general – SubjectFull: Polymerase chain reaction Type: general Titles: – TitleFull: Prevalence of Human Papillomavirus in Squamous Cell Carcinoma of the Head and Neck Determined by Polymerase Chain Reaction and Southern Blot Hybridization: Proposal for Optimized Diagnostic Requirements. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: HOFFMANN, MARKUS – PersonEntity: Name: NameFull: KAHN, TOMAS – PersonEntity: Name: NameFull: MAHNKE, CHRISTOPH G. – PersonEntity: Name: NameFull: GOEROEGH, TIBOR – PersonEntity: Name: NameFull: LIPPERT, BURKARD M. – PersonEntity: Name: NameFull: WERNER, JOCHEN A. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan98 Type: published Y: 1998 Identifiers: – Type: issn-print Value: 00016489 Numbering: – Type: volume Value: 118 – Type: issue Value: 1 Titles: – TitleFull: Acta Oto-Laryngologica Type: main |
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