Bibliographic Details
| Title: |
Crystallization and initial X-ray diffraction studies of higher plant photosystem I. |
| Authors: |
Ben-Shem, Adam1 adam@post.tau.ac.il, Nelson, Nathan1, Friolow, Felix2 |
| Source: |
Acta Crystallographica: Section D (Wiley-Blackwell). Oct2003, Vol. 59 Issue 10, p1824. 4p. |
| Subjects: |
Crystallization, Peas, Synchrotron radiation, Isomorphism (Crystallography), X-ray diffraction, Patterson function |
| Abstract: |
Complete photosystem I from a higher plant (pea; Pisum sativum) was isolated, purified and crystallized. The crystals diffract to 4 Å resolution using synchrotron radiation and belong to the monoclinic crystal system, space group P2[SUB1], with unit-cell parameters a = 181.90, b = 190.24, c = 219.66 Å, Β = 90.484°. Data sets from three crystals have been collected and were merged into a 4.4 Å resolution data set, taing advantage of the high isomorphicity observed for these crystals. Analysis of the self-rotation Patterson function suggests the possible presence of two PSI complexes in the asymmetric unit. [ABSTRACT FROM AUTHOR] |
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| Database: |
Engineering Source |