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A Localized Wnt Signal Orients Asymmetric Stem Cell Division in Vitro.

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Title: A Localized Wnt Signal Orients Asymmetric Stem Cell Division in Vitro.
Authors: Habib, Shukry J., Bi-Chang Chen, Feng-Chiao Tsai, Anastassiadis, Konstantinos, Meyer, Tobias, Betzig, Eric, Nusse, Roel
Source: Science (pre-March 2025). 3/22/2013, Vol. 339 Issue 6126, p1445-1448. 4p.
Subjects: Wnt proteins, Wnt protein genetics, Cell division, Embryonic stem cells, Stem cell culture, Research methodology, Catenins
Abstract: Developmental signals such as Wnts are often presented to cells in an oriented manner. To examine the consequences of local Wnt signaling, we immobilized Wnt proteins on beads and introduced them to embryonic stem cells in culture. At the single-cell level, the Wnt-bead induced asymmetric distribution of Wnt-β-catenin signaling components, oriented the plane of mitotic division, and directed asymmetric inheritance of centrosomes. Before cytokinesis was completed, the Wnt-proximal daughter cell expressed high levels of nuclear β-catenin and pluripotency genes, whereas the distal daughter cell acquired hallmarks of differentiation. We suggest that a spatially restricted Wnt signal induces an oriented cell division that generates distinct cell fates at predictable positions relative to the Wnt source. [ABSTRACT FROM AUTHOR]
Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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PubType: Academic Journal
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  Data: A Localized Wnt Signal Orients Asymmetric Stem Cell Division in Vitro.
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  Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 3/22/2013, Vol. 339 Issue 6126, p1445-1448. 4p.
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  Data: Developmental signals such as Wnts are often presented to cells in an oriented manner. To examine the consequences of local Wnt signaling, we immobilized Wnt proteins on beads and introduced them to embryonic stem cells in culture. At the single-cell level, the Wnt-bead induced asymmetric distribution of Wnt-β-catenin signaling components, oriented the plane of mitotic division, and directed asymmetric inheritance of centrosomes. Before cytokinesis was completed, the Wnt-proximal daughter cell expressed high levels of nuclear β-catenin and pluripotency genes, whereas the distal daughter cell acquired hallmarks of differentiation. We suggest that a spatially restricted Wnt signal induces an oriented cell division that generates distinct cell fates at predictable positions relative to the Wnt source. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=86688231
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        Value: 10.1126/science.1231077
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        Text: English
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      – SubjectFull: Wnt proteins
        Type: general
      – SubjectFull: Wnt protein genetics
        Type: general
      – SubjectFull: Cell division
        Type: general
      – SubjectFull: Embryonic stem cells
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      – SubjectFull: Stem cell culture
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      – SubjectFull: Catenins
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            NameFull: Meyer, Tobias
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              Text: 3/22/2013
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