DNA templates with blocked long 3' end single-stranded overhangs (BL3SSO) promote bona fide Cas9-stimulated homology-directed repair of long transgenes into endogenous gene loci.

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Title: DNA templates with blocked long 3' end single-stranded overhangs (BL3SSO) promote bona fide Cas9-stimulated homology-directed repair of long transgenes into endogenous gene loci.
Authors: Bandyopadhyay, Saptaparni1, Douglass, Joseph1, Kapell, Sebastian1,2, Khan, Nazimuddin1,2, Feitosa-Suntheimer, Fabiana2, Klein, Jenny A.3,4, Temple, Jasmine3, Brown-Culbertson, Jayce1, Tavares, Alexander H.1,2, Saeed, Mohsan1,2 msaeed1@bu.edu, Lau, Nelson C.1,2,5 nclau@bu.edu
Source: G3: Genes | Genomes | Genetics. Aug2021, Vol. 11 Issue 8, p1-16. 16p.
Database: Academic Search Ultimate
Description
ISSN:21601836
DOI:10.1093/g3journal/jkab169