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Structural insight into magnetochrome-mediated magnetite biomineralization.

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Title: Structural insight into magnetochrome-mediated magnetite biomineralization.
Authors: Siponen, Marina I., Legrand, Pierre, Widdrat, Marc, Jones, Stephanie R., Zhang, Wei-Jia, Chang, Michelle C. Y., Faivre, Damien, Arnoux, Pascal, Pignol, David
Source: Nature. 10/31/2013, Vol. 502 Issue 7473, p681-684. 4p. 4 Diagrams.
Subjects: Magnetite, Biomineralization, Magnetotactic bacteria, Magnetosomes, Crystal structure, Bacterial pigments
Abstract: Magnetotactic bacteria align along the Earth's magnetic field using an organelle called the magnetosome, a biomineralized magnetite (Fe(ii)Fe(iii)2O4) or greigite (Fe(ii)Fe(iii)2S4) crystal embedded in a lipid vesicle. Although the need for both iron(ii) and iron(iii) is clear, little is known about the biological mechanisms controlling their ratio. Here we present the structure of the magnetosome-associated protein MamP and find that it is built on a unique arrangement of a self-plugged PDZ domain fused to two magnetochrome domains, defining a new class of c-type cytochrome exclusively found in magnetotactic bacteria. Mutational analysis, enzyme kinetics, co-crystallization with iron(ii) and an in vitro MamP-assisted magnetite production assay establish MamP as an iron oxidase that contributes to the formation of iron(iii) ferrihydrite eventually required for magnetite crystal growth in vivo. These results demonstrate the molecular mechanisms of iron management taking place inside the magnetosome and highlight the role of magnetochrome in iron biomineralization. [ABSTRACT FROM AUTHOR]
Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Structural insight into magnetochrome-mediated magnetite biomineralization.
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  Data: <searchLink fieldCode="AR" term="%22Siponen%2C+Marina+I%2E%22">Siponen, Marina I.</searchLink><br /><searchLink fieldCode="AR" term="%22Legrand%2C+Pierre%22">Legrand, Pierre</searchLink><br /><searchLink fieldCode="AR" term="%22Widdrat%2C+Marc%22">Widdrat, Marc</searchLink><br /><searchLink fieldCode="AR" term="%22Jones%2C+Stephanie+R%2E%22">Jones, Stephanie R.</searchLink><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Wei-Jia%22">Zhang, Wei-Jia</searchLink><br /><searchLink fieldCode="AR" term="%22Chang%2C+Michelle+C%2E+Y%2E%22">Chang, Michelle C. Y.</searchLink><br /><searchLink fieldCode="AR" term="%22Faivre%2C+Damien%22">Faivre, Damien</searchLink><br /><searchLink fieldCode="AR" term="%22Arnoux%2C+Pascal%22">Arnoux, Pascal</searchLink><br /><searchLink fieldCode="AR" term="%22Pignol%2C+David%22">Pignol, David</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Nature%22">Nature</searchLink>. 10/31/2013, Vol. 502 Issue 7473, p681-684. 4p. 4 Diagrams.
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  Data: <searchLink fieldCode="DE" term="%22Magnetite%22">Magnetite</searchLink><br /><searchLink fieldCode="DE" term="%22Biomineralization%22">Biomineralization</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetotactic+bacteria%22">Magnetotactic bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetosomes%22">Magnetosomes</searchLink><br /><searchLink fieldCode="DE" term="%22Crystal+structure%22">Crystal structure</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+pigments%22">Bacterial pigments</searchLink>
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  Data: Magnetotactic bacteria align along the Earth's magnetic field using an organelle called the magnetosome, a biomineralized magnetite (Fe(ii)Fe(iii)2O4) or greigite (Fe(ii)Fe(iii)2S4) crystal embedded in a lipid vesicle. Although the need for both iron(ii) and iron(iii) is clear, little is known about the biological mechanisms controlling their ratio. Here we present the structure of the magnetosome-associated protein MamP and find that it is built on a unique arrangement of a self-plugged PDZ domain fused to two magnetochrome domains, defining a new class of c-type cytochrome exclusively found in magnetotactic bacteria. Mutational analysis, enzyme kinetics, co-crystallization with iron(ii) and an in vitro MamP-assisted magnetite production assay establish MamP as an iron oxidase that contributes to the formation of iron(iii) ferrihydrite eventually required for magnetite crystal growth in vivo. These results demonstrate the molecular mechanisms of iron management taking place inside the magnetosome and highlight the role of magnetochrome in iron biomineralization. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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