Production of PHB from Chicory Roots - Comparison of Three Cupriavidus necator Strains.

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Bibliographic Details
Title:	Production of PHB from Chicory Roots - Comparison of Three Cupriavidus necator Strains.
Authors:	Follonier, S.¹, Riesen, R.², Zinn, M.¹ manfred.zinn@hevs.ch
Source:	Chemical & Biochemical Engineering Quarterly. Jul2015, Vol. 29 Issue 2, p99-112. 14p.
Subjects:	Polyesters, Microbial polymers, Grapes, Fatty acids, Biosynthesis, Pseudomonas putida
Abstract:	Chicory roots from hydroponic salad cultivation are an abundant food residue in Navarra (Spain) that are underutilized to date. Aiming at a holistic utilization of resources, we report here the first process using chicory root hydrolysate for the production of poly([R]-3-hydroxybutyrate) (PHB). The polymer can be used for packaging material made for the locally produced vegetables. In the first step, we developed a pre-treatment process to obtain a hydrolysate, which contained 34 g L-1 sugars and 0.7 g L-1 total Kjeldahl nitrogen. This hydrolysate was used as fermentation substrate for three PHB-producing strains. Cupriavidus necator DSM 428 reached a dry biomass concentration of 11.3 g L-1 with a PHB content of 66% in dry mass within 5 days. C. necator DSM 531 yielded 3.5 g L-1 dry biomass containing 46% PHB within the same period. C. necator DSM 545 was superior over the other two in that 14.0 g L-1 of biomass containing 78% PHB after only 3 days were obtained. These results show that even within the same species, the productivities on natural substrates are very different. The produced polymers were extracted using chloroform, and several thermo-physical parameters are in good accordance with published data. Overall, our holistic approach and the encouraging results prove that chicory roots are a viable fermentation substrate for PHB-production. [ABSTRACT FROM AUTHOR]
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Database:	Engineering Source

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Abstract:	Chicory roots from hydroponic salad cultivation are an abundant food residue in Navarra (Spain) that are underutilized to date. Aiming at a holistic utilization of resources, we report here the first process using chicory root hydrolysate for the production of poly([R]-3-hydroxybutyrate) (PHB). The polymer can be used for packaging material made for the locally produced vegetables. In the first step, we developed a pre-treatment process to obtain a hydrolysate, which contained 34 g L-1 sugars and 0.7 g L-1 total Kjeldahl nitrogen. This hydrolysate was used as fermentation substrate for three PHB-producing strains. Cupriavidus necator DSM 428 reached a dry biomass concentration of 11.3 g L-1 with a PHB content of 66% in dry mass within 5 days. C. necator DSM 531 yielded 3.5 g L-1 dry biomass containing 46% PHB within the same period. C. necator DSM 545 was superior over the other two in that 14.0 g L-1 of biomass containing 78% PHB after only 3 days were obtained. These results show that even within the same species, the productivities on natural substrates are very different. The produced polymers were extracted using chloroform, and several thermo-physical parameters are in good accordance with published data. Overall, our holistic approach and the encouraging results prove that chicory roots are a viable fermentation substrate for PHB-production. [ABSTRACT FROM AUTHOR]
ISSN:	03529568
DOI:	10.15255/CABEQ.2014.2250