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Characterization of ion currents of murine CD117pos stem cells in vitro and their modulation under AT2R stimulation.

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Title: Characterization of ion currents of murine CD117pos stem cells in vitro and their modulation under AT2R stimulation.
Authors: Ludwig, M., Skorska, A., Tölk, A., Hopp, H.‐H., Patejdl, R., Li, J., Steinhoff, G., Noack, T.
Source: Acta Physiologica. Jul2013, Vol. 208 Issue 3, p274-287. 14p. 1 Color Photograph, 8 Graphs.
Subjects: Hematopoietic stem cells, C-kit protein, Cardiac regeneration, Angiotensin II, Angiotensin receptors, Reverse transcriptase polymerase chain reaction
Abstract: Aim Hematopoietic stem cells, especially CD117pos cells, have been found to possess a regenerative potential in various tissues, in particular cardiac muscle. However, the characterization of the relevant ion currents of stem cells prior to implantation lacks documentation. Activation of angiotensin II type 2 receptor ( AT2R) can lead to further cell differentiation and receptor auto-expression and might thus influence electrophysiological properties of CD117pos stem cells. This study was designed to functionally characterize membrane currents of CD117pos cells under normal and AT2R-stimulated conditions. Methods CD117pos murine bone marrow stem cells were isolated with MACS technique and stimulated for the AT2R with angiotensin II and losartan for 3-5 days prior to patch-clamp measurements. RT- PCR was used to determine channel expression. Endothelial properties were analysed with immunocytochemistry and ac LDL uptake assay. Results A well-expressed inward rectifying current ( IKir) was identified in cultured CD117pos cells. Furthermore, a ZD 7288 ( HCN channel blocker)-sensitive current component was isolated. Voltage-dependent potassium currents and chloride currents were less expressed. A small fraction of cells demonstrated voltage- and time-dependent inward currents. In AT2R-stimulated cells inward rectifying the hyperpolarization-induced inward currents were slightly attenuated on the translational level but showed increased mRNA expression. Cultured CD117pos cells express CD31 and VEGFR-2 and significantly increased the uptake of ac LDL. Conclusions CD117pos cells do not have properties of action potential-generating cells and moderately change their excitability during AT2R stimulation. Electrophysiological and molecular properties of control and AT2R-stimulated cells point to a differentiation to vascular endothelial cells. This could increase beneficial vascularization in injured tissues. [ABSTRACT FROM AUTHOR]
Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Characterization of ion currents of murine CD117<superscript>pos</superscript> stem cells in vitro and their modulation under AT<subscript>2</subscript>R stimulation.
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  Data: <searchLink fieldCode="AR" term="%22Ludwig%2C+M%2E%22">Ludwig, M.</searchLink><br /><searchLink fieldCode="AR" term="%22Skorska%2C+A%2E%22">Skorska, A.</searchLink><br /><searchLink fieldCode="AR" term="%22Tölk%2C+A%2E%22">Tölk, A.</searchLink><br /><searchLink fieldCode="AR" term="%22Hopp%2C+H%2E‐H%2E%22">Hopp, H.‐H.</searchLink><br /><searchLink fieldCode="AR" term="%22Patejdl%2C+R%2E%22">Patejdl, R.</searchLink><br /><searchLink fieldCode="AR" term="%22Li%2C+J%2E%22">Li, J.</searchLink><br /><searchLink fieldCode="AR" term="%22Steinhoff%2C+G%2E%22">Steinhoff, G.</searchLink><br /><searchLink fieldCode="AR" term="%22Noack%2C+T%2E%22">Noack, T.</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Acta+Physiologica%22">Acta Physiologica</searchLink>. Jul2013, Vol. 208 Issue 3, p274-287. 14p. 1 Color Photograph, 8 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Hematopoietic+stem+cells%22">Hematopoietic stem cells</searchLink><br /><searchLink fieldCode="DE" term="%22C-kit+protein%22">C-kit protein</searchLink><br /><searchLink fieldCode="DE" term="%22Cardiac+regeneration%22">Cardiac regeneration</searchLink><br /><searchLink fieldCode="DE" term="%22Angiotensin+II%22">Angiotensin II</searchLink><br /><searchLink fieldCode="DE" term="%22Angiotensin+receptors%22">Angiotensin receptors</searchLink><br /><searchLink fieldCode="DE" term="%22Reverse+transcriptase+polymerase+chain+reaction%22">Reverse transcriptase polymerase chain reaction</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Aim Hematopoietic stem cells, especially CD117pos cells, have been found to possess a regenerative potential in various tissues, in particular cardiac muscle. However, the characterization of the relevant ion currents of stem cells prior to implantation lacks documentation. Activation of angiotensin II type 2 receptor ( AT2R) can lead to further cell differentiation and receptor auto-expression and might thus influence electrophysiological properties of CD117pos stem cells. This study was designed to functionally characterize membrane currents of CD117pos cells under normal and AT2R-stimulated conditions. Methods CD117pos murine bone marrow stem cells were isolated with MACS technique and stimulated for the AT2R with angiotensin II and losartan for 3-5 days prior to patch-clamp measurements. RT- PCR was used to determine channel expression. Endothelial properties were analysed with immunocytochemistry and ac LDL uptake assay. Results A well-expressed inward rectifying current ( IKir) was identified in cultured CD117pos cells. Furthermore, a ZD 7288 ( HCN channel blocker)-sensitive current component was isolated. Voltage-dependent potassium currents and chloride currents were less expressed. A small fraction of cells demonstrated voltage- and time-dependent inward currents. In AT2R-stimulated cells inward rectifying the hyperpolarization-induced inward currents were slightly attenuated on the translational level but showed increased mRNA expression. Cultured CD117pos cells express CD31 and VEGFR-2 and significantly increased the uptake of ac LDL. Conclusions CD117pos cells do not have properties of action potential-generating cells and moderately change their excitability during AT2R stimulation. Electrophysiological and molecular properties of control and AT2R-stimulated cells point to a differentiation to vascular endothelial cells. This could increase beneficial vascularization in injured tissues. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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              Text: Jul2013
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