Antagonism of brain insulin-like growth factor-1 receptors blocks estradiol effects on memory and levels of hippocampal synaptic proteins in ovariectomized rats.
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| Title: | Antagonism of brain insulin-like growth factor-1 receptors blocks estradiol effects on memory and levels of hippocampal synaptic proteins in ovariectomized rats. |
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| Authors: | Nelson, Britta, Springer, Rachel, Daniel, Jill |
| Source: | Psychopharmacology. Mar2014, Vol. 231 Issue 5, p899-907. 9p. 4 Graphs. |
| Subjects: | Psychopharmacological research, Laboratory rats, Estradiol, Memory research, Hippocampus (Brain), Postsynaptic density protein, Somatomedin C, Insulin-like growth factor receptors |
| Abstract: | Rationale: Treatment with estradiol, the primary estrogen produced by the ovaries, enhances hippocampus-dependent spatial memory and increases levels of hippocampal synaptic proteins in ovariectomized rats. Increasing evidence indicates that the ability of estradiol to impact the brain and behavior is dependent upon its interaction with insulin-like growth factor-1 (IGF-1). Objective: The goal of the current experiment was to test the hypothesis that the ability of estradiol to impact hippocampus-dependent memory and levels of hippocampal synaptic proteins is dependent on its interaction with IGF-1. Methods: Adult rats were ovariectomized and implanted with estradiol or control capsules and trained on a radial-maze spatial memory task. After training, rats were implanted with intracerebroventricular cannulae attached to osmotic minipumps (flow rate 0.15 μl/h). Half of each hormone treatment group received continuous delivery of JB1 (300 μg/ml), an IGF-1 receptor antagonist, and half received delivery of aCSF vehicle. Rats were tested on trials in the radial-arm maze during which delays were imposed between the fourth and fifth arm choices. Hippocampal levels of synaptic proteins were measured by western blotting. Results: Estradiol treatment resulted in significantly enhanced memory. JB1 blocked that enhancement. Estradiol treatment resulted in significantly increased hippocampal levels of postsynaptic density protein 95 (PSD-95), spinophilin, and synaptophysin. JB1 blocked the estradiol-induced increase of PSD-95 and spinophilin and attenuated the increase of synaptophysin. Conclusions: Results support a role for IGF-1 receptor activity in estradiol-induced enhancement of spatial memory that may be dependent on changes in synapse structure in the hippocampus brought upon by estradiol/IGF-1 interactions. [ABSTRACT FROM AUTHOR] |
| Copyright of Psychopharmacology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 94397463 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Antagonism of brain insulin-like growth factor-1 receptors blocks estradiol effects on memory and levels of hippocampal synaptic proteins in ovariectomized rats. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Nelson%2C+Britta%22">Nelson, Britta</searchLink><br /><searchLink fieldCode="AR" term="%22Springer%2C+Rachel%22">Springer, Rachel</searchLink><br /><searchLink fieldCode="AR" term="%22Daniel%2C+Jill%22">Daniel, Jill</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Psychopharmacology%22">Psychopharmacology</searchLink>. Mar2014, Vol. 231 Issue 5, p899-907. 9p. 4 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Psychopharmacological+research%22">Psychopharmacological research</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+rats%22">Laboratory rats</searchLink><br /><searchLink fieldCode="DE" term="%22Estradiol%22">Estradiol</searchLink><br /><searchLink fieldCode="DE" term="%22Memory+research%22">Memory research</searchLink><br /><searchLink fieldCode="DE" term="%22Hippocampus+%28Brain%29%22">Hippocampus (Brain)</searchLink><br /><searchLink fieldCode="DE" term="%22Postsynaptic+density+protein%22">Postsynaptic density protein</searchLink><br /><searchLink fieldCode="DE" term="%22Somatomedin+C%22">Somatomedin C</searchLink><br /><searchLink fieldCode="DE" term="%22Insulin-like+growth+factor+receptors%22">Insulin-like growth factor receptors</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Rationale: Treatment with estradiol, the primary estrogen produced by the ovaries, enhances hippocampus-dependent spatial memory and increases levels of hippocampal synaptic proteins in ovariectomized rats. Increasing evidence indicates that the ability of estradiol to impact the brain and behavior is dependent upon its interaction with insulin-like growth factor-1 (IGF-1). Objective: The goal of the current experiment was to test the hypothesis that the ability of estradiol to impact hippocampus-dependent memory and levels of hippocampal synaptic proteins is dependent on its interaction with IGF-1. Methods: Adult rats were ovariectomized and implanted with estradiol or control capsules and trained on a radial-maze spatial memory task. After training, rats were implanted with intracerebroventricular cannulae attached to osmotic minipumps (flow rate 0.15 μl/h). Half of each hormone treatment group received continuous delivery of JB1 (300 μg/ml), an IGF-1 receptor antagonist, and half received delivery of aCSF vehicle. Rats were tested on trials in the radial-arm maze during which delays were imposed between the fourth and fifth arm choices. Hippocampal levels of synaptic proteins were measured by western blotting. Results: Estradiol treatment resulted in significantly enhanced memory. JB1 blocked that enhancement. Estradiol treatment resulted in significantly increased hippocampal levels of postsynaptic density protein 95 (PSD-95), spinophilin, and synaptophysin. JB1 blocked the estradiol-induced increase of PSD-95 and spinophilin and attenuated the increase of synaptophysin. Conclusions: Results support a role for IGF-1 receptor activity in estradiol-induced enhancement of spatial memory that may be dependent on changes in synapse structure in the hippocampus brought upon by estradiol/IGF-1 interactions. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Psychopharmacology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00213-013-3310-7 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 899 Subjects: – SubjectFull: Psychopharmacological research Type: general – SubjectFull: Laboratory rats Type: general – SubjectFull: Estradiol Type: general – SubjectFull: Memory research Type: general – SubjectFull: Hippocampus (Brain) Type: general – SubjectFull: Postsynaptic density protein Type: general – SubjectFull: Somatomedin C Type: general – SubjectFull: Insulin-like growth factor receptors Type: general Titles: – TitleFull: Antagonism of brain insulin-like growth factor-1 receptors blocks estradiol effects on memory and levels of hippocampal synaptic proteins in ovariectomized rats. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Nelson, Britta – PersonEntity: Name: NameFull: Springer, Rachel – PersonEntity: Name: NameFull: Daniel, Jill IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 00333158 Numbering: – Type: volume Value: 231 – Type: issue Value: 5 Titles: – TitleFull: Psychopharmacology Type: main |
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