Targeting neurons and photons for optogenetics.

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Title:	Targeting neurons and photons for optogenetics.
Authors:	Packer, Adam M, Roska, Botond, Häusser, Michael
Source:	Nature Neuroscience. Jul2013, Vol. 16 Issue 7, p805-815. 11p.
Subjects:	Optogenetics, Neurosciences, Neuron analysis, Imaging systems, Gene expression
Abstract:	Optogenetic approaches promise to revolutionize neuroscience by using light to manipulate neural activity in genetically or functionally defined neurons with millisecond precision. Harnessing the full potential of optogenetic tools, however, requires light to be targeted to the right neurons at the right time. Here we discuss some barriers and potential solutions to this problem. We review methods for targeting the expression of light-activatable molecules to specific cell types, under genetic, viral or activity-dependent control. Next we explore new ways to target light to individual neurons to allow their precise activation and inactivation. These techniques provide a precision in the temporal and spatial activation of neurons that was not achievable in previous experiments. In combination with simultaneous recording and imaging techniques, these strategies will allow us to mimic the natural activity patterns of neurons in vivo, enabling previously impossible 'dream experiments'. [ABSTRACT FROM AUTHOR]
	Copyright of Nature Neuroscience is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database:	Psychology and Behavioral Sciences Collection

FullText	Links: – Type: pdflink Text: Availability: 0
Header	DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 95677796 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0
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Items	– Name: Title Label: Title Group: Ti Data: Targeting neurons and photons for optogenetics. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Packer%2C+Adam+M%22">Packer, Adam M</searchLink><br /><searchLink fieldCode="AR" term="%22Roska%2C+Botond%22">Roska, Botond</searchLink><br /><searchLink fieldCode="AR" term="%22Häusser%2C+Michael%22">Häusser, Michael</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Nature+Neuroscience%22">Nature Neuroscience</searchLink>. Jul2013, Vol. 16 Issue 7, p805-815. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Optogenetics%22">Optogenetics</searchLink><br /><searchLink fieldCode="DE" term="%22Neurosciences%22">Neurosciences</searchLink><br /><searchLink fieldCode="DE" term="%22Neuron+analysis%22">Neuron analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Imaging+systems%22">Imaging systems</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Optogenetic approaches promise to revolutionize neuroscience by using light to manipulate neural activity in genetically or functionally defined neurons with millisecond precision. Harnessing the full potential of optogenetic tools, however, requires light to be targeted to the right neurons at the right time. Here we discuss some barriers and potential solutions to this problem. We review methods for targeting the expression of light-activatable molecules to specific cell types, under genetic, viral or activity-dependent control. Next we explore new ways to target light to individual neurons to allow their precise activation and inactivation. These techniques provide a precision in the temporal and spatial activation of neurons that was not achievable in previous experiments. In combination with simultaneous recording and imaging techniques, these strategies will allow us to mimic the natural activity patterns of neurons in vivo, enabling previously impossible 'dream experiments'. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Nature Neuroscience is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo	BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1038/nn.3427 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 805 Subjects: – SubjectFull: Optogenetics Type: general – SubjectFull: Neurosciences Type: general – SubjectFull: Neuron analysis Type: general – SubjectFull: Imaging systems Type: general – SubjectFull: Gene expression Type: general Titles: – TitleFull: Targeting neurons and photons for optogenetics. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Packer, Adam M – PersonEntity: Name: NameFull: Roska, Botond – PersonEntity: Name: NameFull: Häusser, Michael IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: Jul2013 Type: published Y: 2013 Identifiers: – Type: issn-print Value: 10976256 Numbering: – Type: volume Value: 16 – Type: issue Value: 7 Titles: – TitleFull: Nature Neuroscience Type: main
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