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Neuroprotective Effects of Lycium chinense Miller Against Rotenone-Induced Neurotoxicity in PC12 Cells.

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Title: Neuroprotective Effects of Lycium chinense Miller Against Rotenone-Induced Neurotoxicity in PC12 Cells.
Authors: Im, A-Rang, Kim, Young-Hwa, Uddin, Md Romij, Chae, Sungwook, Lee, Hye Won, Kim, Yeong Shik, Lee, Mi Young
Source: American Journal of Chinese Medicine. 2013, Vol. 41 Issue 6, p1343-1359. 17p. 4 Color Photographs, 9 Black and White Photographs.
Subjects: Analysis of variance, Animal experimentation, Apoptosis, Cell culture, Cell physiology, Flow cytometry, Mitochondria, Neurodegeneration, Rats, Research funding, T-test (Statistics), Western immunoblotting, Plant extracts, Isoflavones, Neuroprotective agents, Data analysis software, Descriptive statistics
Abstract: Rotenone, an inhibitor of mitochondrial complex I, has been widely regarded as a neurotoxin because it induces a Parkinson's disease-like syndrome. The fruit and root bark of Lycium chinense Miller have been used as traditional medicines in Asia to treat neurodegenerative diseases. In this study, we examined the neuroprotective effects of Lycium chinense Miller extracts in rotenone-treated PC12 cells. Treatment with rotenone reduced PC12 cell viability and cellular ATP levels. Conversely, caspase 3/7 activity, the ratio of Bax:Bcl-2 expression levels, mitochondrial superoxide level, and intracellular calcium (Ca2+) concentration were elevated. Pretreatment with Lycium chinense Miller extracts significantly increased cell viability and ATP levels. Additionally, they attenuated caspase activation, mitochondrial membrane depolarization and mitochondrial superoxide production. Moreover, confocal microscopy showed that the mitochondrial staining pattern was restored from that of extracts treated cells and that the increase in intracellular 2+ level was blunted by treatment with the extracts. Our results suggest that Lycium chinense Miller extracts may have the possible beneficial effects in Parkinson's disease by attenuating rotenone induced toxicity. [ABSTRACT FROM AUTHOR]
Copyright of American Journal of Chinese Medicine is the property of World Scientific Publishing Company and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Neuroprotective Effects of Lycium chinense Miller Against Rotenone-Induced Neurotoxicity in PC12 Cells.
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  Data: <searchLink fieldCode="AR" term="%22Im%2C+A-Rang%22">Im, A-Rang</searchLink><br /><searchLink fieldCode="AR" term="%22Kim%2C+Young-Hwa%22">Kim, Young-Hwa</searchLink><br /><searchLink fieldCode="AR" term="%22Uddin%2C+Md+Romij%22">Uddin, Md Romij</searchLink><br /><searchLink fieldCode="AR" term="%22Chae%2C+Sungwook%22">Chae, Sungwook</searchLink><br /><searchLink fieldCode="AR" term="%22Lee%2C+Hye+Won%22">Lee, Hye Won</searchLink><br /><searchLink fieldCode="AR" term="%22Kim%2C+Yeong+Shik%22">Kim, Yeong Shik</searchLink><br /><searchLink fieldCode="AR" term="%22Lee%2C+Mi+Young%22">Lee, Mi Young</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22American+Journal+of+Chinese+Medicine%22">American Journal of Chinese Medicine</searchLink>. 2013, Vol. 41 Issue 6, p1343-1359. 17p. 4 Color Photographs, 9 Black and White Photographs.
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  Data: <searchLink fieldCode="DE" term="%22Analysis+of+variance%22">Analysis of variance</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+experimentation%22">Animal experimentation</searchLink><br /><searchLink fieldCode="DE" term="%22Apoptosis%22">Apoptosis</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+culture%22">Cell culture</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+physiology%22">Cell physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Flow+cytometry%22">Flow cytometry</searchLink><br /><searchLink fieldCode="DE" term="%22Mitochondria%22">Mitochondria</searchLink><br /><searchLink fieldCode="DE" term="%22Neurodegeneration%22">Neurodegeneration</searchLink><br /><searchLink fieldCode="DE" term="%22Rats%22">Rats</searchLink><br /><searchLink fieldCode="DE" term="%22Research+funding%22">Research funding</searchLink><br /><searchLink fieldCode="DE" term="%22T-test+%28Statistics%29%22">T-test (Statistics)</searchLink><br /><searchLink fieldCode="DE" term="%22Western+immunoblotting%22">Western immunoblotting</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+extracts%22">Plant extracts</searchLink><br /><searchLink fieldCode="DE" term="%22Isoflavones%22">Isoflavones</searchLink><br /><searchLink fieldCode="DE" term="%22Neuroprotective+agents%22">Neuroprotective agents</searchLink><br /><searchLink fieldCode="DE" term="%22Data+analysis+software%22">Data analysis software</searchLink><br /><searchLink fieldCode="DE" term="%22Descriptive+statistics%22">Descriptive statistics</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Rotenone, an inhibitor of mitochondrial complex I, has been widely regarded as a neurotoxin because it induces a Parkinson's disease-like syndrome. The fruit and root bark of Lycium chinense Miller have been used as traditional medicines in Asia to treat neurodegenerative diseases. In this study, we examined the neuroprotective effects of Lycium chinense Miller extracts in rotenone-treated PC12 cells. Treatment with rotenone reduced PC12 cell viability and cellular ATP levels. Conversely, caspase 3/7 activity, the ratio of Bax:Bcl-2 expression levels, mitochondrial superoxide level, and intracellular calcium (Ca2+) concentration were elevated. Pretreatment with Lycium chinense Miller extracts significantly increased cell viability and ATP levels. Additionally, they attenuated caspase activation, mitochondrial membrane depolarization and mitochondrial superoxide production. Moreover, confocal microscopy showed that the mitochondrial staining pattern was restored from that of extracts treated cells and that the increase in intracellular 2+ level was blunted by treatment with the extracts. Our results suggest that Lycium chinense Miller extracts may have the possible beneficial effects in Parkinson's disease by attenuating rotenone induced toxicity. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of American Journal of Chinese Medicine is the property of World Scientific Publishing Company and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1142/S0192415X13500900
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 17
        StartPage: 1343
    Subjects:
      – SubjectFull: Analysis of variance
        Type: general
      – SubjectFull: Animal experimentation
        Type: general
      – SubjectFull: Apoptosis
        Type: general
      – SubjectFull: Cell culture
        Type: general
      – SubjectFull: Cell physiology
        Type: general
      – SubjectFull: Flow cytometry
        Type: general
      – SubjectFull: Mitochondria
        Type: general
      – SubjectFull: Neurodegeneration
        Type: general
      – SubjectFull: Rats
        Type: general
      – SubjectFull: Research funding
        Type: general
      – SubjectFull: T-test (Statistics)
        Type: general
      – SubjectFull: Western immunoblotting
        Type: general
      – SubjectFull: Plant extracts
        Type: general
      – SubjectFull: Isoflavones
        Type: general
      – SubjectFull: Neuroprotective agents
        Type: general
      – SubjectFull: Data analysis software
        Type: general
      – SubjectFull: Descriptive statistics
        Type: general
    Titles:
      – TitleFull: Neuroprotective Effects of Lycium chinense Miller Against Rotenone-Induced Neurotoxicity in PC12 Cells.
        Type: main
  BibRelationships:
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            NameFull: Im, A-Rang
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            NameFull: Kim, Young-Hwa
      – PersonEntity:
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            NameFull: Uddin, Md Romij
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            NameFull: Chae, Sungwook
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            NameFull: Lee, Hye Won
      – PersonEntity:
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            NameFull: Kim, Yeong Shik
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            NameFull: Lee, Mi Young
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            – D: 01
              M: 12
              Text: 2013
              Type: published
              Y: 2013
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              Value: 0192415X
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              Value: 41
            – Type: issue
              Value: 6
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            – TitleFull: American Journal of Chinese Medicine
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