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Regulation of MuSK Expression by a Novel Signaling Pathway.

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Title: Regulation of MuSK Expression by a Novel Signaling Pathway.
Authors: Chang-Hoon Kim1, Xiong, Wen C.2 wxiong@path.uab.edu, Lin Me, Wen C.1 lmei@nrc.uab.edu
Source: Journal of Biological Chemistry. 10/3/2003, Vol. 278 Issue 40, p38522-38527. 6p. 5 Diagrams, 8 Graphs.
Subjects: Protein-tyrosine kinases, Gene expression, Genetic transcription, Laboratory mice
Abstract: MuSK is a receptor tyrosine kinase essential for neuromuscular junction formation. Expression of the MuSK gene is tightly regulated during development and at the neuromuscular junction. However, little is known about molecular mechanisms regulating its gene expression. Here we report a characterization of the promoter of the mouse MuSK gene. The transcription of MuSK starts at multiple sites with a major site 51 nt upstream of the translation start site. We have identified an E-box-like cis-element that is both required and sufficient for differentiation-dependent transcription. Interestingly, the promoter activity of the MuSK gene did not respond to neuregulin, a factor believed to mediate the synapse-specific transcription of acetylcholine receptor subunit genes. Rather, MuSK expression is increased in muscle cells stimulated with Wnt or at conditions when the Wnt signaling was activated. These results suggest a novel mechanism for the MuSK synapse-specific expression. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:MuSK is a receptor tyrosine kinase essential for neuromuscular junction formation. Expression of the MuSK gene is tightly regulated during development and at the neuromuscular junction. However, little is known about molecular mechanisms regulating its gene expression. Here we report a characterization of the promoter of the mouse MuSK gene. The transcription of MuSK starts at multiple sites with a major site 51 nt upstream of the translation start site. We have identified an E-box-like cis-element that is both required and sufficient for differentiation-dependent transcription. Interestingly, the promoter activity of the MuSK gene did not respond to neuregulin, a factor believed to mediate the synapse-specific transcription of acetylcholine receptor subunit genes. Rather, MuSK expression is increased in muscle cells stimulated with Wnt or at conditions when the Wnt signaling was activated. These results suggest a novel mechanism for the MuSK synapse-specific expression. [ABSTRACT FROM AUTHOR]
ISSN:00219258
DOI:10.1074/jbc.M305058200