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Immune self-reactivity triggered by drug-modified HLA-peptide repertoire.

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Title: Immune self-reactivity triggered by drug-modified HLA-peptide repertoire.
Authors: Illing, Patricia T., Vivian, Julian P., Dudek, Nadine L., Kostenko, Lyudmila, Chen, Zhenjun, Bharadwaj, Mandvi, Miles, John J., Kjer-Nielsen, Lars, Gras, Stephanie, Williamson, Nicholas A., Burrows, Scott R., Purcell, Anthony W., Rossjohn, Jamie, McCluskey, James
Source: Nature. 6/28/2012, Vol. 486 Issue 7404, p554-558. 5p. 1 Diagram, 3 Graphs.
Subjects: Reactivity (Chemistry), HLA histocompatibility antigens, Peptides, T cells, Abacavir
Abstract: Human leukocyte antigens (HLAs) are highly polymorphic proteins that initiate immunity by presenting pathogen-derived peptides to T?cells. HLA polymorphisms mostly map to the antigen-binding cleft, thereby diversifying the repertoire of self-derived and pathogen-derived peptide antigens selected by different HLA allotypes. A growing number of immunologically based drug reactions, including abacavir hypersensitivity syndrome (AHS) and carbamazepine-induced Stevens-Johnson syndrome (SJS), are associated with specific HLA alleles. However, little is known about the underlying mechanisms of these associations, including AHS, a prototypical HLA-associated drug reaction occurring exclusively in individuals with the common histocompatibility allele HLA-B*57:01, and with a relative risk of more than 1,000 (refs?6, 7). We show that unmodified abacavir binds non-covalently to HLA-B*57:01, lying across the bottom of the antigen-binding cleft and reaching into the F-pocket, where a carboxy-terminal tryptophan typically anchors peptides bound to HLA-B*57:01. Abacavir binds with exquisite specificity to HLA-B*57:01, changing the shape and chemistry of the antigen-binding cleft, thereby altering the repertoire of endogenous peptides that can bind HLA-B*57:01. In this way, abacavir guides the selection of new endogenous peptides, inducing a marked alteration in 'immunological self'. The resultant peptide-centric 'altered self' activates abacavir-specific T-cells, thereby driving polyclonal CD8 T-cell activation and a systemic reaction manifesting as AHS. We also show that carbamazepine, a widely used anti-epileptic drug associated with hypersensitivity reactions in HLA-B*15:02 individuals, binds to this allotype, producing alterations in the repertoire of presented self peptides. Our findings simultaneously highlight the importance of HLA polymorphism in the evolution of pharmacogenomics and provide a general mechanism for some of the growing number of HLA-linked hypersensitivities that involve small-molecule drugs. [ABSTRACT FROM AUTHOR]
Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Immune self-reactivity triggered by drug-modified HLA-peptide repertoire.
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  Data: <searchLink fieldCode="AR" term="%22Illing%2C+Patricia+T%2E%22">Illing, Patricia T.</searchLink><br /><searchLink fieldCode="AR" term="%22Vivian%2C+Julian+P%2E%22">Vivian, Julian P.</searchLink><br /><searchLink fieldCode="AR" term="%22Dudek%2C+Nadine+L%2E%22">Dudek, Nadine L.</searchLink><br /><searchLink fieldCode="AR" term="%22Kostenko%2C+Lyudmila%22">Kostenko, Lyudmila</searchLink><br /><searchLink fieldCode="AR" term="%22Chen%2C+Zhenjun%22">Chen, Zhenjun</searchLink><br /><searchLink fieldCode="AR" term="%22Bharadwaj%2C+Mandvi%22">Bharadwaj, Mandvi</searchLink><br /><searchLink fieldCode="AR" term="%22Miles%2C+John+J%2E%22">Miles, John J.</searchLink><br /><searchLink fieldCode="AR" term="%22Kjer-Nielsen%2C+Lars%22">Kjer-Nielsen, Lars</searchLink><br /><searchLink fieldCode="AR" term="%22Gras%2C+Stephanie%22">Gras, Stephanie</searchLink><br /><searchLink fieldCode="AR" term="%22Williamson%2C+Nicholas+A%2E%22">Williamson, Nicholas A.</searchLink><br /><searchLink fieldCode="AR" term="%22Burrows%2C+Scott+R%2E%22">Burrows, Scott R.</searchLink><br /><searchLink fieldCode="AR" term="%22Purcell%2C+Anthony+W%2E%22">Purcell, Anthony W.</searchLink><br /><searchLink fieldCode="AR" term="%22Rossjohn%2C+Jamie%22">Rossjohn, Jamie</searchLink><br /><searchLink fieldCode="AR" term="%22McCluskey%2C+James%22">McCluskey, James</searchLink>
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  Data: Human leukocyte antigens (HLAs) are highly polymorphic proteins that initiate immunity by presenting pathogen-derived peptides to T?cells. HLA polymorphisms mostly map to the antigen-binding cleft, thereby diversifying the repertoire of self-derived and pathogen-derived peptide antigens selected by different HLA allotypes. A growing number of immunologically based drug reactions, including abacavir hypersensitivity syndrome (AHS) and carbamazepine-induced Stevens-Johnson syndrome (SJS), are associated with specific HLA alleles. However, little is known about the underlying mechanisms of these associations, including AHS, a prototypical HLA-associated drug reaction occurring exclusively in individuals with the common histocompatibility allele HLA-B*57:01, and with a relative risk of more than 1,000 (refs?6, 7). We show that unmodified abacavir binds non-covalently to HLA-B*57:01, lying across the bottom of the antigen-binding cleft and reaching into the F-pocket, where a carboxy-terminal tryptophan typically anchors peptides bound to HLA-B*57:01. Abacavir binds with exquisite specificity to HLA-B*57:01, changing the shape and chemistry of the antigen-binding cleft, thereby altering the repertoire of endogenous peptides that can bind HLA-B*57:01. In this way, abacavir guides the selection of new endogenous peptides, inducing a marked alteration in 'immunological self'. The resultant peptide-centric 'altered self' activates abacavir-specific T-cells, thereby driving polyclonal CD8 T-cell activation and a systemic reaction manifesting as AHS. We also show that carbamazepine, a widely used anti-epileptic drug associated with hypersensitivity reactions in HLA-B*15:02 individuals, binds to this allotype, producing alterations in the repertoire of presented self peptides. Our findings simultaneously highlight the importance of HLA polymorphism in the evolution of pharmacogenomics and provide a general mechanism for some of the growing number of HLA-linked hypersensitivities that involve small-molecule drugs. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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