View in EDS

Microchip system for monitoring microbial physiological behaviour under drug influences.

Saved in:
Bibliographic Details
Title: Microchip system for monitoring microbial physiological behaviour under drug influences.
Authors: Arora S (AUTHOR), Lim CS (AUTHOR), Foo JY (AUTHOR), Sakharkar MK (AUTHOR), Dixit P (AUTHOR), Liu AQ (AUTHOR), Miao JM (AUTHOR), Arora, S1 (AUTHOR), Lim, C S (AUTHOR), Foo, J Y (AUTHOR), Sakharkar, M K (AUTHOR), Dixit, P (AUTHOR), Liu, A Q (AUTHOR), Miao, J M (AUTHOR)
Source: Proceedings of the Institution of Mechanical Engineers -- Part H -- Journal of Engineering in Medicine (Professional Engineering Publishing). 2009 Aug, Vol. 223 Issue 6, p777-786. 10p.
Abstract: Single-step real-time high-throughput monitoring of drug influences on bacterial cell behaviour has become important with growing interests in personalized therapy and medication. Conventional microchip assemblies to perform similar work do exist. However, most of these devices have complex set-ups incorporating micromixers, separators, pumps, or valves. These microcomponents can sometimes damage the entities being monitored because of the creation of unfavourable biological environments. This paper presents a microchip-based system that enables single-step mixing of two solutions in various ratios, without the need for additional microcomponents such as mixers and pumps, in order to screen effectively their combinatory effects on cell outcomes. In this work, in-vitro experiments were carried out using ampicillin at various concentrations to investigate their effects on Escherichia coli (E. coli). Results showed that the microchip provided effective screening, which yielded useful results such as effective dosages, ineffective dosages, and other possible outcomes; for instance, in this case, the occurrence of adaptive mutation of the bacteria at certain drug concentrations. Comparative microbiological laboratory tests were carried out as standard for confirmation of the results. [ABSTRACT FROM AUTHOR]
Copyright of Proceedings of the Institution of Mechanical Engineers -- Part H -- Journal of Engineering in Medicine (Professional Engineering Publishing) is the property of Professional Engineering Publishing and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:Single-step real-time high-throughput monitoring of drug influences on bacterial cell behaviour has become important with growing interests in personalized therapy and medication. Conventional microchip assemblies to perform similar work do exist. However, most of these devices have complex set-ups incorporating micromixers, separators, pumps, or valves. These microcomponents can sometimes damage the entities being monitored because of the creation of unfavourable biological environments. This paper presents a microchip-based system that enables single-step mixing of two solutions in various ratios, without the need for additional microcomponents such as mixers and pumps, in order to screen effectively their combinatory effects on cell outcomes. In this work, in-vitro experiments were carried out using ampicillin at various concentrations to investigate their effects on Escherichia coli (E. coli). Results showed that the microchip provided effective screening, which yielded useful results such as effective dosages, ineffective dosages, and other possible outcomes; for instance, in this case, the occurrence of adaptive mutation of the bacteria at certain drug concentrations. Comparative microbiological laboratory tests were carried out as standard for confirmation of the results. [ABSTRACT FROM AUTHOR]
ISSN:09544119