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Jagged1-mediated Notch signaling regulates mammalian inner ear development independent of lateral inhibition.

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Title: Jagged1-mediated Notch signaling regulates mammalian inner ear development independent of lateral inhibition.
Authors: Hao, Jin (AUTHOR), Koesters, Robert (AUTHOR), Bouchard, Maxime (AUTHOR), Gridley, Thomas (AUTHOR), Pfannenstiel, Susanna (AUTHOR), Plinkert, Peter K. (AUTHOR), Zhang, Luo (AUTHOR), Praetorius, Mark (AUTHOR)
Source: Acta Oto-Laryngologica. Oct2012, Vol. 132 Issue 10, p1028-1035. 8p.
Subjects: Inner ear, Hair cells, Sensitivity analysis, Gene targeting, Brain stem, Gene expression, Animal experimentation, Genetics, Mammals, Mice, Proteins, Research funding, Data analysis software, Mann Whitney U Test
Abstract: Conclusion: Jagged1-mediated Notch signaling regulates hair cell (HC) production in a distinct way rather than lateral inhibition mediated by Hes1 and Hes5. Jagged1 may interact with Notch3, probably via candidate downstream mediators Hesr1 and Hesr2, regulating the prosensory formation in the early stage. Objectives: To explore the function of the Jagged1-mediated Notch signaling pathway in mammalian inner ear development and its possible mechanism. Methods: Using conditional gene targeting, a novel Jagged1 conditional knockout (Jag1-cko), Pax8cre/+; Jag1flox/flox, was established. The auditory brainstem response and swim ability test were utilized to identify functional disability. The expression of Jagged1, Notch3, Hes1, Hesr1, and Hesr2 was detected by immunofluorescence and immunohistochemistry. Results: Our Jag1-cko model was established and survived well. It presented hearing impairment and balance disturbance with 'waltzing' behavior. Cochleae and vestibular apparatus were all found in our Jag1-cko model. Patch deficiency of outer hair cells (OHCs) was found on the apical and middle turns of the auditory epithelium. OHCs were totally missing on the basal turn. The stereociliary bundles were disorientated on the cristae. Unlike Hes1, no expression of Notch3, Hesr1, and Hesr2 was found on embryonic day 13.5 of the Jag1-cko model. [ABSTRACT FROM AUTHOR]
Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Label: Title
  Group: Ti
  Data: Jagged1-mediated Notch signaling regulates mammalian inner ear development independent of lateral inhibition.
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  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Hao%2C+Jin%22">Hao, Jin</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Koesters%2C+Robert%22">Koesters, Robert</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bouchard%2C+Maxime%22">Bouchard, Maxime</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gridley%2C+Thomas%22">Gridley, Thomas</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pfannenstiel%2C+Susanna%22">Pfannenstiel, Susanna</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Plinkert%2C+Peter+K%2E%22">Plinkert, Peter K.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Luo%22">Zhang, Luo</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Praetorius%2C+Mark%22">Praetorius, Mark</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Acta+Oto-Laryngologica%22">Acta Oto-Laryngologica</searchLink>. Oct2012, Vol. 132 Issue 10, p1028-1035. 8p.
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  Data: <searchLink fieldCode="DE" term="%22Inner+ear%22">Inner ear</searchLink><br /><searchLink fieldCode="DE" term="%22Hair+cells%22">Hair cells</searchLink><br /><searchLink fieldCode="DE" term="%22Sensitivity+analysis%22">Sensitivity analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+targeting%22">Gene targeting</searchLink><br /><searchLink fieldCode="DE" term="%22Brain+stem%22">Brain stem</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+experimentation%22">Animal experimentation</searchLink><br /><searchLink fieldCode="DE" term="%22Genetics%22">Genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Mammals%22">Mammals</searchLink><br /><searchLink fieldCode="DE" term="%22Mice%22">Mice</searchLink><br /><searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Research+funding%22">Research funding</searchLink><br /><searchLink fieldCode="DE" term="%22Data+analysis+software%22">Data analysis software</searchLink><br /><searchLink fieldCode="DE" term="%22Mann+Whitney+U+Test%22">Mann Whitney U Test</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Conclusion: Jagged1-mediated Notch signaling regulates hair cell (HC) production in a distinct way rather than lateral inhibition mediated by Hes1 and Hes5. Jagged1 may interact with Notch3, probably via candidate downstream mediators Hesr1 and Hesr2, regulating the prosensory formation in the early stage. Objectives: To explore the function of the Jagged1-mediated Notch signaling pathway in mammalian inner ear development and its possible mechanism. Methods: Using conditional gene targeting, a novel Jagged1 conditional knockout (Jag1-cko), Pax8cre/+; Jag1flox/flox, was established. The auditory brainstem response and swim ability test were utilized to identify functional disability. The expression of Jagged1, Notch3, Hes1, Hesr1, and Hesr2 was detected by immunofluorescence and immunohistochemistry. Results: Our Jag1-cko model was established and survived well. It presented hearing impairment and balance disturbance with 'waltzing' behavior. Cochleae and vestibular apparatus were all found in our Jag1-cko model. Patch deficiency of outer hair cells (OHCs) was found on the apical and middle turns of the auditory epithelium. OHCs were totally missing on the basal turn. The stereociliary bundles were disorientated on the cristae. Unlike Hes1, no expression of Notch3, Hesr1, and Hesr2 was found on embryonic day 13.5 of the Jag1-cko model. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.3109/00016489.2012.690533
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 8
        StartPage: 1028
    Subjects:
      – SubjectFull: Inner ear
        Type: general
      – SubjectFull: Hair cells
        Type: general
      – SubjectFull: Sensitivity analysis
        Type: general
      – SubjectFull: Gene targeting
        Type: general
      – SubjectFull: Brain stem
        Type: general
      – SubjectFull: Gene expression
        Type: general
      – SubjectFull: Animal experimentation
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      – SubjectFull: Genetics
        Type: general
      – SubjectFull: Mammals
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      – SubjectFull: Mice
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      – SubjectFull: Proteins
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      – SubjectFull: Research funding
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      – SubjectFull: Data analysis software
        Type: general
      – SubjectFull: Mann Whitney U Test
        Type: general
    Titles:
      – TitleFull: Jagged1-mediated Notch signaling regulates mammalian inner ear development independent of lateral inhibition.
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              Text: Oct2012
              Type: published
              Y: 2012
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