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Distinctive patterns of alterations in proton efflux from goldfish retinal horizontal cells monitored with self-referencing H+-selective electrodes.

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Title: Distinctive patterns of alterations in proton efflux from goldfish retinal horizontal cells monitored with self-referencing H+-selective electrodes.
Authors: Kreitzer, Matthew A. (AUTHOR), Jacoby, Jason (AUTHOR), Naylor, Ethan (AUTHOR), Baker, Adam (AUTHOR), Grable, Trent (AUTHOR), Tran, Emma (AUTHOR), Booth, Sophie Erwin (AUTHOR), Qian, Haohua (AUTHOR), Malchow, Robert Paul (AUTHOR)
Source: European Journal of Neuroscience. Oct2012, Vol. 36 Issue 8, p3040-3050. 11p.
Subjects: Ion selective electrodes, Goldfish, Protons, Retina, Adenosine triphosphatase, Alkalinization, Hydrogen-ion concentration, Cell membranes
Abstract: The H+ hypothesis of lateral feedback inhibition in the outer retina predicts that depolarizing agents should increase H+ release from horizontal cells. To test this hypothesis, self-referencing H+-selective microelectrodes were used to measure extracellular H+ fluxes from isolated goldfish horizontal cells. We found a more complex pattern of cellular responses than previously observed from horizontal cells of other species examined using this technique. One class of cells had an initial standing signal indicative of high extracellular H+ adjacent to the cell membrane; challenge with glutamate, kainate or high extracellular potassium induced an extracellular alkalinization. This alkalinization was reduced by the calcium channel blockers nifedipine and cobalt. A second class of cells displayed spontaneous oscillations in extracellular H+ that were abolished by cobalt, nifedipine and low extracellular calcium. A strong correlation between changes in intracellular calcium and extracellular proton flux was detected in experiments simultaneously monitoring intracellular calcium and extracellular H+. A third set of cells was characterized by a standing extracellular alkalinization which was turned into an acidic signal by cobalt. In this last set of cells, addition of glutamate or high extracellular potassium did not significantly alter the proton signal. Taken together, the response characteristics of all three sets of neurons are most parsimoniously explained by activation of a plasma membrane Ca2+ ATPase pump, with an extracellular alkalinization resulting from exchange of intracellular calcium for extracellular H+. These findings argue strongly against the hypothesis that H+ release from horizontal cells mediates lateral inhibition in the outer retina. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Distinctive patterns of alterations in proton efflux from goldfish retinal horizontal cells monitored with self-referencing H<superscript>+</superscript>-selective electrodes.
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Oct2012, Vol. 36 Issue 8, p3040-3050. 11p.
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  Data: <searchLink fieldCode="DE" term="%22Ion+selective+electrodes%22">Ion selective electrodes</searchLink><br /><searchLink fieldCode="DE" term="%22Goldfish%22">Goldfish</searchLink><br /><searchLink fieldCode="DE" term="%22Protons%22">Protons</searchLink><br /><searchLink fieldCode="DE" term="%22Retina%22">Retina</searchLink><br /><searchLink fieldCode="DE" term="%22Adenosine+triphosphatase%22">Adenosine triphosphatase</searchLink><br /><searchLink fieldCode="DE" term="%22Alkalinization%22">Alkalinization</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrogen-ion+concentration%22">Hydrogen-ion concentration</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+membranes%22">Cell membranes</searchLink>
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  Label: Abstract
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  Data: The H+ hypothesis of lateral feedback inhibition in the outer retina predicts that depolarizing agents should increase H+ release from horizontal cells. To test this hypothesis, self-referencing H+-selective microelectrodes were used to measure extracellular H+ fluxes from isolated goldfish horizontal cells. We found a more complex pattern of cellular responses than previously observed from horizontal cells of other species examined using this technique. One class of cells had an initial standing signal indicative of high extracellular H+ adjacent to the cell membrane; challenge with glutamate, kainate or high extracellular potassium induced an extracellular alkalinization. This alkalinization was reduced by the calcium channel blockers nifedipine and cobalt. A second class of cells displayed spontaneous oscillations in extracellular H+ that were abolished by cobalt, nifedipine and low extracellular calcium. A strong correlation between changes in intracellular calcium and extracellular proton flux was detected in experiments simultaneously monitoring intracellular calcium and extracellular H+. A third set of cells was characterized by a standing extracellular alkalinization which was turned into an acidic signal by cobalt. In this last set of cells, addition of glutamate or high extracellular potassium did not significantly alter the proton signal. Taken together, the response characteristics of all three sets of neurons are most parsimoniously explained by activation of a plasma membrane Ca2+ ATPase pump, with an extracellular alkalinization resulting from exchange of intracellular calcium for extracellular H+. These findings argue strongly against the hypothesis that H+ release from horizontal cells mediates lateral inhibition in the outer retina. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/j.1460-9568.2012.08226.x
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      – Code: eng
        Text: English
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        StartPage: 3040
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      – SubjectFull: Ion selective electrodes
        Type: general
      – SubjectFull: Goldfish
        Type: general
      – SubjectFull: Protons
        Type: general
      – SubjectFull: Retina
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      – SubjectFull: Adenosine triphosphatase
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      – SubjectFull: Alkalinization
        Type: general
      – SubjectFull: Hydrogen-ion concentration
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      – SubjectFull: Cell membranes
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      – TitleFull: Distinctive patterns of alterations in proton efflux from goldfish retinal horizontal cells monitored with self-referencing H+-selective electrodes.
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              Text: Oct2012
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