View in EDS

Fly-Tox: A panel of transgenic flies expressing pest and pollinator cytochrome P450s.

Saved in:
Bibliographic Details
Title: Fly-Tox: A panel of transgenic flies expressing pest and pollinator cytochrome P450s.
Authors: McLeman, Amy1 (AUTHOR), Troczka, Bartlomiej J.1 (AUTHOR) b.troczka@exeter.ac.uk, Homem, Rafael A.1,2 (AUTHOR), Duarte, Ana1 (AUTHOR), Zimmer, Christoph1 (AUTHOR), Garrood, William T.1,2 (AUTHOR), Pym, Adam1 (AUTHOR), Beadle, Katherine1 (AUTHOR), Reid, Rebecca J.2 (AUTHOR), Douris, Vassilis3,4 (AUTHOR), Vontas, John3,5 (AUTHOR), Davies, T.G. Emyr2 (AUTHOR), ffrench Constant, Richard1 (AUTHOR), Nauen, Ralf6 (AUTHOR), Bass, Chris1 (AUTHOR) c.bass@exeter.ac.uk
Source: Pesticide Biochemistry & Physiology. Oct2020, Vol. 169, pN.PAG-N.PAG. 1p.
Subject Terms: *Pollinators, *Pests, *Insecticides, *Insect pests, Insect pollinators, Drosophila melanogaster, Flies, Bees
Abstract: There is an on-going need to develop new insecticides that are not compromised by resistance and that have improved environmental profiles. However, the cost of developing novel compounds has increased significantly over the last two decades. This is in part due to increased regulatory requirements, including the need to screen both pest and pollinator insect species to ensure that pre-existing resistance will not hamper the efficacy of a new insecticide via cross-resistance, or adversely affect non-target insect species. To add to this problem the collection and maintenance of toxicologically relevant pest and pollinator species and strains is costly and often difficult. Here we present Fly-Tox, a panel of publicly available transgenic Drosophila melanogaster lines each containing one or more pest or pollinator P450 genes that have been previously shown to metabolise insecticides. We describe the range of ways these tools can be used, including in predictive screens to avoid pre-existing cross-resistance, to identify potential resistance-breaking inhibitors, in the initial assessment of potential insecticide toxicity to bee pollinators, and identifying harmful pesticide-pesticide interactions. Unlabelled Image • Drosophila melanogaster is a powerful model system to study cytochrome P450-mediated insecticide tolerance. • Fly-tox comprises a panel of transgenic D. melanogaster lines expressing P450s from insect pest and pollinator species. • Fly-tox can be used in numerous ways to characterise the role of pest/pollinator P450s in insecticide metabolism. • We describe the applications of Fly-Tox and provide detailed protocols on its use. • The benefits and limitations of the system are discussed. [ABSTRACT FROM AUTHOR]
Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: GreenFILE
FullText Text:
  Availability: 0
Header DbId: 8gh
DbLabel: GreenFILE
An: 145443913
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Fly-Tox: A panel of transgenic flies expressing pest and pollinator cytochrome P450s.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22McLeman%2C+Amy%22">McLeman, Amy</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Troczka%2C+Bartlomiej+J%2E%22">Troczka, Bartlomiej J.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> b.troczka@exeter.ac.uk</i><br /><searchLink fieldCode="AR" term="%22Homem%2C+Rafael+A%2E%22">Homem, Rafael A.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Duarte%2C+Ana%22">Duarte, Ana</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zimmer%2C+Christoph%22">Zimmer, Christoph</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Garrood%2C+William+T%2E%22">Garrood, William T.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pym%2C+Adam%22">Pym, Adam</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Beadle%2C+Katherine%22">Beadle, Katherine</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Reid%2C+Rebecca+J%2E%22">Reid, Rebecca J.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Douris%2C+Vassilis%22">Douris, Vassilis</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vontas%2C+John%22">Vontas, John</searchLink><relatesTo>3,5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Davies%2C+T%2EG%2E+Emyr%22">Davies, T.G. Emyr</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22ffrench+Constant%2C+Richard%22">ffrench Constant, Richard</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nauen%2C+Ralf%22">Nauen, Ralf</searchLink><relatesTo>6</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bass%2C+Chris%22">Bass, Chris</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> c.bass@exeter.ac.uk</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Pesticide+Biochemistry+%26+Physiology%22">Pesticide Biochemistry & Physiology</searchLink>. Oct2020, Vol. 169, pN.PAG-N.PAG. 1p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: *<searchLink fieldCode="DE" term="%22Pollinators%22">Pollinators</searchLink><br />*<searchLink fieldCode="DE" term="%22Pests%22">Pests</searchLink><br />*<searchLink fieldCode="DE" term="%22Insecticides%22">Insecticides</searchLink><br />*<searchLink fieldCode="DE" term="%22Insect+pests%22">Insect pests</searchLink><br /><searchLink fieldCode="DE" term="%22Insect+pollinators%22">Insect pollinators</searchLink><br /><searchLink fieldCode="DE" term="%22Drosophila+melanogaster%22">Drosophila melanogaster</searchLink><br /><searchLink fieldCode="DE" term="%22Flies%22">Flies</searchLink><br /><searchLink fieldCode="DE" term="%22Bees%22">Bees</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: There is an on-going need to develop new insecticides that are not compromised by resistance and that have improved environmental profiles. However, the cost of developing novel compounds has increased significantly over the last two decades. This is in part due to increased regulatory requirements, including the need to screen both pest and pollinator insect species to ensure that pre-existing resistance will not hamper the efficacy of a new insecticide via cross-resistance, or adversely affect non-target insect species. To add to this problem the collection and maintenance of toxicologically relevant pest and pollinator species and strains is costly and often difficult. Here we present Fly-Tox, a panel of publicly available transgenic Drosophila melanogaster lines each containing one or more pest or pollinator P450 genes that have been previously shown to metabolise insecticides. We describe the range of ways these tools can be used, including in predictive screens to avoid pre-existing cross-resistance, to identify potential resistance-breaking inhibitors, in the initial assessment of potential insecticide toxicity to bee pollinators, and identifying harmful pesticide-pesticide interactions. Unlabelled Image • Drosophila melanogaster is a powerful model system to study cytochrome P450-mediated insecticide tolerance. • Fly-tox comprises a panel of transgenic D. melanogaster lines expressing P450s from insect pest and pollinator species. • Fly-tox can be used in numerous ways to characterise the role of pest/pollinator P450s in insecticide metabolism. • We describe the applications of Fly-Tox and provide detailed protocols on its use. • The benefits and limitations of the system are discussed. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=8gh&AN=145443913
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.pestbp.2020.104674
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Pollinators
        Type: general
      – SubjectFull: Pests
        Type: general
      – SubjectFull: Insecticides
        Type: general
      – SubjectFull: Insect pests
        Type: general
      – SubjectFull: Insect pollinators
        Type: general
      – SubjectFull: Drosophila melanogaster
        Type: general
      – SubjectFull: Flies
        Type: general
      – SubjectFull: Bees
        Type: general
    Titles:
      – TitleFull: Fly-Tox: A panel of transgenic flies expressing pest and pollinator cytochrome P450s.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: McLeman, Amy
      – PersonEntity:
          Name:
            NameFull: Troczka, Bartlomiej J.
      – PersonEntity:
          Name:
            NameFull: Homem, Rafael A.
      – PersonEntity:
          Name:
            NameFull: Duarte, Ana
      – PersonEntity:
          Name:
            NameFull: Zimmer, Christoph
      – PersonEntity:
          Name:
            NameFull: Garrood, William T.
      – PersonEntity:
          Name:
            NameFull: Pym, Adam
      – PersonEntity:
          Name:
            NameFull: Beadle, Katherine
      – PersonEntity:
          Name:
            NameFull: Reid, Rebecca J.
      – PersonEntity:
          Name:
            NameFull: Douris, Vassilis
      – PersonEntity:
          Name:
            NameFull: Vontas, John
      – PersonEntity:
          Name:
            NameFull: Davies, T.G. Emyr
      – PersonEntity:
          Name:
            NameFull: ffrench Constant, Richard
      – PersonEntity:
          Name:
            NameFull: Nauen, Ralf
      – PersonEntity:
          Name:
            NameFull: Bass, Chris
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 10
              Text: Oct2020
              Type: published
              Y: 2020
          Identifiers:
            – Type: issn-print
              Value: 00483575
          Numbering:
            – Type: volume
              Value: 169
          Titles:
            – TitleFull: Pesticide Biochemistry & Physiology
              Type: main
ResultId 1