Differential contributions of intra-cellular and inter-cellular mechanisms to the spatial and temporal architecture of the suprachiasmatic nucleus circadian circuitry in wild-type, cryptochrome-null and vasoactive intestinal peptide receptor 2-null mutant mice
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| Title: | Differential contributions of intra-cellular and inter-cellular mechanisms to the spatial and temporal architecture of the suprachiasmatic nucleus circadian circuitry in wild-type, cryptochrome-null and vasoactive intestinal peptide receptor 2-null mutant mice |
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| Authors: | Pauls, S., Foley, N. C., Foley, D. K., LeSauter, J., Hastings, M. H., Maywood, E. S., Silver, R. |
| Source: | European Journal of Neuroscience. Aug2014, Vol. 40 Issue 3, p2528-2540. 13p. 7 Graphs. |
| Subjects: | Circadian rhythms, Suprachiasmatic nucleus, Cryptochromes, Vasoactive intestinal peptide, Electrophysiology, Bioluminescence |
| Abstract: | To serve as a robust internal circadian clock, the cell-autonomous molecular and electrophysiological activities of the individual neurons of the mammalian suprachiasmatic nucleus ( SCN) are coordinated in time and neuroanatomical space. Although the contributions of the chemical and electrical interconnections between neurons are essential to this circuit-level orchestration, the features upon which they operate to confer robustness to the ensemble signal are not known. To address this, we applied several methods to deconstruct the interactions between the spatial and temporal organisation of circadian oscillations in organotypic slices from mice with circadian abnormalities. We studied the SCN of mice lacking Cryptochrome genes ( Cry1 and Cry2), which are essential for cell-autonomous oscillation, and the SCN of mice lacking the vasoactive intestinal peptide receptor 2 (VPAC2-null), which is necessary for circuit-level integration, in order to map biological mechanisms to the revealed oscillatory features. The SCN of wild-type mice showed a strong link between the temporal rhythm of the bioluminescence profiles of PER2:: LUC and regularly repeated spatially organised oscillation. The Cry-null SCN had stable spatial organisation but lacked temporal organisation, whereas in VPAC2-null SCN some specimens exhibited temporal organisation in the absence of spatial organisation. The results indicated that spatial and temporal organisation were separable, that they may have different mechanistic origins (cell-autonomous vs. interneuronal signaling) and that both were necessary to maintain robust and organised circadian rhythms throughout the SCN. This study therefore provided evidence that the coherent emergent properties of the neuronal circuitry, revealed in the spatially organised clusters, were essential to the pacemaking function of the SCN. [ABSTRACT FROM AUTHOR] |
| Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 97319757 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Differential contributions of intra-cellular and inter-cellular mechanisms to the spatial and temporal architecture of the suprachiasmatic nucleus circadian circuitry in wild-type, cryptochrome-null and vasoactive intestinal peptide receptor 2-null mutant mice – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Pauls%2C+S%2E%22">Pauls, S.</searchLink><br /><searchLink fieldCode="AR" term="%22Foley%2C+N%2E+C%2E%22">Foley, N. C.</searchLink><br /><searchLink fieldCode="AR" term="%22Foley%2C+D%2E+K%2E%22">Foley, D. K.</searchLink><br /><searchLink fieldCode="AR" term="%22LeSauter%2C+J%2E%22">LeSauter, J.</searchLink><br /><searchLink fieldCode="AR" term="%22Hastings%2C+M%2E+H%2E%22">Hastings, M. H.</searchLink><br /><searchLink fieldCode="AR" term="%22Maywood%2C+E%2E+S%2E%22">Maywood, E. S.</searchLink><br /><searchLink fieldCode="AR" term="%22Silver%2C+R%2E%22">Silver, R.</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Aug2014, Vol. 40 Issue 3, p2528-2540. 13p. 7 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Circadian+rhythms%22">Circadian rhythms</searchLink><br /><searchLink fieldCode="DE" term="%22Suprachiasmatic+nucleus%22">Suprachiasmatic nucleus</searchLink><br /><searchLink fieldCode="DE" term="%22Cryptochromes%22">Cryptochromes</searchLink><br /><searchLink fieldCode="DE" term="%22Vasoactive+intestinal+peptide%22">Vasoactive intestinal peptide</searchLink><br /><searchLink fieldCode="DE" term="%22Electrophysiology%22">Electrophysiology</searchLink><br /><searchLink fieldCode="DE" term="%22Bioluminescence%22">Bioluminescence</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: To serve as a robust internal circadian clock, the cell-autonomous molecular and electrophysiological activities of the individual neurons of the mammalian suprachiasmatic nucleus ( SCN) are coordinated in time and neuroanatomical space. Although the contributions of the chemical and electrical interconnections between neurons are essential to this circuit-level orchestration, the features upon which they operate to confer robustness to the ensemble signal are not known. To address this, we applied several methods to deconstruct the interactions between the spatial and temporal organisation of circadian oscillations in organotypic slices from mice with circadian abnormalities. We studied the SCN of mice lacking Cryptochrome genes ( Cry1 and Cry2), which are essential for cell-autonomous oscillation, and the SCN of mice lacking the vasoactive intestinal peptide receptor 2 (VPAC2-null), which is necessary for circuit-level integration, in order to map biological mechanisms to the revealed oscillatory features. The SCN of wild-type mice showed a strong link between the temporal rhythm of the bioluminescence profiles of PER2:: LUC and regularly repeated spatially organised oscillation. The Cry-null SCN had stable spatial organisation but lacked temporal organisation, whereas in VPAC2-null SCN some specimens exhibited temporal organisation in the absence of spatial organisation. The results indicated that spatial and temporal organisation were separable, that they may have different mechanistic origins (cell-autonomous vs. interneuronal signaling) and that both were necessary to maintain robust and organised circadian rhythms throughout the SCN. This study therefore provided evidence that the coherent emergent properties of the neuronal circuitry, revealed in the spatially organised clusters, were essential to the pacemaking function of the SCN. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/ejn.12631 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 2528 Subjects: – SubjectFull: Circadian rhythms Type: general – SubjectFull: Suprachiasmatic nucleus Type: general – SubjectFull: Cryptochromes Type: general – SubjectFull: Vasoactive intestinal peptide Type: general – SubjectFull: Electrophysiology Type: general – SubjectFull: Bioluminescence Type: general Titles: – TitleFull: Differential contributions of intra-cellular and inter-cellular mechanisms to the spatial and temporal architecture of the suprachiasmatic nucleus circadian circuitry in wild-type, cryptochrome-null and vasoactive intestinal peptide receptor 2-null mutant mice Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Pauls, S. – PersonEntity: Name: NameFull: Foley, N. C. – PersonEntity: Name: NameFull: Foley, D. K. – PersonEntity: Name: NameFull: LeSauter, J. – PersonEntity: Name: NameFull: Hastings, M. H. – PersonEntity: Name: NameFull: Maywood, E. S. – PersonEntity: Name: NameFull: Silver, R. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 0953816X Numbering: – Type: volume Value: 40 – Type: issue Value: 3 Titles: – TitleFull: European Journal of Neuroscience Type: main |
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