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Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications.

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Title: Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications.
Authors: Sato, Toshiro, Clevers, Hans
Source: Science (pre-March 2025). 6/7/2013, Vol. 340 Issue 6137, p1190-1194. 5p.
Subjects: Stem cells, Intestinal development, Epithelium, Morphogenesis, Organ culture, Protein receptors, Small intestine physiology, Regenerative medicine, Tissues -- Models
Abstract: Recent examples have highlighted how stem cells have the capability to initiate morphogenesis in vitro; that is, to generate complex structures in culture that closely parallel their in vivo counterparts. Lgr5, the receptor for the Wnt-agonistic R-spondins, marks stem cells in multiple adult organs of mice and humans. In R-spondin-based three-dimensional cultures, these Lgr5 stem cells can grow into ever-expanding epithelial organoids that retain their original organ identity. Single Lgr5 stem cells derived from the intestine can be cultured to build epithelial structures that retain hallmarks of the in vivo epithelium. Here, we review the mechanisms that support this notable example of self-organization and discuss applications of this technology for stem cell research, disease modeling (e.g., for colorectal cancer and cystic fibrosis), and regenerative medicine. [ABSTRACT FROM AUTHOR]
Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications.
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  Data: <searchLink fieldCode="AR" term="%22Sato%2C+Toshiro%22">Sato, Toshiro</searchLink><br /><searchLink fieldCode="AR" term="%22Clevers%2C+Hans%22">Clevers, Hans</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 6/7/2013, Vol. 340 Issue 6137, p1190-1194. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Stem+cells%22">Stem cells</searchLink><br /><searchLink fieldCode="DE" term="%22Intestinal+development%22">Intestinal development</searchLink><br /><searchLink fieldCode="DE" term="%22Epithelium%22">Epithelium</searchLink><br /><searchLink fieldCode="DE" term="%22Morphogenesis%22">Morphogenesis</searchLink><br /><searchLink fieldCode="DE" term="%22Organ+culture%22">Organ culture</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+receptors%22">Protein receptors</searchLink><br /><searchLink fieldCode="DE" term="%22Small+intestine+physiology%22">Small intestine physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Regenerative+medicine%22">Regenerative medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Tissues+--+Models%22">Tissues -- Models</searchLink>
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  Data: Recent examples have highlighted how stem cells have the capability to initiate morphogenesis in vitro; that is, to generate complex structures in culture that closely parallel their in vivo counterparts. Lgr5, the receptor for the Wnt-agonistic R-spondins, marks stem cells in multiple adult organs of mice and humans. In R-spondin-based three-dimensional cultures, these Lgr5 stem cells can grow into ever-expanding epithelial organoids that retain their original organ identity. Single Lgr5 stem cells derived from the intestine can be cultured to build epithelial structures that retain hallmarks of the in vivo epithelium. Here, we review the mechanisms that support this notable example of self-organization and discuss applications of this technology for stem cell research, disease modeling (e.g., for colorectal cancer and cystic fibrosis), and regenerative medicine. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=88240122
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        Value: 10.1126/science.1234852
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        Text: English
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      – SubjectFull: Stem cells
        Type: general
      – SubjectFull: Intestinal development
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      – SubjectFull: Epithelium
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      – SubjectFull: Morphogenesis
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      – SubjectFull: Organ culture
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      – SubjectFull: Protein receptors
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      – SubjectFull: Small intestine physiology
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      – SubjectFull: Regenerative medicine
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              Text: 6/7/2013
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              Y: 2013
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