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EVALUATION OF GENOTOXICITY OF ENVIRONMENTALLY FRIENDLY PESTICIDES ON HIGHER CELLS USING THE SINGLE CELL GEL ELECTROPHORESIS (SCGE) ASSAY.

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Bibliographic Details
Title: EVALUATION OF GENOTOXICITY OF ENVIRONMENTALLY FRIENDLY PESTICIDES ON HIGHER CELLS USING THE SINGLE CELL GEL ELECTROPHORESIS (SCGE) ASSAY.
Authors: Dikilitas, Murat1 m.dikilitas@gmail.com, Kocyigit, Abdurrahim2, Bilinc, Hasan3, Taskin, Abdullah3, Karakas, Serna4
Source: Fresenius Environmental Bulletin. 2015, Vol. 24 Issue 1, p39-47. 9p.
Subject Terms: *Genetic toxicology, *Pesticides, *Herbicides, *Fungicides, Gel electrophoresis, Mononuclear leukocytes
Abstract: In this study, the genotoxic effects of commonly used eco-friendly pesticides selected from herbicide (tribenuron methyl), fungicide (triadimenol) and insecticide (lambdacyhalothrin) were evaluated on mononuclear leukocytes isolated from higher cells using the single cell gel electrophoresis (SCGE) assay. Mononuclear cells were exposed for a period of 30 min to 50-, 25-, 15-, 10- and 5 µg ml-1 doses of lambda-cyhalothrin and triadimenol while tribenuron methyl was applied at 10-, 7.5-, 5-, 2.5- and 1 µg ml-1 doses on those cells. DNA damages characterized with Olive tail moment (OTM), comet length and % Tail DNA (% TDNA) increased while % Head DNA (% H-DNA) values significantly decreased with the increase of concentrations of all pesticides used. The results showed that the pesticide lambda-cyhalothrin was found more genotoxic than triadimenol and tribenuron methyl at all doses. However, the lowest doses of triadimenol and tribenuron methyl (last two doses) had only marginal DNA damaging effects on the leukocytes while lambda-cyhalothrin had a remarkable effect at 10 µg ml-1. We conclude that the pesticide lambda-cyhalothrin had a toxic effect on human and could have significant impact on environmental health even if they are applied at low doses. It would be crucially important to determine the effects of genotoxicity and oxidative stress of those pesticides with descending doses on higher cells as well as on plants and animals with extended incubation period if time creates extensive DNA damage. [ABSTRACT FROM AUTHOR]
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Abstract:In this study, the genotoxic effects of commonly used eco-friendly pesticides selected from herbicide (tribenuron methyl), fungicide (triadimenol) and insecticide (lambdacyhalothrin) were evaluated on mononuclear leukocytes isolated from higher cells using the single cell gel electrophoresis (SCGE) assay. Mononuclear cells were exposed for a period of 30 min to 50-, 25-, 15-, 10- and 5 µg ml-1 doses of lambda-cyhalothrin and triadimenol while tribenuron methyl was applied at 10-, 7.5-, 5-, 2.5- and 1 µg ml-1 doses on those cells. DNA damages characterized with Olive tail moment (OTM), comet length and % Tail DNA (% TDNA) increased while % Head DNA (% H-DNA) values significantly decreased with the increase of concentrations of all pesticides used. The results showed that the pesticide lambda-cyhalothrin was found more genotoxic than triadimenol and tribenuron methyl at all doses. However, the lowest doses of triadimenol and tribenuron methyl (last two doses) had only marginal DNA damaging effects on the leukocytes while lambda-cyhalothrin had a remarkable effect at 10 µg ml-1. We conclude that the pesticide lambda-cyhalothrin had a toxic effect on human and could have significant impact on environmental health even if they are applied at low doses. It would be crucially important to determine the effects of genotoxicity and oxidative stress of those pesticides with descending doses on higher cells as well as on plants and animals with extended incubation period if time creates extensive DNA damage. [ABSTRACT FROM AUTHOR]
ISSN:10184619