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Characterization of the expression of PDZ-RhoGEF, LARG and Gα12 /Gα13 proteins in the murine nervous system.

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Title: Characterization of the expression of PDZ-RhoGEF, LARG and Gα12 /Gα13 proteins in the murine nervous system.
Authors: Kuner, R. (AUTHOR), Swiercz, J. M. (AUTHOR), Zywietz, A. (AUTHOR), Tappe, A. (AUTHOR), Offermanns, S. (AUTHOR)
Source: European Journal of Neuroscience. Dec2002, Vol. 16 Issue 12, p2333-2341. 9p.
Subjects: Rho(D) immune globulin, G proteins, Nervous system
Abstract: Abstract Small GTPases of the Rho-family, like Rho, Rac and Cdc42, are involved in neuronal morphogenesis by regulating growth cone morphology or dendritic spine formation. G-proteins of the G12 -family, G12 and G13 , couple G-protein-coupled receptors (GPCRs) to the activation of RhoA. Recently, two novel Rho-specific guanine nucleotide exchange factors (RhoGEFs), PDZ-RhoGEF and LARG, have been identified to interact with the activated α-subunits of G12 /G13 and are thus believed to mediate GPCR-induced Rho activation. Although studies in neuronal cell lines have shown that G12 /G13 and PDZ-RhoGEF mediate GPCR-induced neurite retraction, the role, as well as the expression of this signalling pathway, in intact brain has not been adequately studied. In the present study, we have characterized systematically the expression of Gα12 , Gα13 , PDZ-RhoGEF and LARG in various murine tissues as well as their subcellular localization in the central and peripheral nervous systems. By performing immunohistochemistry, using polyclonal antibodies raised against the above proteins, we observed that Gα12 , Gα13 and their RhoGEF-effectors are distributed widely in the mammalian nervous system. Moreover, these proteins localize to distinct morphological compartments within neurons. While LARG and Gα12 were mainly found in somata of the neurons, PDZ-RhoGEF and Gα13 were predominantly localized in the neuropil of central neurons. Interestingly, PDZ-RhoGEF is a neural-specific protein, whereas LARG is nearly ubiqoutous. Our data provide evidence that the G12/13 –RhoGEF-mediated pathway is present throughout the adult brain and may be involved in regulation of neuronal morphogenesis and function via GPCRs. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Characterization of the expression of PDZ-RhoGEF, LARG and Gα<subscript>12</subscript> /Gα<subscript>13</subscript> proteins in the murine nervous system.
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  Data: <searchLink fieldCode="AR" term="%22Kuner%2C+R%2E%22">Kuner, R.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Swiercz%2C+J%2E+M%2E%22">Swiercz, J. M.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zywietz%2C+A%2E%22">Zywietz, A.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tappe%2C+A%2E%22">Tappe, A.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Offermanns%2C+S%2E%22">Offermanns, S.</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Dec2002, Vol. 16 Issue 12, p2333-2341. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Rho%28D%29+immune+globulin%22">Rho(D) immune globulin</searchLink><br /><searchLink fieldCode="DE" term="%22G+proteins%22">G proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Nervous+system%22">Nervous system</searchLink>
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  Label: Abstract
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  Data: Abstract Small GTPases of the Rho-family, like Rho, Rac and Cdc42, are involved in neuronal morphogenesis by regulating growth cone morphology or dendritic spine formation. G-proteins of the G12 -family, G12 and G13 , couple G-protein-coupled receptors (GPCRs) to the activation of RhoA. Recently, two novel Rho-specific guanine nucleotide exchange factors (RhoGEFs), PDZ-RhoGEF and LARG, have been identified to interact with the activated α-subunits of G12 /G13 and are thus believed to mediate GPCR-induced Rho activation. Although studies in neuronal cell lines have shown that G12 /G13 and PDZ-RhoGEF mediate GPCR-induced neurite retraction, the role, as well as the expression of this signalling pathway, in intact brain has not been adequately studied. In the present study, we have characterized systematically the expression of Gα12 , Gα13 , PDZ-RhoGEF and LARG in various murine tissues as well as their subcellular localization in the central and peripheral nervous systems. By performing immunohistochemistry, using polyclonal antibodies raised against the above proteins, we observed that Gα12 , Gα13 and their RhoGEF-effectors are distributed widely in the mammalian nervous system. Moreover, these proteins localize to distinct morphological compartments within neurons. While LARG and Gα12 were mainly found in somata of the neurons, PDZ-RhoGEF and Gα13 were predominantly localized in the neuropil of central neurons. Interestingly, PDZ-RhoGEF is a neural-specific protein, whereas LARG is nearly ubiqoutous. Our data provide evidence that the G12/13 –RhoGEF-mediated pathway is present throughout the adult brain and may be involved in regulation of neuronal morphogenesis and function via GPCRs. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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              Text: Dec2002
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