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Fly-Tox: A panel of transgenic flies expressing pest and pollinator cytochrome P450s.

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Title: Fly-Tox: A panel of transgenic flies expressing pest and pollinator cytochrome P450s.
Authors: McLeman, Amy1 (AUTHOR), Troczka, Bartlomiej J.1 (AUTHOR) b.troczka@exeter.ac.uk, Homem, Rafael A.1,2 (AUTHOR), Duarte, Ana1 (AUTHOR), Zimmer, Christoph1 (AUTHOR), Garrood, William T.1,2 (AUTHOR), Pym, Adam1 (AUTHOR), Beadle, Katherine1 (AUTHOR), Reid, Rebecca J.2 (AUTHOR), Douris, Vassilis3,4 (AUTHOR), Vontas, John3,5 (AUTHOR), Davies, T.G. Emyr2 (AUTHOR), ffrench Constant, Richard1 (AUTHOR), Nauen, Ralf6 (AUTHOR), Bass, Chris1 (AUTHOR) c.bass@exeter.ac.uk
Source: Pesticide Biochemistry & Physiology. Oct2020, Vol. 169, pN.PAG-N.PAG. 1p.
Subject Terms: *Pollinators, *Pests, *Insecticides, *Insect pests, Insect pollinators, Drosophila melanogaster, Flies, Bees
Abstract: There is an on-going need to develop new insecticides that are not compromised by resistance and that have improved environmental profiles. However, the cost of developing novel compounds has increased significantly over the last two decades. This is in part due to increased regulatory requirements, including the need to screen both pest and pollinator insect species to ensure that pre-existing resistance will not hamper the efficacy of a new insecticide via cross-resistance, or adversely affect non-target insect species. To add to this problem the collection and maintenance of toxicologically relevant pest and pollinator species and strains is costly and often difficult. Here we present Fly-Tox, a panel of publicly available transgenic Drosophila melanogaster lines each containing one or more pest or pollinator P450 genes that have been previously shown to metabolise insecticides. We describe the range of ways these tools can be used, including in predictive screens to avoid pre-existing cross-resistance, to identify potential resistance-breaking inhibitors, in the initial assessment of potential insecticide toxicity to bee pollinators, and identifying harmful pesticide-pesticide interactions. Unlabelled Image • Drosophila melanogaster is a powerful model system to study cytochrome P450-mediated insecticide tolerance. • Fly-tox comprises a panel of transgenic D. melanogaster lines expressing P450s from insect pest and pollinator species. • Fly-tox can be used in numerous ways to characterise the role of pest/pollinator P450s in insecticide metabolism. • We describe the applications of Fly-Tox and provide detailed protocols on its use. • The benefits and limitations of the system are discussed. [ABSTRACT FROM AUTHOR]
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Abstract:There is an on-going need to develop new insecticides that are not compromised by resistance and that have improved environmental profiles. However, the cost of developing novel compounds has increased significantly over the last two decades. This is in part due to increased regulatory requirements, including the need to screen both pest and pollinator insect species to ensure that pre-existing resistance will not hamper the efficacy of a new insecticide via cross-resistance, or adversely affect non-target insect species. To add to this problem the collection and maintenance of toxicologically relevant pest and pollinator species and strains is costly and often difficult. Here we present Fly-Tox, a panel of publicly available transgenic Drosophila melanogaster lines each containing one or more pest or pollinator P450 genes that have been previously shown to metabolise insecticides. We describe the range of ways these tools can be used, including in predictive screens to avoid pre-existing cross-resistance, to identify potential resistance-breaking inhibitors, in the initial assessment of potential insecticide toxicity to bee pollinators, and identifying harmful pesticide-pesticide interactions. Unlabelled Image • Drosophila melanogaster is a powerful model system to study cytochrome P450-mediated insecticide tolerance. • Fly-tox comprises a panel of transgenic D. melanogaster lines expressing P450s from insect pest and pollinator species. • Fly-tox can be used in numerous ways to characterise the role of pest/pollinator P450s in insecticide metabolism. • We describe the applications of Fly-Tox and provide detailed protocols on its use. • The benefits and limitations of the system are discussed. [ABSTRACT FROM AUTHOR]
ISSN:00483575
DOI:10.1016/j.pestbp.2020.104674