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Major vault protein promotes locomotor recovery and regeneration after spinal cord injury in adult zebrafish.

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Title: Major vault protein promotes locomotor recovery and regeneration after spinal cord injury in adult zebrafish.
Authors: Pan, Hong‐Chao, Lin, Jin‐Fei, Ma, Li‐Ping, Shen, Yan‐Qin, Schachner, Melitta
Source: European Journal of Neuroscience. Jan2013, Vol. 37 Issue 2, p203-211. 9p. 1 Color Photograph, 5 Graphs.
Subjects: Spinal cord regeneration, Spinal cord injuries, Laboratory zebrafish, Major vault protein, Brain stem, Neural stem cells
Abstract: In contrast to mammals, adult zebrafish recover locomotor functions after spinal cord injury ( SCI), in part due to axonal regrowth and regeneration permissivity of the central nervous system. Upregulation of major vault protein ( MVP) expression after spinal cord injury in the brainstem of the adult zebrafish prompted us to probe for its contribution to recovery after SCI. MVP is a multifunctional protein expressed not only in many types of tumours but also in the nervous system, where its importance for regeneration is, however, unclear. Using an established zebrafish SCI model, we found that MVP mRNA and protein expression levels were increased in ependymal cells in the spinal cord caudal to the lesion site at 6 and 11 days after SCI. Double immunolabelling showed that MVP was co-localised with Islet-1 or tyrosine hydroxylase around the central canal of the spinal cord in sham-injured control fish and injured fish 11 days after surgery. MVP co-localised with the neural stem cell marker nestin in ependymal cells after injury. By using an in vivo morpholino-based knock-down approach, we found that the distance moved by MVP morpholino-treated fish was reduced at 4, 5 and 6 weeks after SCI when compared to fish treated with standard control morpholino. Knock-down of MVP resulted in reduced regrowth of axons from brainstem neurons into the spinal cord caudal to the lesion site. These results indicate that MVP supports locomotor recovery and axonal regrowth after SCI in adult zebrafish. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Major vault protein promotes locomotor recovery and regeneration after spinal cord injury in adult zebrafish.
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  Data: <searchLink fieldCode="AR" term="%22Pan%2C+Hong‐Chao%22">Pan, Hong‐Chao</searchLink><br /><searchLink fieldCode="AR" term="%22Lin%2C+Jin‐Fei%22">Lin, Jin‐Fei</searchLink><br /><searchLink fieldCode="AR" term="%22Ma%2C+Li‐Ping%22">Ma, Li‐Ping</searchLink><br /><searchLink fieldCode="AR" term="%22Shen%2C+Yan‐Qin%22">Shen, Yan‐Qin</searchLink><br /><searchLink fieldCode="AR" term="%22Schachner%2C+Melitta%22">Schachner, Melitta</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Jan2013, Vol. 37 Issue 2, p203-211. 9p. 1 Color Photograph, 5 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Spinal+cord+regeneration%22">Spinal cord regeneration</searchLink><br /><searchLink fieldCode="DE" term="%22Spinal+cord+injuries%22">Spinal cord injuries</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+zebrafish%22">Laboratory zebrafish</searchLink><br /><searchLink fieldCode="DE" term="%22Major+vault+protein%22">Major vault protein</searchLink><br /><searchLink fieldCode="DE" term="%22Brain+stem%22">Brain stem</searchLink><br /><searchLink fieldCode="DE" term="%22Neural+stem+cells%22">Neural stem cells</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: In contrast to mammals, adult zebrafish recover locomotor functions after spinal cord injury ( SCI), in part due to axonal regrowth and regeneration permissivity of the central nervous system. Upregulation of major vault protein ( MVP) expression after spinal cord injury in the brainstem of the adult zebrafish prompted us to probe for its contribution to recovery after SCI. MVP is a multifunctional protein expressed not only in many types of tumours but also in the nervous system, where its importance for regeneration is, however, unclear. Using an established zebrafish SCI model, we found that MVP mRNA and protein expression levels were increased in ependymal cells in the spinal cord caudal to the lesion site at 6 and 11 days after SCI. Double immunolabelling showed that MVP was co-localised with Islet-1 or tyrosine hydroxylase around the central canal of the spinal cord in sham-injured control fish and injured fish 11 days after surgery. MVP co-localised with the neural stem cell marker nestin in ependymal cells after injury. By using an in vivo morpholino-based knock-down approach, we found that the distance moved by MVP morpholino-treated fish was reduced at 4, 5 and 6 weeks after SCI when compared to fish treated with standard control morpholino. Knock-down of MVP resulted in reduced regrowth of axons from brainstem neurons into the spinal cord caudal to the lesion site. These results indicate that MVP supports locomotor recovery and axonal regrowth after SCI in adult zebrafish. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/ejn.12038
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        Text: English
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              Text: Jan2013
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